Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine inPummelo

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (5): 1045-1047.

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Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine inPummelo

Yang Xiaoling¹;Cheng Zhou¹;Li Shan¹;Kitajima Akira²;Hasegawa Kojiro³

(¹School of Life Science and Technology, Tongji University, Shanghai 200092, China; ² Experimental Farm, Kyoto University,Osaka 56920096, Japan; ³Faculty of Agriculture, Kochi University, Kochi 78328502, Japan)

Received:2005-11-06 Revised:2006-02-23 Online:2006-10-25 Published:2006-10-25

Abstract

Abstract: Tosa-Buntan pummelo 〔Citrus grandis (L. ) Osb. 〕was used to establish a method of 2n gametes induced by colchicine. The effects of colchicine concentration and its treatment period on the derived self-hybridized triploids were also analyzed. The results showed that 2n gametes with self-compatibility could be induced by treating the bud with 0.25% colchicine at 1 week before flowering. 25% of the alive F₁ plants were considered as the self-hybridized trip loids after the measurement of leave cellular nucleus DNA content and cytological identification. Therefore, it is the effective method to obtain the self-hybridized triploids derived from 2n gametes induced by colchicines in pummelo.

Key words: Pummelo, Colchicine, 2n gamete, Triploid

CLC Number:

S 666.3

Yang Xiaoling;Cheng Zhou;Li Shan;Kitajima Akira;Hasegawa Kojiro. Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine inPummelo[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 1045-1047.

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Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine inPummelo

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