Regeneration of Peach Plantlet from Callus Derived from Explant

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (4): 342-344.

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Regeneration of Peach Plantlet from Callus Derived from Explant

Zhang Yongqing¹ , Chen Daming¹, Jin Yongfeng² , and Zhang Shanglong¹

(¹ Department of Horticulture , Zhejiang University , Hangzhou 310029;　² Institute of Biochemistry , Zhejiang University , Hangzhou 310029)

Received:1900-01-01 Revised:1900-01-01 Online:2001-08-25 Published:2001-08-25

Abstract

Abstract: Various explants of ‘Yulu’peach including leave , young shoot , hypocotyl , ovule , ovary , endosperm, immature embryo collected approximately 45 - 50 days or 75 days after full bloom , and mature embryo were cultured in vitro. As a result , only the white nodular callus derived from immature embryos collected 45 - 50 days and 75 days after bloom succeeded in producing adventitious buds , and the regeneration rates of white callus were 73. 8 %
and 15. 5 % respectively. 62BA had stronger effect than KT in inducing adventitious bud formation. Adventitious shoots were transferred and cultured to plantlets. Effect of different hormone , carbon source , medium and transfer time on redifferentiation of adventitious buds from callus derived from immature embryos have been discussed.

Key words: Peach ( Prunus persica), Explant, Callus, Redifferentiation, Plantlet, Tissue culture

CLC Number:

Zhang Yongqing;Chen Daming;Jin Yongfeng;and Zhang Shanglong. Regeneration of Peach Plantlet from Callus Derived from Explant [J]. ACTA HORTICULTURAE SINICA, 2001, 28(4): 342-344.

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Regeneration of Peach Plantlet from Callus Derived from Explant

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