Research Progress on the Mechanism of Verticillium dahliae Conidia- Induced Plant Verticillium Wilt

doi:10.16420/j.issn.0513-353x.2025-0514

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (10): 3167-3184.doi: 10.16420/j.issn.0513-353x.2025-0514

Research Progress on the Mechanism of Verticillium dahliae Conidia- Induced Plant Verticillium Wilt

LIU Tao¹^,^*(), LI Hua¹, HAN Zijing¹, CAO Yonghong², and WANG Juanfei³

¹ School of Agriculture， Ningxia University， Yinchuan 750021， China
² Wulanqab City Garden Service Center， Wulanqab， Inner Mongolia 012000， China
³ Industrial Crop Institute， Shanxi Agricultural University， Taiyuan 030000， China

Received:2026-05-13 Revised:2026-08-17 Online:2026-10-25 Published:2026-10-26
Contact: LIU Tao

Abstract

Abstract:

Verticillium dahliae is a soil-borne plant pathogenic fungus with severe damage. Its conidia as the initial inoculum plays a key role in the occurrence of wilt disease. This article systematically reviews the environmental factors and regulatory genes influencing conidiation and germination of V. dahliae，and summarizes the pathogenic mechanism of V. dahliae causing wilt disease in plants from two aspects: virulence factors（reactive oxygen species，secretory proteins，hormones and secondary metabolites）during the infection process of V. dahliae and host defense mechanisms（plant hormone signals，lignin，cuticle，etc.）. Finally，future research directions are proposed，including elucidating the regulatory mechanisms of root exudate-induced conidial germination，conidial colonization in vascular bundles，and the molecular recognition mechanism between the pathogen and its host.

Key words: Verticillium wilt, Verticillium dahliae, conidia, pathogenesis, virulence factors, host defense

CLC Number:

S 432.1

LIU Tao, LI Hua, HAN Zijing, CAO Yonghong, and WANG Juanfei. Research Progress on the Mechanism of Verticillium dahliae Conidia- Induced Plant Verticillium Wilt[J]. Acta Horticulturae Sinica, 2026, 53(10): 3167-3184.

Figures/Tables 3

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分类 Classification	菌株 Strains	生防潜力 Biocontrol potential	引用文献 Reference
真菌 Fungus	Talaromyces purpureogenus	拮抗物质作用于大丽轮枝菌的线粒体和细胞膜，抑制其生长，抑制率达53% The antagonistic substance acts on the mitochondria and cell membrane of Verticillium dahliae，inhibiting its growth with an inhibition rate of 53%	Li et al.,2025
	Trichoderma koningiopsis	挥发性有机化合物抑制大丽轮枝菌菌丝体的生长，并降低烟草黄萎病的严重程度 Volatile organic compounds inhibited the growth of V. dahliae mycelium and reduced the severity of Verticillium wilt in tobacco	Kong et al.,2022
	Fusarium oxysporum	减轻大丽轮枝菌在辣椒上引发的萎蔫严重程度 It reduces the severity of wilt caused by Verticillium dahliae in pepper	Veloso et al.,2016
	Chaetomium globosum	Chaetoviridin A抑制大丽轮枝菌的生长 Chaetoviridin A inhibits the growth of V. dahliae	Zhang et al.,2021
	Cadophora sp.	粗提物抑制大丽轮枝菌的生长 The crude extract inhibits the growth of V. dahliae	Yakti et al.,2019
	Purpureocillium lilacinum	室内茄子黄萎病的发病率下降83.82%，田间病情指数降幅达40.1% In greenhouse，the incidence of eggplant Verticillium wilt was reduced by 83.82%，while the disease index decreased by 40.1% under field conditions	Lan et al.,2017
	Piriformospora indica	显著抑制大丽轮枝菌生长并降低拟南芥黄萎病 It significantly inhibits V. dahliae growth and reduces Verticillium wilt in Arabidopsis thaliana	Sun et al.,2014
细菌 Bacterium	Bacillus subtilis	抑制大丽轮枝菌生长并有效抑制番茄黄萎病发生 It inhibits the mycelial growth of V. dahliae and effectively suppress the occurrence of tomato Verticillium wilt	Pei et al.,2021
	Burkholderia gladioli	抑制棉花幼苗出现黄萎病，并通过影响油菜素内酯、赤霉素和生长素的生物合成促进棉花生长 It suppresses Verticillium wilt in cotton seedlings and promotes cotton growth by modulating the biosynthesis of brassinosteroids，gibberellins，and auxins	Wang et al.,2024
	B. altitudinis	对大丽轮枝菌生长的抑制率达73.97%，对棉花黄萎病的防效达93.59% The inhibition rate against V. dahliae growth reached 73.97%，with a control efficacy of 93.59% against cotton Verticillium wilt	Shan et al.,2024
	Pseudomonas aeruginosa	对棉花黄萎病的生防效果达47.72% The biocontrol efficacy against Verticillium wilt reached 47.72%	Niu et al.,2024
	Streptomyces spp.	抑制菌丝生长，显著降低橄榄黄萎病 It inhibits mycelial growth and significantly reduces olive Verticillium wilt	Díaz-Díaz et al.,2024
	B. amyloliquefaciens	抑制大丽轮枝菌生长，对番茄黄萎病的生防效果为89.26% It inhibits the growth of V. dahliae and demonstrates a biocontrol efficacy of 89.26% against tomato Verticillium wilt	Pei et al.,2022

