Abstract: The use of vinegar residue substrate as component of mixed substrate for pepper seedling growth instead of peat was studied. Vinegar residue substrate，peat and vermiculite were mixed in various ratios. Prior to sowing，some physical and chemical properties of the seedling substrates were determined and the growth of pepper seedling was also studied. In most of the cases，the addition of vinegar residue substrate to the seedling substrate produced an increase in total porosity and aeration porosity，a decrease in water holding porosity，whereas the content of vermiculite in mixed substrates increasing，the water holding porosity was also increased；The nutrient concentrations and electrical conductivity（EC）of vinegar residue substrate were higher than peat and vermiculite，less than 75% vinegar residue substrate using in mixed substrate，the plant height, total leaf area and biomass of pepper seedlings were better than control；The addition of vermiculite to the seedling substrate，which had good water holding capacity，the growth of pepper seedling was improved.

Key words: pepper, vinegar residue substrate, seedling, peat, vermiculite