Cloning of pgip Promoter from Prunus salicina and the Computational Identification of Its Regulatory Element

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (10): 1425-1430.

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Cloning of pgip Promoter from Prunus salicina and the Computational Identification of Its Regulatory Element

LI Guang-ping^1*;ZHANG Chang-qing^3*;ZHANG Zhen^2**;CAO Fu-liang¹

(¹College of Forest Resources and Environm ent, Nanjing Forestry University, Nanjing 210037, China; ²College of Horticulture,Nanjing AgriculturalUniversity, Nanjing 210095, China; ³Department of Horticulture, Jinling Institute of Technology, Nanjing 210038, China)

Received:2009-05-25 Revised:2009-07-13 Online:2009-10-25 Published:2009-10-25
Contact: ZHANG Zhen

Abstract

Abstract: Based on the gene sequence of pgip, we have obtained an up stream fragment of 1 869 bp length by genome walker from Prunus sa licina. Using the bioinformatics methods, including the phylogenetic foot printing and the promoter scanning, we identified three cis2regulatory elements from pgip promoter. One is the TGA1 site and the others areWRKY sites. Our discoveries will be help to understand the transcrip tional regulatory mechanism on pgip expression.

Key words: Prunus salicina, promoter of pgip, clone, regulatory element analysis

CLC Number:

S 662.3

LI Guang-ping;ZHANG Chang-qing;ZHANG Zhen;CAO Fu-liang. Cloning of pgip Promoter from Prunus salicina and the Computational Identification of Its Regulatory Element[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1425-1430.

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Cloning of pgip Promoter from Prunus salicina and the Computational Identification of Its Regulatory Element

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