Screening of ABCG Genes Related to the Transport of Volatile Compounds in Chimonanthus praecox

doi:10.16420/j.issn.0513-353x.2025-0926

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (2): 613-634.doi: 10.16420/j.issn.0513-353x.2025-0926

• Research Papers • Previous Articles

Screening of ABCG Genes Related to the Transport of Volatile Compounds in Chimonanthus praecox

LIU Yuting, FU Xuemei, HU Yihang, CHEN Longqing()

College of Landscape Architecture and Horticulture Sciences，Southwest Forestry University，Kunming 650224，China

Received:2025-12-14 Revised:2026-01-09 Online:2026-02-25 Published:2026-02-12

Abstract

Abstract:

Fourty-four CpABCG genes were identified in Chimonanthus praecox through bioinformatic analysis. Characterization of the ABCG subfamily and its potential role in floral scent emission was preliminarily investigated. All CpABCG proteins contain transmembrane domains（TMD）and nucleotide-binding domains（NBD）；The analysis of conserved motif revealed that Motif 1，3，and 4 are characteristic of the ABCG family. The collinearity analysis indicated closer phylogenetic relationship between CpABCG and PmABCG；The promoters of CpABCG genes contain cis-elements responsive to light，plant development，abiotic stress，and phytohormones；The results of qRT-PCR indicated that three genes CpABCG1.2，CpABCG9.1 and CpABCG25.2 are highly expressed in flowers，and their expression patterns are closely related to the release pattern of floral fragrance in C. praecox；After the treatment with exogenous floral scent inhibitors，the total floral emission decreased significantly，while the endogenous volatile substances increased correspondingly；Based on expression profiling，CpABCG1.2 and CpABCG9.1 were proposed to play key roles in transmembrane transport of floral volatile compounds in C. praecox.

Key words: Chimonanthus praecox, ABCG transporter, floral fragrance, transmembrane transport

LIU Yuting, FU Xuemei, HU Yihang, CHEN Longqing. Screening of ABCG Genes Related to the Transport of Volatile Compounds in Chimonanthus praecox[J]. Acta Horticulturae Sinica, 2026, 53(2): 613-634.

