Abstract: The main factors of crowd shoot induction，propagation and rooting of Oncidium were studied by using different growth period of bud．The results showed that the primary bud is better explants than others．The different kinds and different concentration of hormones greatly affected crowd shoot induction，propagation and rooting of Oncidium．The suitable culture medium for crowd shoot induction is MS+6-BA 4.0 mg/L+NAA 0.5 mg/L +3%sucrose． The best culture medium for shoot propagation is MS+ 6-BA 2.0~4.0 mg/L+NAA 0.5 mg/L+3% sucrose．The proper culture medium for rooting is 1/2 MS+IBA 0.5 mg/L +2% sucrose．

Key words: Oncidium, Tissue culture, Cytokinin, Auxin