Purification and Identification of a Pathogenesis-related Protein in Intercellular Fluids of Cucumber Leaves Induced by BTH

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (4): 507-512.

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Purification and Identification of a Pathogenesis-related Protein in Intercellular Fluids of Cucumber Leaves Induced by BTH

LI Yu-hong¹,CHEN Peng^2*,CHENG Zhi-hui¹, ZHANG Jing¹, and DONG Jian³

(¹College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China; ²College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China; ³College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China)

Received:2008-10-17 Revised:2009-03-17 Online:2009-04-25 Published:2009-04-25
Contact: CHEN Peng

Abstract

Abstract:

This article reported the induction, purification and identification of a pathogenesis-related protein from the intercellular fluids of cucumber seedlings, which were treated with benzothiadiazole (BTH) or
Pseudoperonospora cubensis. Our results demonstrated that both BTH treatment and Pseudoperonospora cubensis inoculation could induce the exp ression of a 27 kD p rotein in intercellular fluids of cucumber leaves. This protein was purified by electroelution combined with SDS - PAGE, and analyzed by MALDI-TOF MS. The obtained pep tide mass fingerp rinting ( PMF) was searched in the protein data bank, indicating that it was an acidic chitinase. This was confirmed further by the assay of chitinase activity andWestern blotting.

Key words: cucumber, Pseudoperonospora cubensis, BTH, pathogenesis-related proteins, MALDI-TOF MS, Western blotting

CLC Number:

LI Yu-hong;CHEN Peng;CHENG Zhi-hui;ZHANG Jing;and DONG Jian. Purification and Identification of a Pathogenesis-related Protein in Intercellular Fluids of Cucumber Leaves Induced by BTH[J]. ACTA HORTICULTURAE SINICA, 2009, 36(4): 507-512.

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Purification and Identification of a Pathogenesis-related Protein in Intercellular Fluids of Cucumber Leaves Induced by BTH

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