Figures/Tables 18

References 48

[1]	Adebesin F, Widhalm J R, Boachon B, Lefevre F, Pierman B, Lynch J H, Alam I, Junqueira B, Benke R, Ray S. 2017. Emission of volatile organic compounds from petunia flowers is facilitated by an ABC transporter. Science, 356:1386-1388. doi: 10.1126/science.aan0826 pmid: 28663500
[2]	Bird D, Beisson F, Brigham A, Shin J, Greer S, Jetter R, Kunst L, Wu X, Yephremov A, Samuels L. 2007. Characterization of Arabidopsis ABCG11/WBC11,an ATP binding cassette(ABC)transporter that is required for cuticular lipid secretion. The Plant Journal, 52:485-498. doi: 10.1111/tpj.2008.52.issue-3 URL
[3]	Banasiak J, Jasiński M. 2022. ATP-binding cassette transporters in nonmodel plants. New Phytol, 233:1597-1612. doi: 10.1111/nph.v233.4 URL
[4]	Chang Jun. 2023. Mining and functional analysis of ABCG genes related to benzaldehyde transport in Prunus mume[M. D. Dissertation]. Taiyuan: Shanxi Agricultural University. (in Chinese)
	常珺. 2023. 梅花苯甲醛转运相关ABCG基因的挖掘与功能分析[硕士论文]. 太原: 山西农业大学.
[5]	Chang Y L, Huang L M, Kuo X Z, Chen Y Y, Lin S T, Jeng M F, Yeh H H, Tsai W C, Chen H H. 2023. PbABCG1 and PbABCG 2 transporters are required for the emission of floral monoterpenes in Phalaenopsis bellina. Plant Journal, 114:279-292. doi: 10.1111/tpj.v114.2 URL
[6]	Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. Tbtools,an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1-10. doi: 10.1016/j.molp.2019.11.012 URL
[7]	Chen C J, Wu Y, Li J W, Wang X, Zeng Z H, Xu J, Liu Y L, Feng J T, Chen H, He Y H, Xia R. 2023. TBtools-II:a“one for all,all for one”bioinformatics platform for biological big-data mining. Molecular Plant, 16 (11):1733-1742. doi: 10.1016/j.molp.2023.09.010 URL
[8]	Choi H, Ohyama K, Kim Y Y, Jin J Y, Lee S B, Yamaoka Y, Muranaka T, Suh MC, Fujioka S, Lee Y. 2014. The role of Arabidopsis ABCG9 and ABCG 31 ATP binding cassette transporters in pollen fitness and the deposition of steryl glycosides on the pollen coat. Plant Cell, 26 (1),310-324. doi: 10.1105/tpc.113.118935 URL
[9]	Crouzet J, Roland J, Peeters E, Trombik T, Ducos E, Nader J, Boutry M. 2013. NtPDR1,a plasma membrane ABC transporter from Nicotiana tabacum,is involved in diterpene transport. Plant Molecular Biology, 82 (1-2):181-192. doi: 10.1007/s11103-013-0053-0 pmid: 23564360
[10]	Ding Donghui, Zhang Yupeng, Zhou Renling, Xi Wuyang, Geng Nizhou, Chang Wei, Zhao Qingping, Liu Jiafei, Yang Shuqiong. 2024. Identification of 7S globulin genes in Mung Bean(Vigna radiata L.) and their expression analysis during seed development. Food Science, 45 (12):109-115. (in Chinese)
	丁冬会, 张雨朋, 周人玲, 席武洋, 耿旎周, 常玮, 赵青平, 刘嘉斐, 杨树琼. 2024. 绿豆7S球蛋白基因鉴定及在籽粒发育中的表达分析. 食品科学, 45 (12):109-115.
[11]	Dudareva N, Pichersky E, Gershenzon J. 2004. Biochemistry of plant volatiles. Plant Physiology, 135 (4):1893-1902. doi: 10.1104/pp.104.049981 pmid: 15326281
[12]	Eichhorn H, Klinghammer M, Becht P, Tenhaken R. 2006. Isolation of a novel ABC-transporter gene from soybean induced by salicylic acid. Journal of Experimental Botany, 57:2193-2201. doi: 10.1093/jxb/erj179 pmid: 16720608
[13]	Fu X M, Wang H B, Tao X, Liu Y T, Chen L Q, Yang N. 2025. Integrated multiomics analysis sheds light on the mechanisms of color and fragrance biosynthesis in wintersweet flowers. International Journal of Molecular Sciences, 26 (4):1684. doi: 10.3390/ijms26041684 URL
[14]	Gerber S, Comellas-Bigler M, Goetz B A, Loche K P. 2008. Structural basis of trans-inhibition in a molybdate/tungstate ABC transporter. Science, 321 (5886):246-250. doi: 10.1126/science.1156213 pmid: 18511655
[15]	Gupta B B, Selter L L, Baranwal V K, Arora D, Mishra S K, Sirohi P, Poonia A K, Chaudhary R, Kumar R, Krattinger S G, Chauhan H. 2019. Updated inventory,evolutionary and expression analyses of G(PDR)type ABC transporter genes of rice. Plant Physiology and Biochemistry, 142:429-439. doi: 10.1016/j.plaphy.2019.08.004 URL
[16]	Han X Y, Cui M, Ma S X, Gao X M, Tang L P, Li B. 2025. Genome-wide analysis of the AarPDR gene family in Artemisia argyi and its potential role in regulating terpenoids transport. BMC Plant Biol, 25 (1):1101. doi: 10.1186/s12870-025-07144-y
[17]	Hao Ruijie, Qiu Chen, Geng Xiaoyun, Jia Haotian, Zhang Yajing, Chang Jun, Feng Xinxin. 2023. The Function of PmABCG9 transporter related to the volatilization of benzyl alcohol in Prunus mume. Scientia Agricultura Sinica, 56 (13):2574-2585. (in Chinese)
	郝瑞杰, 邱晨, 耿晓云, 贾浩田, 张雅静, 常珺, 冯新新. 2023. 梅花PmABCG9在苯甲醇挥发中的功能分析. 中国农业科学, 56 (13):2574-2585. doi: 10.3864/j.issn.0578-1752.2023.13.011
[18]	Hu Di. 2016. Floral scent composition and emission site observation of Primula forbesii[M. D. Dissertation]. Ya’an: Sichuan Agricultural University. (in Chinese)
	胡荻. 2016. 小报春(Primula forbesii)花香成分以及发香部位研究[硕士论文]. 雅安: 四川农业大学.
[19]	Hu Guiting, Yang Liyuan, Ren Guangbing, Zhao Hongbo. 2025. Floral substances and daytime release rhythms of 3 Chimonanthus species. Journal of Zhejiang A & F University, 42 (1):124-132. (in Chinese)
	胡桂婷, 杨丽媛, 任广兵, 赵宏波. 2025. 3种蜡梅属植物花香物质及白天释放节律. 浙江农林大学学报, 42 (1):124-132.
[20]	Lefevre F, Boutry. 2018. Towards identification of the substrates of ATP-binding cassette transporters. Plant Physiology, 178:18-39. doi: 10.1104/pp.18.00325 pmid: 29987003
[21]	Li Hanghang. 2020. Study on the mechanism of ABCG transporters in the adaptation of pigeon pea(Cajanus cajan)to abiotic stresses[M. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	李航航. 2020. 木豆ABCG转运蛋白抵御非生物胁迫的作用机制研究[硕士论文]. 北京: 北京林业大学.
[22]	Li Jifu, Han Jiarui, Jia Yidan, Zhang Langzhi, Wang Wenqiang, Wang Zhiyong, Chen Zhijian. 2019. Cloning and expression analysis of AcABCG1 in Axonopus compressus. Acta Agrestia Sinica, 27 (5):1147-1153. (in Chinese) doi: 10.11733/j.issn.1007-0435.2019.05.005
	李季肤, 韩佳芮, 贾怡丹, 张郎织, 王文强, 王志勇, 陈志坚. 2019. 地毯草铝响应基因AcABCG1的克隆与表达分析. 草地学报, 27 (5):1147-1153. doi: 10.11733/j.issn.1007-0435.2019.05.005
[23]	Li Y, Zhang J H, Chen H Y, Luo H M. 2021. Genome-wide analysis of ATP-binding cassette transporter provides insight to genes related to bioactive metabolite transportation in Salvia miltiorrhiza. BMC Genomics, 22 (1):315. doi: 10.1186/s12864-021-07623-0
[24]	Li Yingying. 2012. Biosynthesis and affecting factors of terpenes and phenylpropanoids volatiles in plant flowers. Biotechnology, 22 (2):86-90. (in Chinese)
	李莹莹. 2012. 萜烯类与苯丙酸类花香挥发物的生物合成与调节. 生物技术, 22 (2):86-90.
