High Efficient System Establishment of Plant Regengation from CotyledonExplants of Pepper ( Capsicum annuum L. )

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (1): 25-29.

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High Efficient System Establishment of Plant Regengation from CotyledonExplants of Pepper ( Capsicum annuum L. )

Li Dingjun^{1 ,2} , Zhang Baoxi¹ , Zhao Kaijun¹ , Xie Bingyan¹ , and Luo Kuan²

(¹ Institute of Vegetables and Flowers , Chinese Academy of Agricultural Science , Beijing 100081;　² Department of Plant Protection , Hunan Agricultural University , Changsha 410128)

Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

Abstract

Abstract: The report describes the development of a high efficient in vitro regeneration procedure from cotyledon explants of 17 sweet/ chilli pepper cultivars (Shuangfeng et al) . The effect of genotype , hormone , organic nutrient component and AgNO3 et al on the tissue culture from pepper cotyledon explants was investigated. Adding DJ(juice of peppr stem and leaf + kanamycin = 500∶1) organic nutrient component can improved the shoot buds differentiation and elongation rate. The best media for in vitro regeneration from cotyledon explants of pepper were discovered : the shoot buds induction medium: MB + IAA 1. 0 mg/ L + 62BA 5. 0 mg/ L + DJ 5. 0 g/ L + AgNO3 10. 0
mg/ L ; the shoot buds elongation medium: MB + IAA 1. 0 mg/ L + 62BA 5. 0 mg/ L + DJ 5. 0 g/ L + AgNO3 10. 0 mg/ L + GA32. 0 mg/ L ; the rooting medium: MS + IAA 1. 0 mg/ L + NAA 0. 1 mg/ L.

Key words: Pepper, Cotyledon, Plant regeneration

CLC Number:

S 643. 1

Li Dingjun;;Zhang Baoxi;Zhao Kaijun;Xie Bingyan;and Luo Kuan. High Efficient System Establishment of Plant Regengation from CotyledonExplants of Pepper ( Capsicum annuum L. )[J]. ACTA HORTICULTURAE SINICA, 2002, 29(1): 25-29.

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High Efficient System Establishment of Plant Regengation from CotyledonExplants of Pepper ( Capsicum annuum L. )

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