Isolation and Identification of Four New S-RNase Genes of White Pear( Pyrus bretschneideri)

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 859-864.

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Isolation and Identification of Four New S-RNase Genes of White Pear( Pyrus bretschneideri)

WUYUN Ta-na^1,2；TAN Xiao-feng^1,2；CAO Yu-Fen³；ZHANG Lin^1,2；DENG J ian-jun^1,2

(¹Central-south Forestry University, Key Laboratory of Non-wood Forest Product of Forestry Ministry, Changsha 410004, China;² School of Resources and Environm ent, Central-south Forestry University, Changsha 410004, China; ³ Fruit Tree Research Institute, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract: PCRs were performed on genomic DNAs of four Chinese white pear (Pyrus bretschneideri) culti
varsDonghuang, Xinliyihao, Hongp isu and Boshanchi using degenerate primers derived from conserved amino
acids FTQQYQ and anti-IIWPNV of Japanese pear S_1-8-RNases. Restricted digestion analysis of PCR products
revealed that Xinliyihao and Donghuang had a common S₈
-allele. Sequencing and bioinformatics analysis of un
digested PCR p roduct showed that four alleles from the four cultivarswere distincted from published S₁_-19-alleles
at the DNA and deduced amino acid sequences level, thereby being identified as new alleles. Theywere named
as S₂₀-, S₂₂-, S₂₆- and S₂₇-alleles, respectively ( GenBank accession numbers AY250988, AY250990,
AY339396 and AY339397). Finally, the four cultivarswere S2genotyped as follows: Donghuang (S₈ S₂₀ ) , Xin
liyihao (S₈ S₂₂) , Hongp isu (S₁₂ S₂₆ ) and Boshanchi (S₁₉ S₂₇ ).

Key words: Pyrus bretschneideri, Self-incompatibility, S-allele, S-genotype

WUYUN Ta-na;TAN Xiao-feng;CAO Yu-Fen;ZHANG Lin;DENG J ian-jun;. Isolation and Identification of Four New S-RNase Genes of White Pear( Pyrus bretschneideri)[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 859-864.

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Isolation and Identification of Four New S-RNase Genes of White Pear( Pyrus bretschneideri)

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