[25]	Li Yuanying. 2021. Cloning and functional analysis of MsABCG1,an ABC transporter gene from Medicago sativa[M. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	李媛英. 2021. 紫花苜蓿ABC转运蛋白基因(MsABCG1)的克隆及功能分析[硕士论文]. 杨凌: 西北农林科技大学.
[26]	Li Ziyang, Yang Kebin, Zhu Chenglei, Liu Yan, Guo Dong, Xiao Xiaoyan, Gao Zhimin. 2023. Identification and expression pattern analysis of ABCG genes in Moso bamboo. Journal of Nuclear Agricultural Sciences, 37 (5):917-926. (in Chinese) doi: 10.11869/j.issn.1000-8551.2023.05.0917
	李紫阳, 杨克彬, 朱成磊, 刘燕, 郭栋, 肖晓燕, 高志民. 2023. 毛竹ABCG基因鉴定及其表达模式研究. 核农学报, 37 (5):917-926. doi: 10.11869/j.issn.1000-8551.2023.05.0917
[27]	Lin Yuxue, Hu Yihang, Cai Yueqin, Xiong Yu, Chen Longqing, Qiao Zhenglin. 2025. Identification and expression analysis of GRF gene family in Nelumbo nucifera exogenous GA₃ and NAA treatments. Southwest China Journal of Agricultural Sciences, 38 (5):938-949. (in Chinese)
	林玉雪, 胡一航, 蔡月琴, 熊瑜, 陈龙清, 谯正林. 2025. 荷花GRF基因家族鉴定及外源GA₃和NAA处理表达分析. 西南农业学报, 38 (5):938-949.
[28]	Liu L P, Zhao L H, Chen P J, Cai H Y, Hou Z M, Jin X Y, Aslam M, Chai M N, Lai L Y, He Q, Liu Y H, Huang X Y, Chen H H, Chen Y Z, Qin Y. 2020. ATP binding cassette transporters ABCG1 and ABCG 16 affect reproductive development via auxin signalling in Arabidopsis. Plant Journal, 102 (6):1172-1186. doi: 10.1111/tpj.v102.6 URL
[29]	Liu Yanqing, Zhao Yongfang. 2017. Structure and mechanism of ABC transporter. Chinese Bulletin of Life Sciences, 29 (3):223-229. (in Chinese)
	刘艳青, 赵永芳. 2017. ABC转运蛋白结构与转运机制的研究进展. 生命科学, 29 (3):223-229.
[30]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCTmethod. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[31]	Matsuda S, Funabiki A, Furukawa K, Komori N, Koike M, Tokuji Y, Takamure I, Kato K. 2012. Genome-wide analysis and expression profiling of half-size ABC protein subgroup G in rice in response to abiotic stress and phytohormone treatments. Molecular Genetics and Genomics, 287 (10):819-835. pmid: 22996334
[32]	Moerkercke V A, Galván-Ampudia C S, Verdonk J C, Haring M A, Schuurink R C. 2012. Regulators of floral fragrance production and their targetgenes in petunia are not exclusively active in the epidermal cells of petals. Journal of Experimental Botany, 63 (8):3157-3171. doi: 10.1093/jxb/ers034 pmid: 22345641
[33]	Nguyen V N T, Buyl L S, Chung S M, Gynheung A, Ki-Hong J. 2018. OsABCG9 is an important ABC transporter of cuticular wax deposition in rice. Frontiers in Plant Science,9,960.
[34]	Panikashvilia D, Shi X, Samuel B. 2010. The Arabidopsis DSO/ABCG 11 transporter affects cutin metabolism in reproductive or gans and suberin in roots. Molecular Plant, 3:563-575. doi: 10.1093/mp/ssp103 pmid: 20035035
[35]	Pighin J A, Zheng H, Balakshin L J, Goodman I P, Western T L, Jetter R, Kunst L, Samuels A L. 2004. Plant cuticular lipid export requires an ABC transporter. Science, 306:702-704. doi: 10.1126/science.1102331 pmid: 15499022
[36]	Qian Xiaohui, Chen Longqing, Li Biao, Shi Rui. 2021. Analysis of aroma components of different genotypes of Chimonanthus praecox in Yunnan. Southwest China Journal of Agricultural Sciences, 34 (4):834-841. (in Chinese)
	钱晓慧, 陈龙清, 李彪, 施蕊. 2021. 云南地区不同基因型蜡梅花香气成分分析. 西南农业学报, 34 (4):834-841.
[37]	Qiu Chen, Chang Jun, Wang Shuaibing, Fan Lina, Jia Haotian, Hao Ruijie. 2022. Effects of different exogenous regulators on floral scent of Prunus mume. Acta Agriculturae Jiangxi, 34 (12):46-52. (in Chinese)
	邱晨, 常珺, 王帅兵, 范丽娜, 贾浩田, 郝瑞杰. 2022. 不同外源调节剂对梅花花香性状的影响. 江西农业学报, 34 (12):46-52.
[38]	Seidl A I, Richter A, Boomer K B, Yoshinaga N, Degenhardt J, Tumlinson J H. 2014. Emission of herbivore elicitor-induced sesquiterpenes is regulated by stomatal aperture in maize(Zea mays)Seedlings. Plant,Cell & Environment, 38 (1):23-34. doi: 10.1111/pce.2015.38.issue-1 URL
[39]	Tian Jingpu. 2019. Analysis of floral volatiles biosynthetic pathways and funcyional characterization of Mono-TPSs genes from Chimonanthus praecox[Ph. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	田敬璞. 2019. 蜡梅花香生物合成途径及单萜合成酶基因功能的解析[博士论文]. 武汉: 华中农业大学.
[40]	Widhalm J R, Gutensohn M, Yoo H, Adebesin F, Qian Yichun, Guo Longyun, Jaini R, Lynch J H, Mccoy R M, Shreve J T. 2015. Identification of a plastidial phenylalanine exporter that influences flux distribution through the phenylalanine biosynthetic network. Nature Communications, 6 (1):8142-8152. doi: 10.1038/ncomms9142
[41]	Xie T, Chen C J, Li C H, Liu J R, Liu C Y, He Y H. 2018. Genome-wide investigation of WRKY gene family in pineapple:evolution and expression profiles during development and stress. BMC Genomics, 19 (1):490-508. doi: 10.1186/s12864-018-4880-x
[42]	Yadav V, Molina I, Ranathunge K, Castillo I Q, Rothstein S J, Reed J W. 2014. ABCG transporters are required for suberin and pollen wall extracellular barriers in Arabidopsis. The Plant Cell, 26 (9):3569-3588. doi: 10.1105/tpc.114.129049 URL
[43]	Yan Chaofan, Sun Xuemei, Zhong Qiwen, Shao Dengkui, Deng Changrong, Wen Junqin. 2024. Identification and bioinformatics analysis of 20S proteasome gene family in tomato. Acta Horticulturae Sinica, 51 (2):266-280. (in Chinese) doi: 10.16420/j.issn.0513-353x.2023-0833 URL
	闫超凡, 孙雪梅, 钟启文, 邵登魁, 邓昌蓉, 文军琴. 2024. 番茄20S蛋白酶体基因家族鉴定及生物信息学分析. 园艺学报, 51 (2):266-280.
[44]	Yang Shuting. 2021. Identification and specific expression patterns in flower organs of ABCG genes related to floral scent from Prunus mume.[M. D. Dissertation]. Taiyuan: Shanxi Agricultural University. (in Chinese)
	杨姝婷. 2021. 梅花花香挥发的部位差异分析及ABCG基因的表达研究[硕士论文]. 太原: 山西农业大学.
[45]	Yu Li. 2013. The study of volatile components and flower pigments in Chimonanthus praecox[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	余莉. 2013. 蜡梅花挥发性组分与花色色素分析[硕士论文]. 武汉: 华中农业大学.
[46]	Zhang Yajing. 2022. Functional analysis of the PmABCG9 gene related to benzyl alcohol volatilization in Prunus mume[M. D. Dissertation]. Taiyuan: Shanxi Agricultural University. (in Chinese)
	张雅静. 2022. 梅花苯甲醇挥发相关基因PmABCG9的功能解析[硕士论文]. 太原: 山西农业大学.
[47]	Zhang Yajing, Hao Ruijie, Yang Shuting, Zhang Zhongqiang, Chang Jun, Qiu Chen. 2022. Effects of three frarance regulators on aromatic traits of Petunia hybrida flower. Acta Agriculturae Jiangxi,(3):346-355. (in Chinese)
	张雅静, 郝瑞杰, 杨姝婷, 张忠强, 常珺, 邱晨. 2022. 3种花香调节剂对矮牵牛花香性状的影响. 山西农业科学,(3):346-355.
[48]	Zhou Mingqin, Xiang Lin, Chen Longqing. 2007. Preliminary study on the aroma and color pigment components of Chimonanthus praecox. Journal of Beijing Forestry University,(1):22-25. (in Chinese)
	周明芹, 向林, 陈龙清. 2007. 蜡梅花香及花色色素成分的初步研究. 北京林业大学学报,(1):22-25.

目的基因 Gene name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
CpABCG1.2	ACGAGCTTTGGAGAAGTGGA	ACAAAGGGAAAGCGCAATGT
CpABCG1.3	GTTCCAAGATGGACGCCTGA	GGTATCCACGTCAACTCGCA
CpABCG6	ATTCAATCGGTCATGGCAGC	GCTTCCCACGTGATATGCTG
CpABCG9.1	CGCCAAAATGACAGCGGAAA	CGGCTCGCCATTGTAGGTTA
CpABCG9.2	CTGGCAAACAGAAGGCATCG	AGTGAGGAGGGTCGTTTTGC
CpABCG14	GAGATTCTGGCAATGCTGGG	CCTGTACGCCTTTTCATGGG
CpABCG20	ATCGCCGATCTTCCTACGTT	TGTAGCCCACCATCACATGT
CpABCG21	TACGGTGGTCGAAATGGCAA	GAGCCCGATCCAGCTTAGAC
CpABCG22.2	GTCGAACCATGGCCTCCTAC	GGAGGTGGCGGTGAATTGTA
CpABCG25.1	TGGATTCGTCACCCAAGACG	TCTCACATTTTCCGAGCCCC
CpABCG25.2	TGAGGAGAAGGTGGTAGGGA	ATGACCTGTCCCACCTTGAG
CpABCG26	CTTGCACTAATGGGCCCTTC	TCGGAAGTCTTAGAAGCGCA
CpABCG39.4	GGCTGTCAACTGAACAACGT	CTGGGTTGGTGAATTGTGCA
CpRPL8	ACATTGCCGACCATGAGATTG	CACTTGCCCGAGTTACCTTT

