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ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (1): 56-58.

• 研究论文 • Previous Articles     Next Articles

Cloning and Characterization of SRK-Binding Protein THL1 Gene from Brassica oleracea L. in Self-incompatibility Signaling Process

Liu Dong1;Zhu Liquan1;Wang Xiaojia2   

  1. (1Laboratory of Biochemistry , Department of Agronomy and Bioscience ,2 Chongqing Key Laboratory of Olericulture , Southwest Agricultural University , Chongqing 400716 , China)
  • Received:2002-04-20 Revised:2002-08-02 Online:2003-02-25 Published:2003-02-25

Abstract: The THL1 gene and it’s cDNA coding for thioredoxin-like protein were cloned from genomic DNA and stigma mRNA in Brassica oleracea L. by PCR and RT-PCR respectively. Their sizes are 719 bp and 430 bp respectively. Sequencing result showed that this gene contained two introns and could specify a peptide with 117 amino acid sequence.

Key words: Brassica oleracea L., Thioredoxin-like protein THL1, Self-incompatibility

CLC Number: