Cloning and Characterization of a Full-length cDNA of Pathogenesis-relatedProtein 1 in Brassica rapa ssp. chinensis

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (5): 985-988.

• 研究论文 • Previous Articles Next Articles

Cloning and Characterization of a Full-length cDNA of Pathogenesis-relatedProtein 1 in Brassica rapa ssp. chinensis

Wang Yanhua^{1, 2};Hou Xilin^1*;Shen Shuxing²

(¹National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;²College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071001, China)

Received:2006-02-21 Revised:2006-08-25 Online:2006-10-25 Published:2006-10-25

Abstract

Abstract: In this study, according to the consensus domain of the Brassica napus pathogenesis-related protein PR-1 gene ( accession number U21849) , using RT-PCR and RACE technology, a full-length cDNA of pathogenesis-related protein 1 named BcPR-1 was obtained from Brassica rapa ssp. chinensis cultivar
Suzhouqing, which displayed resistance to downy mildew. Sequence analysis indicated that cloned BcPR-1 gene consisted of 646 nucleotides (nt) containing 161 amino acids. Further comparison to B. napus PR-1 gene and Arabidopsis thaliana PR-1 gene showed that its identitywas 91% and 78% , respectively. Its similarity to other PR-1 genes was 57% to 60%. Southern-blot analysis indicated that there were at least two copies of BcPR-1 gene in B. rapa ssp. chinensis genome. Semi-quantitative RT-PCR analysis revealed that expression of BcPR-1 gene was induced by SA treatment in Suzhouqing, and the corresponding mRNA was accumulated most abundantly 12 h after treatment upon 2 mmol/L SA.

Key words: Brassica rapa ssp. chinensis, Pathogenesis-related protein 1, SA, RT-PCR

Wang Yanhua;;Hou Xilin;Shen Shuxing. Cloning and Characterization of a Full-length cDNA of Pathogenesis-relatedProtein 1 in Brassica rapa ssp. chinensis[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 985-988.

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Cloning and Characterization of a Full-length cDNA of Pathogenesis-relatedProtein 1 in Brassica rapa ssp. chinensis

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