Identification of Strawberry Cultivars by RAPD and SCAR Markers

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (3): 591-596.

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Identification of Strawberry Cultivars by RAPD and SCAR Markers

WANG Zhi-gang^1,2；ZHANG Zhi-hong^1*； L IHe¹；GAO Xiu-yan¹；DU Guo-dong¹；TAN Chang-hua¹

( ¹College of Horticulture, Shenyang AgriculturalUniversity, Shenyang 110161, China; ² Institute of Floriculture, Liaoning Academ y of Agricultural Sciences, Shenyang 110161, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

Abstract

Abstract: Thirty-two strawberry cultivarswere tested by randomly amp lified polymorphic DNA (RAPD)
and sequence characterized amp lified region ( SCAR) markers. Results showed that 8 RAPD p rimers p roduced
85 bands, and 71 of them (83。5% ) were polymorphic bands. Among the 32 strawberrymaterials, 25 materi
alswere easy to be distinguished by RAPD markers. The relationship of 28 materialswas analyzed with RAPD
markers, and the synonyms and homonyms cultivars were screened p rimarily. Two dominant SCAR markers
WS01 andWS02 with the length of 378 bp and 214 bp respectively were developed from RAPD markers and
showed the same p rofiles as the corresponding RAPD markers. This study laid a sound foundation for the iden
tification of strawberry cultivar atmolecular level.

Key words: Strawberry, Cultivar identification, RAPD, SCAR

WANG Zhi-gang;ZHANG Zhi-hong;L IHe;GAO Xiu-yan;DU Guo-dong;TAN Chang-hua. Identification of Strawberry Cultivars by RAPD and SCAR Markers[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 591-596.

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Identification of Strawberry Cultivars by RAPD and SCAR Markers

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