Abstract: FT gene was cloned by RT-PCR from wild type Arabidopsis. After sequencing, the FT gene with proper digestion sites was digested and inserted into plant expression vector Super1300⁺, and was introduced into Chrysanthemum genome by Agrobacterium-mediated transformation. The integration of
FT was determined using PCR and PCR-Southern, and gene expression was tested by RT-PCR. After transformation, we obtained 29 resistant plants. PCR and PCR-Southern blot analysis showed that eight of them were positive. RT-PCR detected the expression of the exogenous FT gene in the leaves of transgenic plants. One line of eight transplants appeared flowering bud in vitro. It indicated that transplants flowered early without influence of light.

Key words: Cut chrysanthemum, FT gene, transgenic, flowering