Arbuscular Mycorrhizal Fungi-Specific Lipid Allocation Patterns in Citrus Mycorrhizae in Response to Drought Stress

doi:10.16420/j.issn.0513-353x.2025-0960

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (4): 1013-1024.doi: 10.16420/j.issn.0513-353x.2025-0960

• Research on Arbuscular Mycorrhizal Fungi in Horticultural Plants • Previous Articles Next Articles

Arbuscular Mycorrhizal Fungi-Specific Lipid Allocation Patterns in Citrus Mycorrhizae in Response to Drought Stress

ZHANG Wei¹, YIN Xilong¹, FENG Zengwei², LIU Xiaodi², ZHOU Yang², ZHU Honghui², YAO Qing¹^,^*()

¹ College of Horticulture，South China Agricultural University，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（South China），Ministry of Agriculture and Rural Affairs，Guangdong Provincial Key Laboratory of Microbial Signaling and Disease Control，Guangdong Engineering Research Center for Litchi，Guangzhou 510642，China
² Institute of Microbiology，Guangdong Academy of Sciences，Guangdong Academy of Sciences，State Key Laboratory of Applied Microbiology Southern China，Key Laboratory of Agricultural Microbiomics and Precision Application（MARA），Key Laboratory of Agricultural Microbiome（MARA），Guangzhou 510075，China

Received:2025-10-14 Revised:2026-03-10 Online:2026-04-25 Published:2026-04-20
Contact: YAO Qing

Abstract

Abstract:

With trifoliate orange[Poncirus trifoliata（L.）Raf.]as plant materials inoculated with arbuscular mycorrhizal fungus（AMF）Rhizophagus irregularis DAOM197198 or not，this study investigated the allocation patterns of arbuscular mycorrhizal fungi（AMF）-specific lipids in response to drought stress under well watered（soil water content 18%）or drought stressed（soil water content 10%）conditions，in which the lipid contents in roots and soils and the expression levels of related genes were determined. Results showed that AMF significantly promoted plant growth，with the biomass increasing by 49.09% and 29.76% respectively after 1（weak stress）and 4（strong stress）weeks of drought. Drought significantly inhibited AMF colonization，and significantly reduced the AMF-specific lipid（C16∶1ω5）content as well. The specific phospholipid fatty acid（PLFA-C16∶1ω5）contents decreased by 18.59% and 61.52% respectively after 1（weak stress）and 4（strong stress）weeks of drought，while the specific neutral lipid fatty acid（NLFA-C16∶1ω5）contents decreased by 54.08% and 46.05%，indicating the greater effect of strong stress on PLFA-C16∶1ω5 than that of weak stress. Under well watered condition，NLFA-C16∶1ω5 mainly distributed intraradically with drought promoting it to distribute outwards in soils，while PLFA-C16∶1ω5 mainly distributed extraradically with drought further increasing its proportion in soils. Moreover，drought inhibited the expression of genes related to specific lipid synthesis and transport（PtFatM，PtRAM2，PtSTR，PtSTR2），P transporter PtPT4 and sugar transporter PtSWEET2. In conclusion，considering the close relations of PLFA-C16∶1ω5 with arbuscule（hyphae），and NLFA-C16∶1ω5 with spores（vesicles），it is acceptable that AMF respond to drought stress by differentially allocating lipids based on function（PLFA vs. NLFA）and spatial distribution（intraradical vs. extraradical）.

Key words: Poncirus trifoliata, drought stress, arbuscular mycorrhizal fungi, AMF-specific fatty acids, C16∶1ω5, lipid allocation

ZHANG Wei, YIN Xilong, FENG Zengwei, LIU Xiaodi, ZHOU Yang, ZHU Honghui, YAO Qing. Arbuscular Mycorrhizal Fungi-Specific Lipid Allocation Patterns in Citrus Mycorrhizae in Response to Drought Stress[J]. Acta Horticulturae Sinica, 2026, 53(4): 1013-1024.

