Development and Validation of SCAR Markers Linked to the Non-acid /acidTrait of Fruit in Peach

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (8): 1120-1126.

• 果树 • Previous Articles Next Articles

Development and Validation of SCAR Markers Linked to the Non-acid /acidTrait of Fruit in Peach

WU Jun^1,3;SHU Huai-rui¹;ZHANG Kai-chun^2*;Zhang Xiao-ming²;JIANG Li-jie²

(¹College of Horticulture Science and Engineering, Shandong Agricultural University, Tai^，an, Shandong 271018, China;²Institute of Forestry and Pom ology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China;³College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)

Received:2009-02-04 Revised:2009-07-02 Online:2009-08-25 Published:2009-08-25
Contact: ZHANG Kai-chun

Abstract

Abstract: In this paper, specific primers of BFPA1/A2 , BFPB1/B2 , BFPC1/C2 were designed respectively according to the sequence of three specific fragments from AFLP markerswhich linked to non-acid/acid trait of peach. A 158 bp polymorphic band was amplified from the DNA of 69 F¹ population of the intra-specific cross between cultivars‘Jingyu’and‘Flavortop’with the primer pair BFPB1/B2. Furthermore, the steady and specific amp lification can be got with the primers designed in different location of the AFLP fragmentAT-CTA199. The SCAR marker had the same segregation pattern as the original AFLP_AT/CTA marker with a linkage distance of 2199 cM and the AFLP marker was converted into SCAR marker successfully. The SCAR_BFPB1/B2 marker was tested in 60 F²p rogeny of‘Okubo’and‘Xingjin’nectarine, and the results showed that the ac2 cordance ratio of genotype and phenotype was 96.7%. The SCAR marker was a codominant marker and could distinguish the heterozygous and homozygous. Polymorphism disappeared in parents and progeny with specific primers BFPA1 /A2 and BFPC1 /C2.

Key words: peach, non-acid/acid, AFLP, SCAR

CLC Number:

S662.1
Q74

WU Jun;SHU Huai-rui;ZHANG Kai-chun;Zhang Xiao-ming;JIANG Li-jie. Development and Validation of SCAR Markers Linked to the Non-acid /acidTrait of Fruit in Peach[J]. ACTA HORTICULTURAE SINICA, 2009, 36(8): 1120-1126.

[1]	ZHAI Hanhan, ZHAI Yujie, TIAN Yi, ZHANG Ye, YANG Li, WEN Zhiliang, CHEN Haijiang. Genome-wide Identification of Peach SAUR Gene Family and Characterization of PpSAUR5 Gene [J]. Acta Horticulturae Sinica, 2023, 50(1): 1-14.
[2]	XING Zhudong, LÜ Futang, GUO Shangjing, ZHANG Yanyi. A New Peach Cultivar‘Liaoda Hongjin’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 225-226.
[3]	YANG Xingwang, WANG Haibo, WANG Yingying, WANG Xiaolong, WANG Zhiqiang, LIU Peipei, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening and Cold Resistant Peach Cultivar‘Zhongnong Ganshuang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 15-16.
[4]	YANG Xingwang, WANG Haibo, WANG Yingying, ZHANG Yican, WANG Baoliang, Liu Peipei, SHI Xiangbin, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening Cold Resistant Peach Cultivar‘Zhongnong Baigan’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 17-18.
[5]	YANG Xingwang, LIU Fengzhi, WANG Haibo, WANG Yingying, WANG Zhiqiang, SHI Xiangbin, JI Xiaohao, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening Cold Resistant Peach Cultivar‘Zhongnong Hanshuimi’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 19-20.
[6]	YANG Xingwang, LIU Fengzhi, WANG Haibo, WANG Yingying, ZHANG Yican, LI Peng, WANG Xiaolong, LIU Wanchun, and WANG Xiaodi. A New Late-ripening Cold Resistant Peach Cultivar‘Zhongnong Qiuxiang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 21-22.
[7]	WANG Yingying, LIU Lichang, LIU Zhiwu, YANG Xingwang, LIU Wanchun, and WANG Xiaodi, . A New Extremely Late-ripening Peach Cultivar‘Zhongnong Dongmi’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 23-24.
[8]	WANG Yingying, ZHENG Xiaocui, WANG Haibo, SHI Xiangbin, JI Xiaohao, LIU Peipei, LIU Wanchun, and WANG Xiaodi. A New Cold Resistent Peach Cultivar‘Zhongtao Fenyu’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 13-14.
[9]	WANG Yingying, WANG Haibo, LIU Peipei, WANG Baoliang, ZHANG Yican, LI Peng, LIU Wanchun, and WANG Xiaodi. A New Cold Resistant Peach Cultivar‘Zhongnong Xiutian’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 15-16.
[10]	WANG Yingying, WANG Haibo, LI Peng, WANG Baoliang, SHI Xiangbin, LIU Wanchun, and WANG Xiaodi. A New Cold Resistant Peach Cultivar‘Zhongnong Zhihou’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 17-18.
[11]	WANG Yingying, WANG Haibo, SHI Xiangbin, and WANG Xiaodi. A New Early-ripening Peach Cultivar‘Zhongnong Hanmi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 19-20.
[12]	WANG Yuehui, BAI Ruixia, JIA Yunyun, MA Zhisheng, and LI Jianming. A New Mid-ripening Peach Cultivar‘Meilin’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 21-22.
[13]	SHU Nan, FAN Shutian, WANG Yue, XU Peilei, LI Jiaqi, LIU Tao, WANG Xinhua, JIN Yuning, and LU Wenpeng. A New Cold Resistant Peach Cultivar‘Jimei’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 23-24.
[14]	JIANG Yajun, CHEN Jiajia, TAN Bin, ZHENG Xianbo, WANG Wei, ZHANG Langlang, CHENG Jun, FENG Jiancan. Function Exploration of PpIDD11 in Regulating Peach Flower Development [J]. Acta Horticulturae Sinica, 2022, 49(9): 1841-1852.
[15]	ZHANG Meng, SHAN Yuying, YANG Yebo, ZHAI Feifei, WANG Zhaoshan, JU Guansheng, SUN Zhenyuan, LI Zhenjian. AFLP Analysis of Genetic Resources of Dendrobium from China [J]. Acta Horticulturae Sinica, 2022, 49(6): 1339-1350.

Development and Validation of SCAR Markers Linked to the Non-acid /acidTrait of Fruit in Peach

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments