Studies on Transferring P5CS Gene into Tall Fescue ( Festuca arundinaceaSchreb. ) via Microprojectile Bombardment

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (4): 853-657.

• 研究论文 • Previous Articles

Studies on Transferring P5CS Gene into Tall Fescue ( Festuca arundinaceaSchreb. ) via Microprojectile Bombardment

Li Zhiliang1, 3;Huang Conglin1;Zhang Xiuhai1;CaoMingqing1;Li Zheng2;Wang Gang3;Wu Zhongyi

(¹Beijing Research Center of Agro-Biotechnology, Beijing 100089, China; ²Beijing Research and Development Center for Grass and Environment, Beijing 100089, China; ³College of Life Science Hebei Normal University, Shijiazhuang 050016, China)

Received:2004-09-11 Revised:2005-01-17 Online:2005-08-25 Published:2005-08-25

Abstract

Abstract: In this study, a mutated form of V igna aconitifolia Δ1-pyrroline-5-carboxylate synthetase( P5CS -F129A ) gene was transferred into calli of tall fescue by micro p rojectile bombardment. Twenty2five transgenic p lants were obtained through phosphinothricin screening, PCR and Southern blot analysis. The
transformation frequency in calli of tall fescue was about 1.4%. The content of proline in transgenic plants was 31% - 83% higher than that in untransformed plants. Furthermore, the transgenic p lants were more tolerant to drought stress.

Key words: Turfgrass, Tall fescue, Transgenic, Micro projectile bombardment, Drought tolerance

CLC Number:

S 68

Li Zhiliang;;Huang Conglin;Zhang Xiuhai;CaoMingqing;Li Zheng;Wang Gang;Wu Zhongyi. Studies on Transferring P5CS Gene into Tall Fescue ( Festuca arundinaceaSchreb. ) via Microprojectile Bombardment[J]. ACTA HORTICULTURAE SINICA, 2005, 32(4): 853-657.

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Studies on Transferring P5CS Gene into Tall Fescue ( Festuca arundinaceaSchreb. ) via Microprojectile Bombardment

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