分类 Classification	菌株 Strains	生防潜力 Biocontrol potential	引用文献 Reference
真菌 Fungus	Talaromyces purpureogenus	拮抗物质作用于大丽轮枝菌的线粒体和细胞膜，抑制其生长，抑制率达53% The antagonistic substance acts on the mitochondria and cell membrane of Verticillium dahliae，inhibiting its growth with an inhibition rate of 53%	Li et al.,2025
	Trichoderma koningiopsis	挥发性有机化合物抑制大丽轮枝菌菌丝体的生长，并降低烟草黄萎病的严重程度 Volatile organic compounds inhibited the growth of V. dahliae mycelium and reduced the severity of Verticillium wilt in tobacco	Kong et al.,2022
	Fusarium oxysporum	减轻大丽轮枝菌在辣椒上引发的萎蔫严重程度 It reduces the severity of wilt caused by Verticillium dahliae in pepper	Veloso et al.,2016
	Chaetomium globosum	Chaetoviridin A抑制大丽轮枝菌的生长 Chaetoviridin A inhibits the growth of V. dahliae	Zhang et al.,2021
	Cadophora sp.	粗提物抑制大丽轮枝菌的生长 The crude extract inhibits the growth of V. dahliae	Yakti et al.,2019
	Purpureocillium lilacinum	室内茄子黄萎病的发病率下降83.82%，田间病情指数降幅达40.1% In greenhouse，the incidence of eggplant Verticillium wilt was reduced by 83.82%，while the disease index decreased by 40.1% under field conditions	Lan et al.,2017
	Piriformospora indica	显著抑制大丽轮枝菌生长并降低拟南芥黄萎病 It significantly inhibits V. dahliae growth and reduces Verticillium wilt in Arabidopsis thaliana	Sun et al.,2014
细菌 Bacterium	Bacillus subtilis	抑制大丽轮枝菌生长并有效抑制番茄黄萎病发生 It inhibits the mycelial growth of V. dahliae and effectively suppress the occurrence of tomato Verticillium wilt	Pei et al.,2021
	Burkholderia gladioli	抑制棉花幼苗出现黄萎病，并通过影响油菜素内酯、赤霉素和生长素的生物合成促进棉花生长 It suppresses Verticillium wilt in cotton seedlings and promotes cotton growth by modulating the biosynthesis of brassinosteroids，gibberellins，and auxins	Wang et al.,2024
	B. altitudinis	对大丽轮枝菌生长的抑制率达73.97%，对棉花黄萎病的防效达93.59% The inhibition rate against V. dahliae growth reached 73.97%，with a control efficacy of 93.59% against cotton Verticillium wilt	Shan et al.,2024
	Pseudomonas aeruginosa	对棉花黄萎病的生防效果达47.72% The biocontrol efficacy against Verticillium wilt reached 47.72%	Niu et al.,2024
	Streptomyces spp.	抑制菌丝生长，显著降低橄榄黄萎病 It inhibits mycelial growth and significantly reduces olive Verticillium wilt	Díaz-Díaz et al.,2024
	B. amyloliquefaciens	抑制大丽轮枝菌生长，对番茄黄萎病的生防效果为89.26% It inhibits the growth of V. dahliae and demonstrates a biocontrol efficacy of 89.26% against tomato Verticillium wilt	Pei et al.,2022

