Cloning of Lily symptomless virus CP Gene and Construction of Its RNAi Vector

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (6): 885-890.

• 研究简报 • Previous Articles Next Articles

Cloning of Lily symptomless virus CP Gene and Construction of Its RNAi Vector

XU Pin-san^1*,YIN Ya-lei¹, and LI Yu-hua²

(¹School of Environm ental and B iological Science and Technology, Dalian University of Technology, Dalian, L iaoning 116024,China; ²College of Life Sciences, Northeast Forestry University, Harbin 150040, China)

Received:2009-01-19 Revised:2009-05-11 Online:2009-06-25 Published:2009-06-25
Contact: XU Pin-san

Abstract

Abstract: A fragment of 876 bp of Lily symptomless virus (LSV) CP gene cDNA sequence was amp lified by reverse transcrip tion polymerase chain reaction (RT-PCR) from the total RNA infected lily leaves. The Blast result showed that the sequence p resented a very high match with the LSV CP genes in the GenBank and its homology was 98%. The amino acid sequence was 98% homologus. Using Gateway technology, a entry clone vector (pDONR201) was constructed through the way of BP cloning and a RNA interference (RNAi) transformation vector (pH7GW IWG2) was constructed through the way of LR cloning. The RNAi vector was obtained and could be transformed into lily by Agrobacterium tum efaciens.

Key words: lily, Lily symptomless virus, CP gene, cloning vector, RNA interference, Gateway technology

CLC Number:

XU Pin-san;YIN Ya-lei;and LI Yu-hua. Cloning of Lily symptomless virus CP Gene and Construction of Its RNAi Vector[J]. ACTA HORTICULTURAE SINICA, 2009, 36(6): 885-890.

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Cloning of Lily symptomless virus CP Gene and Construction of Its RNAi Vector

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