目的基因 Gene name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
CpABCG1.2	ACGAGCTTTGGAGAAGTGGA	ACAAAGGGAAAGCGCAATGT
CpABCG1.3	GTTCCAAGATGGACGCCTGA	GGTATCCACGTCAACTCGCA
CpABCG6	ATTCAATCGGTCATGGCAGC	GCTTCCCACGTGATATGCTG
CpABCG9.1	CGCCAAAATGACAGCGGAAA	CGGCTCGCCATTGTAGGTTA
CpABCG9.2	CTGGCAAACAGAAGGCATCG	AGTGAGGAGGGTCGTTTTGC
CpABCG14	GAGATTCTGGCAATGCTGGG	CCTGTACGCCTTTTCATGGG
CpABCG20	ATCGCCGATCTTCCTACGTT	TGTAGCCCACCATCACATGT
CpABCG21	TACGGTGGTCGAAATGGCAA	GAGCCCGATCCAGCTTAGAC
CpABCG22.2	GTCGAACCATGGCCTCCTAC	GGAGGTGGCGGTGAATTGTA
CpABCG25.1	TGGATTCGTCACCCAAGACG	TCTCACATTTTCCGAGCCCC
CpABCG25.2	TGAGGAGAAGGTGGTAGGGA	ATGACCTGTCCCACCTTGAG
CpABCG26	CTTGCACTAATGGGCCCTTC	TCGGAAGTCTTAGAAGCGCA
CpABCG39.4	GGCTGTCAACTGAACAACGT	CTGGGTTGGTGAATTGTGCA
CpRPL8	ACATTGCCGACCATGAGATTG	CACTTGCCCGAGTTACCTTT

