Dodecaploid Plantlets Regenerated from Endosperm Culture of ‘Luotiantianshi’Persimmon

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 589-592.

• 研究论文 • Previous Articles Next Articles

Dodecaploid Plantlets Regenerated from Endosperm Culture of ‘Luotiantianshi’Persimmon

Chen Xuzhong;Luo Zhengrong^*

( College of Horticulture and Forestry , Huazhong Agricultural University , Wuhan 430070 , China)

Received:2003-10-14 Revised:2004-02-20 Online:2004-10-25 Published:2004-10-25

Abstract

Abstract: Endosperms of‘Luotiantianshi’persimmon ( Diospyros kaki Thunb. ) were cultured with after different auxin and cytokinin concent ration combination open pollination 70 days. Result s showed that the 1/ 2MS (1/ 2N) medium contained 2,4-D 1.0 mg/ L + ZT 1.1 mg/ L + CH 1000 mg/ L was better for
inducing callus to from regenerated plantlets , Stomatal characteristics、flow cytometric analysis and chromosome counting proved dodecaploid (2n=12x= 180) presence in those regenerated plantlets. The regenerated dodecaploid plants could be used as parental material in breeding program.

Key words: Persimmon, Nonast ringent persimmon, Endosperm culture, Polyploid, Dodecaploid, Regenerated plantlet

CLC Number:

S 665.2

Chen Xuzhong;Luo Zhengrong. Dodecaploid Plantlets Regenerated from Endosperm Culture of ‘Luotiantianshi’Persimmon[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 589-592.

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Dodecaploid Plantlets Regenerated from Endosperm Culture of ‘Luotiantianshi’Persimmon

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