Abstract:

It aims at the application of mycorrhization technique in the orchid conservation. The endogenetic fungi from the root of the wild plant of Doritis pulcherrima Lindl were inoculated with seedlings of D.pulcherrima in flask and in pot. Firstly, the symbiotic cultivation of aseptic seedlings with the mycelium of solid culture was carried out in flask. According to the method of root coloration, the result showed that the fungi can successfully infect the root of D. pulcherrima seeding after 15d, with 100﹪ survival rate, while that of the control without mycorrhizal fungi was 63.33﹪. After 90 d, the fresh weigh of these seedlings treated with fungi were all higher than the control, especially as for the treatment with fungi F29, there were even 31.3﹪ higher than the control. Secondly, Seedlings were also cultivated with the liquid strain in pot. After 90 d, both the survival rate and the fresh biomass of the mycorrhization seedlings were higher than those of the control. For the mycorrhization seedlings with F29, they have 35﹪ survival rate and 33.24﹪the fresh weigh higher than those of the control, respectively. The results suggested that there was remarkable or significant difference between the inoculated plants and the control.

Key words: orchid, Doritis pulcherrima Lindl., endomycorrhizal fungi, seedling cultivation