The AFLP Markers Linked with the Bitter Fruit Gene ( Bt) in Cucumber

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (1): 140-142.

• 研究简报 • Previous Articles Next Articles

The AFLP Markers Linked with the Bitter Fruit Gene ( Bt) in Cucumber

Gu Xingfang¹;Zhang Suqin^{1, 2};and Zhang Shengping¹

(¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China)

Received:2005-06-30 Revised:2005-07-28 Online:2006-02-25 Published:2006-02-25

Abstract

Abstract: AFLP biotechnology was applied to identify the markers linked to Bt gene based on BSA method. Two dominant AFLP markers E23M662101 and E25M652213 were screened firstly in home and abroad. The bitter fruit F₂ plants possessed 101 bp and 213 bp fragments, but the bitterfree F₂plants not. The genetic distance of E23M662101 and E25M652213 was 5 cM and 4 cM respectively, and two markerswere two sides of Bt gene. The AFLP markers can be used for marker-assisted selection.

Key words: Cucumber, Bt gene, AFLP marker

Gu Xingfang;Zhang Suqin;;and Zhang Shengping. The AFLP Markers Linked with the Bitter Fruit Gene ( Bt) in Cucumber[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 140-142.

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The AFLP Markers Linked with the Bitter Fruit Gene ( Bt) in Cucumber

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