Figures/Tables 7

References 33

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基因 Gene	引物序列（5′-3′） Primer sequence	扩增片段大小/bp Amplification fragment size
Actin	F：CCGACCGTATGAGCAAGGAAA R：TTCCTGTGGACAATGGATGGA	190
PtFatM	F：CCGGTCTTCTCAGCAATGGT R：TTCAACAACCTCTCCCCAGA	122
PtRAM2	F：GCTGATTTGGTTCTGGGCAC R：ACCAAGTAACCCTCCTTGCAC	145
PtSTR	F：ACTGGGTTTACGGAGTGCAA R：TCAAGGAACAACAGCGACGG	76
PtSTR2	F：TCGCCGCTGATTTTCGTTTG R：TACGTGTTCCGAGTTGTCGTC	103
PtPT4	F：TCGCTGCAAGTACAGGAAAGA R：ACTGTCAGTTGATCTCCAGCC	73
PtSWEET2	F：TATGGCACGCCCCTTGTATC R：CCAAGCATCCGCACCTTTTT	134

基因 Gene	引物序列（5′-3′） Primer sequence	扩增片段大小/bp Amplification fragment size
Actin	F：CCGACCGTATGAGCAAGGAAA R：TTCCTGTGGACAATGGATGGA	190
PtFatM	F：CCGGTCTTCTCAGCAATGGT R：TTCAACAACCTCTCCCCAGA	122
PtRAM2	F：GCTGATTTGGTTCTGGGCAC R：ACCAAGTAACCCTCCTTGCAC	145
PtSTR	F：ACTGGGTTTACGGAGTGCAA R：TCAAGGAACAACAGCGACGG	76
PtSTR2	F：TCGCCGCTGATTTTCGTTTG R：TACGTGTTCCGAGTTGTCGTC	103
PtPT4	F：TCGCTGCAAGTACAGGAAAGA R：ACTGTCAGTTGATCTCCAGCC	73
PtSWEET2	F：TATGGCACGCCCCTTGTATC R：CCAAGCATCCGCACCTTTTT	134

处理 Treatment	1周 1 Week			4周 4 Weeks
处理 Treatment	NLFA-C16:1ω5/ （nmol · g^-1）	PLFA-C16:1ω5/ （nmol · g^-1）	NLFA-C16:1ω5/ PLFA-C16:1ω5	NLFA-C16:1ω5/ （nmol · g^-1）	PLFA-C16:1ω5/ （nmol · g^-1）	NLFA-C16:1ω5/ PLFA-C16:1ω5
正常Well-watered	215.51 ± 15.25^**	106.16 ± 7.85^NS	2.03 ± 0.11^*	158.89 ± 12.55^**	185.09 ± 33.87^**	0.86 ± 0.15^NS
干旱Drought stress	98.97 ± 17.65	86.42 ± 9.59	1.15 ± 0.29	85.72 ± 12.47	71.21 ± 6.71	1.20 ± 0.18

处理 Treatment	1周 1 Week			4周 4 Weeks
处理 Treatment	NLFA-C16:1ω5/ （nmol · g^-1）	PLFA-C16:1ω5/ （nmol · g^-1）	NLFA-C16:1ω5/ PLFA-C16:1ω5	NLFA-C16:1ω5/ （nmol · g^-1）	PLFA-C16:1ω5/ （nmol · g^-1）	NLFA-C16:1ω5/ PLFA-C16:1ω5
正常Well-watered	215.51 ± 15.25^**	106.16 ± 7.85^NS	2.03 ± 0.11^*	158.89 ± 12.55^**	185.09 ± 33.87^**	0.86 ± 0.15^NS
干旱Drought stress	98.97 ± 17.65	86.42 ± 9.59	1.15 ± 0.29	85.72 ± 12.47	71.21 ± 6.71	1.20 ± 0.18

处理 Treatment	1周 1 Week				4周 4 Weeks
处理 Treatment	NLFA-C16:1ω5/ （nmol · g^-1）	占比/% Proprotion	PLFA-C16:1ω5/ （nmol · g^-1）	占比/% Proprotion	NLFA-C16:1ω5 （nmol · g^-1）	占比/% Proprotion	PLFA-C16:1ω5/ （nmol · g^-1）	占比/% Proprotion
正常Well-watered	—	—	306.66 ± 33.74^NS	100.0	200.38 ± 6.22^*	31.4	437.61 ± 21.35^**	68.6
干旱Drought stress	66.17 ± 41.46	17.7	307.97 ± 23.85	82.3	106.98 ± 41.24	27.3	285.57 ± 27.44	72.7

Arbuscular Mycorrhizal Fungi-Specific Lipid Allocation Patterns in Citrus Mycorrhizae in Response to Drought Stress

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