基因名称 Gene name	长度/bp Length of CDS	等电点 Theoretical （pI）	分子量/kD Molecular weight	氨基酸数 Number of amino acid	亲水系数 GRAVY	不稳定系数 Instability index	信号肽 Signal peptide	亚细胞定位 Subcellular localization	类型 Type
CpABCG1.1	2 232	8.90	82.59	743	-0.051	45.95	无No	质膜Plas	WBC
CpABCG1.2	2 229	8.75	82.26	742	-0.043	46.12	无No	质膜Plas	WBC
CpABCG1.3	2 364	8.60	86.73	787	-0.010	46.63	无No	质膜Plas	WBC
CpABCG1.4	2 145	9.02	79.74	714	0.107	36.60	无No	质膜Plas	WBC
CpABCG3	2 172	8.98	80.80	723	0.197	42.75	无No	质膜Plas	WBC
CpABCG5.1	2 211	8.68	81.94	736	0.082	41.88	无No	质膜Plas	WBC
CpABCG5.2	1 959	8.41	73.56	652	0.056	45.77	无No	质膜Plas	WBC
CpABCG6	2 247	9.59	82.79	748	0.117	41.47	无No	质膜Plas	WBC
CpABCG8	1 782	9.21	65.75	593	0.410	49.37	无No	质膜Plas	WBC
CpABCG9.1	1 761	8.37	65.12	586	0.209	38.68	无No	质膜Plas	WBC
CpABCG9.2	966	7.63	34.83	321	-0.058	31.55	无No	质膜/高尔基体 Plas/golg	WBC
CpABCG11.1	2 229	8.94	82.62	742	-0.070	44.90	无No	质膜Plas	WBC
CpABCG11.2	2 094	9.04	77.71	697	0.165	34.51	无No	质膜Plas	WBC
CpABCG14	1 992	8.54	74.14	663	0.050	44.36	无No	质膜Plas	WBC
CpABCG15	2 088	9.36	77.85	695	0.041	46.45	无No	质膜Plas	WBC
CpABCG17.1	2 169	9.51	81.28	722	-0.014	40.40	无No	质膜Plas	WBC
CpABCG17.2	2 187	9.21	82.01	728	-0.085	42.97	无No	质膜Plas	WBC
CpABCG18	2 414	9.03	90.93	803	-0.112	43.72	无No	质膜Plas	WBC
CpABCG20	2 286	9.04	84.25	761	0.120	42.68	无No	质膜Plas	WBC
CpABCG21	2 034	9.01	75.41	677	-0.057	43.57	无No	质膜Plas	WBC
CpABCG22.1	2 196	9.10	81.22	731	0.069	34.65	无No	质膜Plas	WBC
CpABCG22.2	1 965	8.53	73.40	654	0.160	42.80	无No	质膜Plas	WBC
CpABCG22.3	2 235	9.15	82.50	744	0.009	34.24	无No	质膜Plas	WBC
CpABCG25.1	1 947	9.13	71.70	648	0.143	38.56	无No	质膜Plas	WBC
CpABCG25.2	1 869	6.48	68.92	622	0.189	38.45	无No	质膜Plas	WBC
CpABCG26	1 911	9.25	72.63	636	-0.120	45.59	无No	质膜Plas	WBC
CpABCG31.1	4 311	6.92	164.08	1 436	0.063	40.34	无No	质膜Plas	PDR
CpABCG31.2	4 002	9.01	149.07	1 333	0.185	35.94	无No	质膜Plas	PDR
CpABCG32	2 172	6.37	81.98	723	0.020	38.03	无No	质膜Plas	PDR
CpABCG34	4 416	6.39	166.47	1 471	0.058	38.26	无No	质膜Plas	PDR
CpABCG39.1	4 059	8.27	152.16	1 352	0.033	40.48	无No	质膜Plas	PDR
CpABCG39.2	4 167	8.31	157.99	1 388	0.026	42.55	无No	质膜Plas	PDR
CpABCG39.3	3 909	7.81	147.66	1 302	0.079	41.67	无No	质膜Plas	PDR
CpABCG39.4	4 356	8.27	163.79	1 451	0.030	40.50	无No	质膜Plas	PDR
CpABCG39.5	4 356	8.00	163.87	1 451	0.027	39.37	无No	质膜Plas	PDR
CpABCG39.6	4 314	7.49	162.94	1 437	0.062	35.65	无No	质膜Plas	PDR
CpABCG39.7	4 314	8.37	162.21	1 437	0.039	37.01	无No	质膜Plas	PDR
CpABCG39.8	4 224	8.78	158.83	1 407	0.025	35.73	无No	质膜Plas	PDR
CpABCG39.9	4 206	8.89	158.29	1 401	0.014	37.71	无No	质膜Plas	PDR
CpABCG39.10	2 079	6.32	78.08	692	-0.002	38.27	无No	质膜Plas	PDR
CpABCG39.11	4 365	6.60	163.87	1 454	0.077	38.39	无No	质膜Plas	PDR
CpABCG39.12	4 347	8.41	163.47	1 448	0.017	36.28	无No	质膜Plas	PDR
CpABCG41	3 879	8.69	146.26	1 292	0.114	40.77	无No	质膜Plas	PDR
CpABCG42	4 491	8.70	169.63	1 496	-0.031	38.12	无No	质膜Plas	PDR

