SRAP Markers for Resistance to Nematode（Meloidogyne incognita）of Prunus kansuensis

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (7): 1057-1064.

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SRAP Markers for Resistance to Nematode（Meloidogyne incognita）of Prunus kansuensis

LIU Wei1.2，CAO Ke2，WANG Li-rong2,*，ZHANG Shao-ling1，ZHU Geng-rui2，FANG Wei-chao2，and CHEN Chang-wen2

（1College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China；2Zhengzhou Fruit Research Institute，Chinese Acdemy of Agricultutal Sciences，Zhengzhou 450009，China）

Received:2009-09-17 Revised:2010-06-22 Online:2010-07-25 Published:2010-07-25
Contact: WANG Li-rong

Abstract

Abstract: A linkage map was obtained using a 190 BC1 progeny{Prunus persica（L.）Batsch. × [P. persica（L.）Batsch. × P. kansuensis]}. The selected primers 53 SRAP with rich polymorphism and steady bands were tested in progeny. One hundred and fifteen markers in 1︰1 ratio χ2 test were integrated in the map with Mapmaker/Exp 3.0 software. The map is composed of 103 loci（102 SRAP，1 root-knot nematodes resistance traits） distributed in 8 linkage groups. The map covers 994.2 cM of the peach genome. The average length of 8 groups is 124.3 cM，the average distance between adjacent loci is 9.6 cM. SRAP markers linked to root-knot nematodes resistance gene，which genetic distance is 2.1 cM，were located in group 5. The 36 F2 progeny was verified the validity. The accuracy rate is 91.7%.

Key words: peach, root-knot nematode, genetic linkage map, SRAP marker

CLC Number:

S 662.1

LIU Wei.;CAO Ke;WANG Li-rong;ZHANG Shao-ling;ZHU Geng-rui;FANG Wei-chao;and CHEN Chang-wen. SRAP Markers for Resistance to Nematode（Meloidogyne incognita）of Prunus kansuensis[J]. ACTA HORTICULTURAE SINICA, 2010, 37(7): 1057-1064.

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SRAP Markers for Resistance to Nematode（Meloidogyne incognita）of Prunus kansuensis

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