Immunity Coloidal Gold Localization of Calmodulin in Cucumber Young LeafCells

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (6): 728-730.

• 研究报告 • Previous Articles Next Articles

Immunity Coloidal Gold Localization of Calmodulin in Cucumber Young LeafCells

Tian Jinghua，Zhang Hang，Li Ming， and Wang Mei

(College ofHorticulture，Hebei Agricultural University，Baoding 071001， China)

Received:2002-10-09 Revised:2002-12-06 Online:2003-12-25 Published:2003-12-25

Abstract

Abstract: Using glutaraldehyde an d parafonnaldehyde as fixative an d Lowicryl K4M as embedding medium，the immunity colloidal gold localization of calmodulin (CaM ) in cucunber young leaf cells Was investigated by transmission electron mi croscope． The results were as follows： at the optimal growth temperature， CaM mainly distributed in nucleus an d chloroplast． In addition， there Was some CaM in mitoehondria， a little in cytoplasm， vac
uole，tonoplast an d plasma membrane． However， it Was difficult to find CaM in cell wall an d intercelular space．

Key words: Cucumber, Calnlodulin, Immunity colloidal gold, Transmi ssion electron microscope

CLC Number:

Tian Jinghua;Zhang Hang;Li Ming;and Wang Mei. Immunity Coloidal Gold Localization of Calmodulin in Cucumber Young LeafCells[J]. ACTA HORTICULTURAE SINICA, 2003, 30(6): 728-730.

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Immunity Coloidal Gold Localization of Calmodulin in Cucumber Young LeafCells

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