基因名称 Gene name	长度/bp Length of CDS	等电点 Theoretical （pI）	分子量/kD Molecular weight	氨基酸数 Number of amino acid	亲水系数 GRAVY	不稳定系数 Instability index	信号肽 Signal peptide	亚细胞定位 Subcellular localization	类型 Type
CpABCG1.1	2 232	8.90	82.59	743	-0.051	45.95	无No	质膜Plas	WBC
CpABCG1.2	2 229	8.75	82.26	742	-0.043	46.12	无No	质膜Plas	WBC
CpABCG1.3	2 364	8.60	86.73	787	-0.010	46.63	无No	质膜Plas	WBC
CpABCG1.4	2 145	9.02	79.74	714	0.107	36.60	无No	质膜Plas	WBC
CpABCG3	2 172	8.98	80.80	723	0.197	42.75	无No	质膜Plas	WBC
CpABCG5.1	2 211	8.68	81.94	736	0.082	41.88	无No	质膜Plas	WBC
CpABCG5.2	1 959	8.41	73.56	652	0.056	45.77	无No	质膜Plas	WBC
CpABCG6	2 247	9.59	82.79	748	0.117	41.47	无No	质膜Plas	WBC
CpABCG8	1 782	9.21	65.75	593	0.410	49.37	无No	质膜Plas	WBC
CpABCG9.1	1 761	8.37	65.12	586	0.209	38.68	无No	质膜Plas	WBC
CpABCG9.2	966	7.63	34.83	321	-0.058	31.55	无No	质膜/高尔基体 Plas/golg	WBC
CpABCG11.1	2 229	8.94	82.62	742	-0.070	44.90	无No	质膜Plas	WBC
CpABCG11.2	2 094	9.04	77.71	697	0.165	34.51	无No	质膜Plas	WBC
CpABCG14	1 992	8.54	74.14	663	0.050	44.36	无No	质膜Plas	WBC
CpABCG15	2 088	9.36	77.85	695	0.041	46.45	无No	质膜Plas	WBC
CpABCG17.1	2 169	9.51	81.28	722	-0.014	40.40	无No	质膜Plas	WBC
CpABCG17.2	2 187	9.21	82.01	728	-0.085	42.97	无No	质膜Plas	WBC
CpABCG18	2 414	9.03	90.93	803	-0.112	43.72	无No	质膜Plas	WBC
CpABCG20	2 286	9.04	84.25	761	0.120	42.68	无No	质膜Plas	WBC
CpABCG21	2 034	9.01	75.41	677	-0.057	43.57	无No	质膜Plas	WBC
CpABCG22.1	2 196	9.10	81.22	731	0.069	34.65	无No	质膜Plas	WBC
CpABCG22.2	1 965	8.53	73.40	654	0.160	42.80	无No	质膜Plas	WBC
CpABCG22.3	2 235	9.15	82.50	744	0.009	34.24	无No	质膜Plas	WBC
CpABCG25.1	1 947	9.13	71.70	648	0.143	38.56	无No	质膜Plas	WBC
CpABCG25.2	1 869	6.48	68.92	622	0.189	38.45	无No	质膜Plas	WBC
CpABCG26	1 911	9.25	72.63	636	-0.120	45.59	无No	质膜Plas	WBC
CpABCG31.1	4 311	6.92	164.08	1 436	0.063	40.34	无No	质膜Plas	PDR
CpABCG31.2	4 002	9.01	149.07	1 333	0.185	35.94	无No	质膜Plas	PDR
CpABCG32	2 172	6.37	81.98	723	0.020	38.03	无No	质膜Plas	PDR
CpABCG34	4 416	6.39	166.47	1 471	0.058	38.26	无No	质膜Plas	PDR
CpABCG39.1	4 059	8.27	152.16	1 352	0.033	40.48	无No	质膜Plas	PDR
CpABCG39.2	4 167	8.31	157.99	1 388	0.026	42.55	无No	质膜Plas	PDR
CpABCG39.3	3 909	7.81	147.66	1 302	0.079	41.67	无No	质膜Plas	PDR
CpABCG39.4	4 356	8.27	163.79	1 451	0.030	40.50	无No	质膜Plas	PDR
CpABCG39.5	4 356	8.00	163.87	1 451	0.027	39.37	无No	质膜Plas	PDR
CpABCG39.6	4 314	7.49	162.94	1 437	0.062	35.65	无No	质膜Plas	PDR
CpABCG39.7	4 314	8.37	162.21	1 437	0.039	37.01	无No	质膜Plas	PDR
CpABCG39.8	4 224	8.78	158.83	1 407	0.025	35.73	无No	质膜Plas	PDR
CpABCG39.9	4 206	8.89	158.29	1 401	0.014	37.71	无No	质膜Plas	PDR
CpABCG39.10	2 079	6.32	78.08	692	-0.002	38.27	无No	质膜Plas	PDR
CpABCG39.11	4 365	6.60	163.87	1 454	0.077	38.39	无No	质膜Plas	PDR
CpABCG39.12	4 347	8.41	163.47	1 448	0.017	36.28	无No	质膜Plas	PDR
CpABCG41	3 879	8.69	146.26	1 292	0.114	40.77	无No	质膜Plas	PDR
CpABCG42	4 491	8.70	169.63	1 496	-0.031	38.12	无No	质膜Plas	PDR

基序名称 Motif	期望值 E-value	位点 Sites	宽度 Width
Motif1	3.8e-823	44	29
Motif2	2.5e-1050	43	50
Motif3	1.3e-892	44	41
Motif4	7.3e-707	44	29
Motif5	3.0e-821	40	50
Motif6	2.7e-653	16	50
Motif 7	4.0e-609	16	50
Motif 8	9.9e-583	18	50
Motif 9	2.2e-542	15	50
Motif 10	1.8e-555	18	50

Screening of ABCG Genes Related to the Transport of Volatile Compounds in Chimonanthus praecox

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 18

References 48

Related Articles 9

Recommended Articles

Metrics

Comments

类型 Type	功能 Function	顺式作用元件 cis-Acting element	数量 Number
植物生长发育 Plant growth and development	DNA 结合蛋白（ATBP-1）结合位点 Binding site of AT-rich DNA binding protein（ATBP-1）	AT-rich element	2
	MYBHv1结合位点 MYBHv1 binding site	CCAAT-box	26
	玉米醇溶蛋白代谢调节 Zein metabolism regulation	O2-site	34
	分生组织表达 Meristem expression	CAT-box，NON-box	31
	光响应元件 Light responsive element	AE-box，ATC-Motif，chs-CMA1a，MRE，G-Box，ACE，3-AF1 binding site，CAG-Motif	405
	胚乳表达 Endosperm expression	GCN4_Motif	14
	细胞周期调节 Cell cycle regulation	MSA-like	6
	栅栏叶肉细胞分化 Differentiation of the palisade mesophyll cells	HD-Zip 1	4
	昼夜节律控制 Ircadian control	circadian	14
胁迫响应 Biological and abiotic stress	低温响应 Low-temperature responsiveness	LTR	23
	防御和胁迫响应 Defense and stress responsiveness	TC-rich repeats	9
	干旱诱导 Drought-inducibility	MBS	37
	与缺氧特异性诱导有关的增强子元件，厌氧诱导所必需的调节元件 Anoxic specific inducibility，anaerobic induction	GC-Motif，ARE	66
激素响应 Hormones	脱落酸响应 Abscisic acid responsiveness	TCA-element	94
	赤霉素响应 Gibberellin-responsive element	CARE；TATC-box	49
	茉莉酸甲酯响应 The MeJA-responsiveness	CGTCA-Motif	190
	生长素响应 Auxin-responsive element	AuxRR-core，TGA-element	29
	水杨酸响应 Salicylic acid responsiveness	SARE	23

