Cloning of a cDNA Fragment of Acid lnvertase Gene from Cucumis melo L．Fruit

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (03): 346-348.

• 研究报告 • Previous Articles Next Articles

Cloning of a cDNA Fragment of Acid lnvertase Gene from Cucumis melo L．Fruit

Yu Xiyan¹; Zhao Shuangyi²; He Qiwei³; Kong Qingguo⁴

( ¹School of Horticulture Shandong Agricultural University, Tai'an 271018, China; ²School of Sciences，Shandong University, Ji'nan 250100，China; ³Vegetable Institute，Shandong Academy of Agricultural Sciences，Ji'nan 250100，China; ⁴Binzhou Vocational College of Shandong Province，Binzhou 256624，China)

Received:2002-07-16 Revised:2002-11-26 Online:2003-06-25 Published:2003-06-25

Abstract

Abstract: PCR primers were designed based on the eonsensu$ domain of some acid invertase genes in GenBank．The aimed cDNA fragment was amplified from the total RNA isolated from melon fruit via RT-PCR and cloned into pMD18-T vector. The amino acid sequence determined by this fragment shows 99％ homology to that of the corresponding region of acid invertase of tomato. This confirms that we have cloned a cDNA fragment from melon fruit successfully. the access number of this gene in GenBank is AF490425.

Key words: Melon, Fruit, RT—PCR, Acid invertase gene

CLC Number:

S 652

Yu Xiyan;Zhao Shuangyi;He Qiwei;Kong Qingguo. Cloning of a cDNA Fragment of Acid lnvertase Gene from Cucumis melo L．Fruit[J]. ACTA HORTICULTURAE SINICA, 2003, 30(03): 346-348.

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Cloning of a cDNA Fragment of Acid lnvertase Gene from Cucumis melo L．Fruit

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