Cloning and Functional Analysis of a miRNA（Bra-miR07）Associated with the Regulation of Early Organ Development in Cauliflower

doi:10.16420/j.issn.0513-353x.2015-0855

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (1): 141-150.doi: 10.16420/j.issn.0513-353x.2015-0855

• Research Notes • Previous Articles Next Articles

Cloning and Functional Analysis of a miRNA（Bra-miR07）Associated with the Regulation of Early Organ Development in Cauliflower

ZHANG Qing-li1，JIN Chuan1，QIN Er-jun1，WANG Yu1，WU Mei1，LI Li-hong1，LI Hui2，CHEN Cheng-bin1，SONG Wen-qin1，and WANG Chun-guo1,*

1College of Life Sciences，Nankai University，Tianjin 300071，China；2College of Horticulture and Landscape，Tianjin Agricultural University，Tianjin 300384，China

Online:2016-01-25 Published:2016-01-25

Abstract

Abstract:

In the previous study，a series of novel miRNAs that show significantly different expression in the early organ development of cauliflower were obtained by high-throughput sequencing. In the present study，a novel miRNA that showed different expression between cauliflower hypocotyl and cotyledon was named Bra-miR07 and further analyzed. The results indicated that the sequence length of Bra-miR07 precursor was 270 bp which can form the stable secondary hairpin structure. Bra-miR07 appeared higher expression in cauliflower cotyledon than that in hypocotyl. To further uncover the function of Bra-miR07，overexpression vector of Bra-miR07 was constructed and transferred into Arabidopsis thaliana by floral dip，and the Bra-miR07 overexpression transgenic plants were successfully obtained. Phenotypic analyses showed that overexpression of Bra-miR07 can significantly influence thegrowth and development of transgenic plants. Compared with wild-type Arabidopsis，Bra-miR07 transgenic plants showed slow growth，dwarf plant and lower fertility. Consequently，these results implied that Bra-miR07 may also play important roles in regulation of cauliflower organ development.

Key words: cauliflower, miRNA, Bra-miR07, miRNA function, organ development

CLC Number:

S 635.3

ZHANG Qing-li1，JIN Chuan1，QIN Er-jun1，WANG Yu1，WU Mei1，LI Li-hong1，LI Hui2，CHEN Cheng-bin1，SONG Wen-qin1，and WANG Chun-guo1,*. Cloning and Functional Analysis of a miRNA（Bra-miR07）Associated with the Regulation of Early Organ Development in Cauliflower[J]. ACTA HORTICULTURAE SINICA, 2016, 43(1): 141-150.

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Cloning and Functional Analysis of a miRNA（Bra-miR07）Associated with the Regulation of Early Organ Development in Cauliflower

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