基因名称 Gene name	同源基因 Homologous gene	功能 Function	参考文献 Reference
CpABCG1.2 CpABCG1.3	PhABCG1	将挥发性苯类/苯丙素类化合物转运到花瓣表皮细胞外,促进矮牵牛花香物质的释放 Volatile benzenes/phenylpropyl compounds were transported to the outside ofpetal epidermal cells to promote the release of floral substances in petunia	Adebesin et al.,2017
	AtABCG1	参与木栓质和花粉壁细胞外屏障形成，还可影响拟南芥生殖器官中生长素的信号传导 Involved in the formation of extracellular barriers and modulation of auxin signaling in Arabidopsis reproductive organs	Liu et al.,2020
	AcABCG1	参与重金属/逆境胁迫响应 Involvement in heavy metal/stress response	李季肤等,2019
	MsABCG1	通过调节生理指标提高植物抗旱性 The drought resistance of plants can be improved by regulating physiological indexes	李媛英,2021
CpABCG14 CpABCG21 CpABCG9.1 CpABCG9.2 CpABCG25.2	PmABCG9	转运苯甲醇 Transport benzyl alcohol	郝瑞杰等,2023
	AtABCG9	促进花粉表面甾醇苷的运输从而调控花粉壁的成熟 Promote pollen surface steryl glycoside transport to regulate pollen wall maturation	Choi et al.,2014
	OsABCG9	参与水稻蜡质积累 Involved in rice wax accumulation	Nguyen et al.,2018
CpABCG26	PmABCG2	参与苯甲醛转运 Transport benzaldehyde	常珺,2023

[1]	YUE Zhiyi, LI Xin, WANG Haoning, ZHANG Qixiang, SUN Lidan. Research Progress on Floral Fragrance in Prunus mume [J]. Acta Horticulturae Sinica, 2026, 53(2): 467-480.
[2]	LIU Tinghan, ZHANG Yifan, CHEN Yunyi, ZHOU Lijun, LIU Yuchen, WU Sihui, LUO Le, YU Chao. Analysis of Flower Fragrance Components and Study on Fragrance Release Parts in Hybrid Offspring of Rosa gigantea [J]. Acta Horticulturae Sinica, 2026, 53(2): 585-597.
[3]	WANG Feilong, LIANG Qianyan, SHANG Junzhong, CHEN Longqing, XIANG Lin. Cloning，Expression and Functional Identification of CpBEAT Gene in Chimonanthus praecox [J]. Acta Horticulturae Sinica, 2025, 52(1): 160-170.
[4]	REN Fei, LU Miaomiao, LIU Jixiang, CHEN Xinli, LIU Daofeng, SUI Shunzhao, MA Jing. Expression and Adversity Resistance Analysis of a Late Embryogenesis Abundant Protein Gene CpLEA from Chimonanthus praecox [J]. Acta Horticulturae Sinica, 2023, 50(2): 359-370.
[5]	TIAN Mingkang, XU Zhixiang, LIU Xiuqun, SUI Shunzhao, LI Mingyang, LI Zhineng. Identification of the AP2 Subfamily Transcription Factors in Chimonanthus praecox and the Functional Study of CpAP2-L11 [J]. Acta Horticulturae Sinica, 2023, 50(2): 382-396.
[6]	SONG Xingrong, YUAN Puying, HE Xiangda. A New Chimonanthus praecox Cultivar‘Bianzaosu’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2059-2060.
[7]	ZHANG Qian, YANG Nan, SANG Haiyu, ZHAO Rong, SONG Xiaoxi, CHEN Longqing, XIANG Lin, ZHAO Kaige. Cloning and Functional Analysis of CpTT8 Related to Anthocyanin Synthesis in Wintersweet（Chimonanthus praecox） [J]. Acta Horticulturae Sinica, 2021, 48(10): 1945-1955.
[8]	SONG Xingrong, YUAN Puying, and HE Xiangda. A New Cultivar of Chimonanthus praecox‘Juanbei Jinpan’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 3107-3108.
[9]	YUE Yue-chong and FAN Yan-ping. The Terpene Synthases and Regulation of Terpene Metabolism in Plants [J]. ACTA HORTICULTURAE SINICA, 2011, 38(2): 379-379–388.