Development of Blight-Resistant and High-Pungency Processing-Type Pepper Cytoplasmic Male Sterility（CMS）Restorer Lines Using Doubled Haploid Technology

doi:10.16420/j.issn.0513-353x.2025-0343

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (4): 1189-1201.doi: 10.16420/j.issn.0513-353x.2025-0343

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Development of Blight-Resistant and High-Pungency Processing-Type Pepper Cytoplasmic Male Sterility（CMS）Restorer Lines Using Doubled Haploid Technology

LI Yifei, DUAN Minjie, HUANG Qizhong, WANG Chunping, HUANG Renzhong^*(), ZHANG Shicai^*()

Chongqing Academy of Agricultural Sciences，Chongqing 401329，China

Received:2025-12-15 Revised:2026-02-12 Online:2026-04-25 Published:2026-04-20
Contact: HUANG Renzhong, ZHANG Shicai

Abstract

Abstract:

To accelerate the development of cytoplasmic male sterility（CMS）restorer lines for processing-type peppers with disease resistance and high pungency，eight interspecific triple cross hybrids aggregated with disease resistance，strong pungency，and restorer genes-were used as experimental materials for anther culture to induce doubled haploid（DH）. The goal was to obtain DH lines that combine disease resistance，pungency，and CMS fertility restoration. The results showed that embryoids were successfully induced from all eight hybrids，with an artificial chromosome doubling rate of 89.8% in haploid seedlings. Following comprehensive evaluation using molecular marker-assisted selection，agronomic trait assessment，quality analysis，disease resistance screening，and CMS fertility restoration testing，three CMS restorer lines were selected from 12 DH lines. These lines exhibited low initial flowering nodes，strong disease resistance，and high pungency，making them ideal parental materials for breeding new processing-type pepper cultivars with desirable traits.

Key words: pepper, anther culture, molecular marker-assisted selection, resistance to Phytophthora blight, high pungency, cytoplasmic male sterility, restorer line

LI Yifei, DUAN Minjie, HUANG Qizhong, WANG Chunping, HUANG Renzhong, ZHANG Shicai. Development of Blight-Resistant and High-Pungency Processing-Type Pepper Cytoplasmic Male Sterility（CMS）Restorer Lines Using Doubled Haploid Technology[J]. Acta Horticulturae Sinica, 2026, 53(4): 1189-1201.

Figures/Tables 9

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DH系DH lines	分子标记Molecular marker	PCR检测阳性株PCR detection	所占比例/% Percentage
抗疫病 Resistance to Phytophthora blight	FQ01/RQ01	20	45.5
恢复基因 Fertility restorer gene	CRF-SCAR	34	77.3
抗疫病兼恢复基因 Disease-resistant and fertility restorer gene	FQ01/RQ01、CRF-SCAR	12	27.3

DH系DH lines	分子标记Molecular marker	PCR检测阳性株PCR detection	所占比例/% Percentage
抗疫病 Resistance to Phytophthora blight	FQ01/RQ01	20	45.5
恢复基因 Fertility restorer gene	CRF-SCAR	34	77.3
抗疫病兼恢复基因 Disease-resistant and fertility restorer gene	FQ01/RQ01、CRF-SCAR	12	27.3

供体 Anther culture donor	DH系 DH lines	株高/cm Height	冠幅/cm Scope	始花节位 First flower node	果形指数 Fruit shape index	单株挂果数 Fruit number per plant
（H2019410-7 × Bahama Hot） F₁ × PI201234	H202245-9	140.07 ± 0.31 a	95.47 ± 0.83 b	13.10 ± 0.26 a	3.51 ± 0.02 e	105.00 ± 0.40 b
	H202245-11	89.32 ± 1.13 d	99.10 ± 0.31 a	10.27 ± 0.12 cd	4.21 ± 0.01 c	99.40 ± 1.56 c
	H202245-13	86.30 ± 0.40 e	91.45 ± 0.23 c	11.80 ± 0.00 b	3.11 ± 0.01 f	207.53 ± 0.76 a
	H202245-14	85.06 ± 0.14 e	87.71 ± 1.06 d	10.07 ± 0.12 d	5.88 ± 0.00 b	53.00 ± 0.53 d
	H202245-15	130.05 ± 0.10 b	84.07 ± 0.43 e	10.93 ± 0.12 c	4.10 ± 0.02 d	39.00 ± 0.20 f
	H202245-16	115.01 ± 0.34 c	86.53 ± 0.17 d	10.76 ± 0.60 cd	6.81 ± 0.02 a	45.00 ± 0.87 e
（H2019410-7 × Naga Morich） F₁ × CM334	H202247-6	121.04 ± 0.56 a	91.49 ± 0.28 a	10.93 ± 0.31 b	6.41 ± 0.02 a	37.00 ± 0.53 b
	H202247-9	84.71 ± 0.81 c	87.32 ± 0.63 b	12.00 ± 0.20 a	4.69 ± 0.02 c	137.93 ± 0.23 a
	H202247-11	92.99 ± 0.81 b	82.01 ± 0.65 c	12.00 ± 0.20 a	6.11 ± 0.03 b	38.00 ± 0.80 b
（H2018235-3 × Bahama Hot） F₁ × PI201234	H202293-1	87.48 ± 0.82 b	71.29 ± 0.46 a	12.07 ± 0.23 a	5.80 ± 0.02 a	44.80 ± 1.11 b
	H202293-2	91.95 ± 0.53 a	68.02 ± 0.54 b	7.00 ± 0.20 c	4.80 ± 0.02 b	37.93 ± 1.10 c
	H202293-8	79.29 ± 0.25 c	69.84 ± 1.19 ab	10.00 ± 0.20 b	4.80 ± 0.02 b	92.67 ± 0.61 a

供体 Anther culture donor	DH系 DH lines	株高/cm Height	冠幅/cm Scope	始花节位 First flower node	果形指数 Fruit shape index	单株挂果数 Fruit number per plant
（H2019410-7 × Bahama Hot） F₁ × PI201234	H202245-9	140.07 ± 0.31 a	95.47 ± 0.83 b	13.10 ± 0.26 a	3.51 ± 0.02 e	105.00 ± 0.40 b
	H202245-11	89.32 ± 1.13 d	99.10 ± 0.31 a	10.27 ± 0.12 cd	4.21 ± 0.01 c	99.40 ± 1.56 c
	H202245-13	86.30 ± 0.40 e	91.45 ± 0.23 c	11.80 ± 0.00 b	3.11 ± 0.01 f	207.53 ± 0.76 a
	H202245-14	85.06 ± 0.14 e	87.71 ± 1.06 d	10.07 ± 0.12 d	5.88 ± 0.00 b	53.00 ± 0.53 d
	H202245-15	130.05 ± 0.10 b	84.07 ± 0.43 e	10.93 ± 0.12 c	4.10 ± 0.02 d	39.00 ± 0.20 f
	H202245-16	115.01 ± 0.34 c	86.53 ± 0.17 d	10.76 ± 0.60 cd	6.81 ± 0.02 a	45.00 ± 0.87 e
（H2019410-7 × Naga Morich） F₁ × CM334	H202247-6	121.04 ± 0.56 a	91.49 ± 0.28 a	10.93 ± 0.31 b	6.41 ± 0.02 a	37.00 ± 0.53 b
	H202247-9	84.71 ± 0.81 c	87.32 ± 0.63 b	12.00 ± 0.20 a	4.69 ± 0.02 c	137.93 ± 0.23 a
	H202247-11	92.99 ± 0.81 b	82.01 ± 0.65 c	12.00 ± 0.20 a	6.11 ± 0.03 b	38.00 ± 0.80 b
（H2018235-3 × Bahama Hot） F₁ × PI201234	H202293-1	87.48 ± 0.82 b	71.29 ± 0.46 a	12.07 ± 0.23 a	5.80 ± 0.02 a	44.80 ± 1.11 b
	H202293-2	91.95 ± 0.53 a	68.02 ± 0.54 b	7.00 ± 0.20 c	4.80 ± 0.02 b	37.93 ± 1.10 c
	H202293-8	79.29 ± 0.25 c	69.84 ± 1.19 ab	10.00 ± 0.20 b	4.80 ± 0.02 b	92.67 ± 0.61 a

DH系DH lines	病情指数Disease index	抗性等级Resistance level	恢复株率Plants with restoring ability/%
H202245-9	28.9	抗病 Disease resistance	100
H202245-11	19.2	抗病 Disease resistance	100
H202245-13	25.6	抗病 Disease resistance	100
H202245-14	28.1	抗病 Disease resistance	100
H202245-15	27.9	抗病 Disease resistance	100
H202245-16	25.3	抗病 Disease resistance	100
H202247-6	23.7	抗病 Disease resistance	100
H202247-9	27.5	抗病 Disease resistance	100
H202247-11	29.2	抗病 Disease resistance	100
H202293-1	29.8	抗病 Disease resistance	100
H202293-2	24.2	抗病 Disease resistance	100
H202293-8	26.4	抗病 Disease resistance	100
CK1（Early calwonder）	66.7	感病 Susceptible	/
CK2（PI201234）	7.8	高感 High susceptible	/

Development of Blight-Resistant and High-Pungency Processing-Type Pepper Cytoplasmic Male Sterility（CMS）Restorer Lines Using Doubled Haploid Technology

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编号 Plant lines	斯克维尔指数（SHU） Skewer Index	干物质含量/（g · kg^-1） Dry matter	维生素C/（g · kg^-1） Vitamin C	蛋白质/（g · kg^-1） Protein	脂肪/（g · kg^-1） Fat
H202245-11	47 972	287.00	1.06	49.20	32.00
H202247-9	52 875	226.00	0.97	25.20	28.00
H202293-8	53 523	330.00	0.61	37.20	20.00

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