分类 Classification	基因 Gene	描述 Description	突变体表型 Mutant phenotype	引用文献 Reference
非分泌蛋白 Non-secretory protein	VdSge1	转录调节因子 A transcriptional regulator	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Santhanam & Thomma,2013
	Vdpf	真菌特异转录因子 Fungal-specific transcription factor	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Luo et al.,2016
	VdFTF1	真菌特异转录因子 Fungal-specific transcription factor	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	Zhang et al.,2018a
	Vel1	含velvet结构域蛋白 Velvet domain-containing protein	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Höfer et al.,2021
	VdBre1	泛素连接酶 Ubiquitin ligase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Wang et al.,2021b
	VdAda1	Spt-Ada-Gcn5乙酰转移酶复合体亚基 Spt-Ada-Gcn5 Acetyltransferase complex subunit	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Geng et al.,2022
非分泌蛋白 Non-secretory protein	VdAsp1	肌醇多磷酸激酶 Inositol polyphosphate kinase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Tian et al.,2023a
分泌蛋白 Secretory protein	VdHSSP	分泌蛋白 Secretory protein	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	徐明等,2015
	VdPEL1	果胶裂解酶 Pectin lyase	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	杨远坤,2019
	VdSRP2	效应蛋白 Effector protein	孢子形成、萌发缺陷；致病力减弱 Defective spore formation and germination as well as attenuated virulence	李彪等,2023
	VdSOD1	超氧化物歧化酶 Superoxide dismutase	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	Tian et al.,2021
	VdM35	金属蛋白酶 Metalloproteinase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Lv et al.,2022
	VdGAL4	糖苷水解酶 Glycosidase hydrolase	孢子形成、萌发缺陷；致病力减弱 Defective spore formation and germination as well as attenuated virulence	Wen et al.,2023
	VdEPG1	糖苷水解酶 Glycosidase hydrolase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Liu et al.,2023
	VdTrx1	硫氧还蛋白 Thioredoxin	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Tian et al.,2023b
	VDAG_ 07742	假定分泌蛋白 Hypothetical secretory protein	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	Wang et al.,2023

分类 Classification	基因 Gene	描述 Description	突变体表型 Mutant phenotype	引用文献 Reference
非分泌蛋白 Non-secretory protein	VdSge1	转录调节因子 A transcriptional regulator	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Santhanam & Thomma,2013
	Vdpf	真菌特异转录因子 Fungal-specific transcription factor	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Luo et al.,2016
	VdFTF1	真菌特异转录因子 Fungal-specific transcription factor	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	Zhang et al.,2018a
	Vel1	含velvet结构域蛋白 Velvet domain-containing protein	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Höfer et al.,2021
	VdBre1	泛素连接酶 Ubiquitin ligase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Wang et al.,2021b
	VdAda1	Spt-Ada-Gcn5乙酰转移酶复合体亚基 Spt-Ada-Gcn5 Acetyltransferase complex subunit	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Geng et al.,2022
非分泌蛋白 Non-secretory protein	VdAsp1	肌醇多磷酸激酶 Inositol polyphosphate kinase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Tian et al.,2023a
分泌蛋白 Secretory protein	VdHSSP	分泌蛋白 Secretory protein	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	徐明等,2015
	VdPEL1	果胶裂解酶 Pectin lyase	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	杨远坤,2019
	VdSRP2	效应蛋白 Effector protein	孢子形成、萌发缺陷；致病力减弱 Defective spore formation and germination as well as attenuated virulence	李彪等,2023
	VdSOD1	超氧化物歧化酶 Superoxide dismutase	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	Tian et al.,2021
	VdM35	金属蛋白酶 Metalloproteinase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Lv et al.,2022
	VdGAL4	糖苷水解酶 Glycosidase hydrolase	孢子形成、萌发缺陷；致病力减弱 Defective spore formation and germination as well as attenuated virulence	Wen et al.,2023
	VdEPG1	糖苷水解酶 Glycosidase hydrolase	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Liu et al.,2023
	VdTrx1	硫氧还蛋白 Thioredoxin	孢子形成缺陷；致病力减弱 Defective spore formation and attenuated virulence	Tian et al.,2023b
	VDAG_ 07742	假定分泌蛋白 Hypothetical secretory protein	孢子形成正常；致病力减弱 Normal spore formation and attenuated virulence	Wang et al.,2023

Research Progress on the Mechanism of Verticillium dahliae Conidia- Induced Plant Verticillium Wilt

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