Dentification of Three Species in Bauhinia and Hybrid Origin of Bauhinia blakeana Using ISSR Markers

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 433-436.

• 研究简报 • Previous Articles Next Articles

Dentification of Three Species in Bauhinia and Hybrid Origin of Bauhinia blakeana Using ISSR Markers

Luo Yuping¹;GongWei¹;Qiu Yingxiong^1*;Yuk Sing Gibert Chan^2*

(¹Laboratory of Systematic and Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou 310029, China; ²Department of Applied Biology and Chemical Technology, the Hongkong Polytechnic University, Hung Hom, Hongkong, China)

Received:2005-06-01 Revised:2005-08-09 Online:2006-04-25 Published:2006-04-25

Abstract

Abstract: In this study, inter-simple sequence repeat (ISSR) was evaluated for its potential use in the identification of Bauhinia purpurea, B. blakeana and B. variegate. Of the 74 ISSR primers subjected to screening, 9 produced reproducible, polymorphic and discrepant fragments. Using these primers, 80 discernible DNA fragmentswere generated with 55 (68.8% ) being polymorphic bands. The average number of DNA bands amplified by each primer was 8.88. DNA profiles based on 8 primers have revealed potential diagnostic fingerprints which could contribute to the identification of 3 species in Bauhinia. Meanwhile, ISSR - PCR analysis revealed that the amplified fragments respectively unique to B. purpurea and B. variegate showed additivity in B. blakeana. The molecular evidence indicated that B. blakeana was a hybrid origin from B. purpurea and B. variegate.

Key words: Bauhinia, B. blakeana, B. purpurea, B. variegate, ISSR, DNA fingerprint

Luo Yuping;GongWei;Qiu Yingxiong;Yuk Sing Gibert Chan. Dentification of Three Species in Bauhinia and Hybrid Origin of Bauhinia blakeana Using ISSR Markers[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 433-436.

[1]	WANG Xin, LI Mingyang, TIAN Lin, LIU Dongyun. ISSR and rDNA-ITS Sequence Analysis of the Genetic Relationship of Clematis in Hebei Province [J]. Acta Horticulturae Sinica, 2021, 48(9): 1755-1767.
[2]	YU Bo1，HUANG Lili1，ZHU Yu2，ZHU Genfa1，and SUN Yingbo1,*. Induction of Embryogenic Calli from Immature Pedicels and Efficient Plant Regeneration of Hippeastrum [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 907-915.
[3]	BEN Haiyan1,3，QU Hongyun1，GAO Wei3，ZHANG Junmin1，ZHANG Xueyan1，LI Baoju2,，and CHAI Ali2,. Pathogenicity Differentiation and ISSR Genetic Variation Analysis of Verticillium dahliae Isolates from Eggplant in Northeast China [J]. ACTA HORTICULTURAE SINICA, 2019, 46(7): 1320-1332.
[4]	SUN Hongmei1,2,*，FU Linlan1，WANG Zhiping1，GAI Meizhu1，and WANG Chunxia1,2. Polyploidy Induction and Identification of Lilium pumilum and Lilium davidii var. unicolor Based on Somatic Embryogenesis [J]. ACTA HORTICULTURAE SINICA, 2018, 45(6): 1136-1146.
[5]	JIANG Yawen，SUN Xiaoqin，LUO Huolin，YANG Boyun，and XIONG Dongjin. Studies on Genetic Diversity of Cymbidium kanran Populations from the Main Mountains in Jiangxi Province Based on ISSR Marker [J]. ACTA HORTICULTURAE SINICA, 2017, 44(10): 1993-2001.
[6]	CHEN Zongyou1,2,3，HUANG Xiyang2，TANG Hui1,2,，WANG Manlian2，and CHAI Shengfeng1,2. ISSR Analysis on Genetic Diversity for Germplasm Resources of Rubus* suavissimus [J]. ACTA HORTICULTURAE SINICA, 2017, 44(1): 161-169.
[7]	. SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars [J]. ACTA HORTICULTURAE SINICA, 2016, 43(11): 2266-2276.
[8]	LI Xue-ping1，GUO Song2,3，XIONG Jun-fei1,3，GUO Hua-wei1，ZHENG Lin1，and LI Zai-liu3,*. Genetic Diversity of the Endangered Handeliodendron bodinieri in Guangxi Province by ISSR and SRAP Analysis [J]. ACTA HORTICULTURAE SINICA, 2015, 42(2): 386-394.
[9]	DONG Hai-yan1,，JI Kong-shu2，HOU Bo-xin3，and ZHAO Hong-bo1. Genetic Relatives Analysis of 41 Loropetalum chinense* var. rubrum Cultivars by ISSR Markers [J]. ACTA HORTICULTURAE SINICA, 2014, 41(2): 365-374.
[10]	ZHOU Kun-Hua, CHEN Xue-Jun, FANG Rong, CHEN Li-Zhen, ZONG Hong-Xia, MIAO 南Sheng. Construction and Analysis of an Interspecific Linkage Map of Capsicum annuum × C. frutescens [J]. ACTA HORTICULTURAE SINICA, 2013, 40(11): 2171-2179.
[11]	LIN Li, HU Zhong-Yi, LI Ji-Yuan, ZHU Zhi-Yong, NI Sui. Analysis on Genetic Diversity of Ten Insular Populations of Camellia japonica [J]. ACTA HORTICULTURAE SINICA, 2012, 39(8): 1531-.
[12]	MENG Jin-Gui, ZHANG Qing-Zhe, WANG Shuo, ZHANG Ying-Hua. Studies on the Genetic Relationship Between Capsicum frutescens var. shuanlaense and Other Five Cultivated Capsicum Species [J]. ACTA HORTICULTURAE SINICA, 2012, 39(8): 1589-.
[13]	WEI Rong-Chang, LI Hong, JIANG Jian-Gang, LI Feng, JIANG Shui-Yuan, HUANG Xi-Yang. ISSR Analysis of Genetic Background of Polyploidy Seedless Siraitia grosvenorii and Their Parents [J]. ACTA HORTICULTURAE SINICA, 2012, 39(2): 387-394.
[14]	SHI Yan-Tong, ZHOU Bo, ZHANG Xiu-Xin, JIANG Hai-Dong, XUE Jing-Qi, WANG Shun-Li. Assessment of Genetic Diversity and Relationship of 89 Tree Peony Cultivars from Different Provenances [J]. ACTA HORTICULTURAE SINICA, 2012, 39(12): 2499-2506.
[15]	HE Zhi-Jiao, HE Jia-Wei, CHENG Zai-Quan, YANG Zheng-Song, YANG Hong-Tao, HE Wen-Jia. Genetic Diversity in the Natural Populations of Rubus delavayi Demonstrated by Inter-simple Sequence Repeats [J]. ACTA HORTICULTURAE SINICA, 2012, 39(11): 2142-2150.

Dentification of Three Species in Bauhinia and Hybrid Origin of Bauhinia blakeana Using ISSR Markers

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments