Estabilishnent of Embryo Culture Technical System of Chinese Jujube ( Zizyphus jujuba Mill. )

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (3): 496-499.

• 研究报告 • Previous Articles Next Articles

Estabilishnent of Embryo Culture Technical System of Chinese Jujube ( Zizyphus jujuba Mill. )

Du Xuemei¹;Li Dengke¹;Wang Yongkang¹;Sui Chuanling¹;Fan Baoguo²

(¹ Institute of Pomology, Shanxi Academy of Agricultural Sciences, Taigu 030815, China; ²Shanxi Normal Unirersity, Linfen 041000, China)

Received:2004-08-12 Revised:2005-01-11 Online:2005-06-25 Published:2005-06-25

Abstract

Abstract: The factors affecting embryo culture were studied in Zizyphus jujuba ‘Liuyuexian’. The youngest embryo saved successfullywas 50 days old. For the forming embryos, the op timal concentration of sucrose was 7% , for the formed ones was 5%. Dark treatment is beneficial to embryo sprouting, growing and rooting. There are extremely significance effects of plant growth regulators and organic substance on embryo culture. The media of 1 /2MS + IBA 0.4 (mg/L, the same below ) +BA 0.8 +LH 500 + GA₃ 5 + 5%
sucrose is suitable for culture of 70 - 72 days old embryo. The rootless seedling should be transferred to 1 /2 MS + IBA 1.0 + IAA 0.06 + 2% sucrose to induce normal root system.

Key words: Chinese jujube, Embryo, Tissue culture

CLC Number:

S 665.1

Du Xuemei;Li Dengke;Wang Yongkang;Sui Chuanling;Fan Baoguo. Estabilishnent of Embryo Culture Technical System of Chinese Jujube ( Zizyphus jujuba Mill. )[J]. ACTA HORTICULTURAE SINICA, 2005, 32(3): 496-499.

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Estabilishnent of Embryo Culture Technical System of Chinese Jujube ( Zizyphus jujuba Mill. )

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