Main Factors Influencing the Frequency of Direct Organogenesis of Cucumberin Vitro

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (1): 101-103.

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Main Factors Influencing the Frequency of Direct Organogenesis of Cucumberin Vitro

Hou Aiju;Zhu Yanming;Yang Aifu;Zhang Binbin

( Plant Bioengineering Laboratory , Life Science Institute, North East Agriculture University , Harbin 150030 , China)

Received:2002-02-07 Revised:2002-06-17 Online:2003-02-25 Published:2003-02-25

Abstract

Abstract: The main factors influencing the frequency of direct organogenesis of cucumber ( Cucumis sativus L. ) were discussed. The results showed that the types of explants , genotypes and plant growth regulator had significantly effects on regenerating frequency. The optimum explant is cotyledonary nodes. Genotypes ‘Changchun mici’and ‘Longzahuang 7’had higher frequency of regeneration which were 93. 2 % and 92. 1 % respectively. But the former differentiated much more buds (13. 2 per explant) than that of the latter (3. 1 per explant) . 6-BA can effectively induced buds and the optimum concentration is 0. 5 mg/ L.

Key words: Cucumber, High frequency regenerated system, Direct organogenesis, Differentiation rate of buds

CLC Number:

S 642. 2

Hou Aiju;Zhu Yanming;Yang Aifu;Zhang Binbin. Main Factors Influencing the Frequency of Direct Organogenesis of Cucumberin Vitro[J]. ACTA HORTICULTURAE SINICA, 2003, 30(1): 101-103.

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Main Factors Influencing the Frequency of Direct Organogenesis of Cucumberin Vitro

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