RAPD Studies on Sport Line of‘Shixia’Longan with Double Primers

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (1): 134-136.

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^{RAPD Studies on Sport Line of‘Shixia’Longan with Double Primers}

Wang Xinyan¹;Xiao Xuan²;Qiao Aimin¹; and Sun Min³

(¹Departm ent of Horticulture, Zhongkai University of Agriculture and Technology, Guangzhou 510225, China; ²Department of Chemistry and Life Science, Gannan Normal University, Ganzhou 341000, China; ³College of Life Science, Southwest China Normal University, Chongqing 400715, China)

Received:2005-01-25 Revised:2005-07-22 Online:2006-02-25 Published:2006-02-25

Abstract

Abstract: A single 10 bp random oligonucleotide was often used as the primer in the standard RAPD-PCR analysis. To identify the possibility of RAPD-PCR reaction with double 10 bp primers, 80 double 10 bp oligonucleotideswere applied to the RAPD-PCR analysis between the ‘Shixia’ longan (Dimocarpus longan var. shixia Lour. ) sport line and itps normal line ( control). Four double primers which could produce clear and stability polymorphic DNA fragments were screened. Five RAPD makers have been obtained which were linked to the mutation gene of the sport line. Furthermore, the reason why the new RAPD fragments could be produced by double primers was also discussed.

Key words: Longan, Sport, RAPD, Double primers

Wang Xinyan;Xiao Xuan;Qiao Aimin;and Sun Min. ^{RAPD Studies on Sport Line of‘Shixia’Longan with Double Primers}[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 134-136.

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^{RAPD Studies on Sport Line of‘Shixia’Longan with Double Primers}

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