Establishment of Genetic Transformation System of Euonymus japonicus 'Cu zhi' Mediated by Agrobacterim tumefaciens

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (3): 409-414.

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Establishment of Genetic Transformation System of Euonymus japonicus 'Cu zhi' Mediated by Agrobacterim tumefaciens

SHANG Ai-qin^1,2, TIAN Chuan-wei¹, ZHAO Liang-jun^1*, and TIAN Ying-chuan³

(¹Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, China; ²College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071001, China; ³Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100080, China)

Received:2007-09-25 Revised:2008-01-10 Online:2008-03-25 Published:2008-03-25
Contact: ZHAO Liang-jun

Abstract

Abstract:

In order to provide genetically improved new germplasm resources and achieve the GNA transgenic plants of Euonymus japonicus 'Cu zhi', genetic transformation system was established based on the system of regeneration. Several factors affected genetic transformation of Euonymus japonicus 'Cu zhi' mediated by Agrobacterium tumefaciens (LBA4404) were studied. The results showed that preculture or not, bacterial concentrateions, the time of infection and co-cultivation had some effect on transformation frequency. The highest transformation frequency was obtained through the following transformation procedure after preculture for 0 d, the explants were infected for 40 min with the concentration of Agrobacterium tumefaciens OD600=0.6 , and then co-cultivated for 3 d. The PCR and PCR-Southern blotting analysis showed the snowdrop lection gene gene was integrated into the genome of Euonymus japonicus 'Cu zhi'. The system of genetic transformation of Euonymus japonicus 'Cu zhi' could provide new germplasm resource used in genetic improvement of Euonymus japonicus 'Cu zhi' and the other plants of Euonymus.

Key words: Euonymus japonicus, snowdrop lectin gene (GNA), genetic transformation

CLC Number:

S 687

SHANG Ai-qin;TIAN Chuan-wei;ZHAO Liang-jun;and TIAN Ying-chuan. Establishment of Genetic Transformation System of Euonymus japonicus 'Cu zhi' Mediated by Agrobacterim tumefaciens [J]. ACTA HORTICULTURAE SINICA, 2008, 35(3): 409-414.

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Establishment of Genetic Transformation System of Euonymus japonicus 'Cu zhi' Mediated by Agrobacterim tumefaciens

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