Identification of Talaromyces Against Broad Bean Root Rot and Its Disease-Resistant and Growth-Promoting Effects

doi:10.16420/j.issn.0513-353x.2024-0986

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (1): 244-256.doi: 10.16420/j.issn.0513-353x.2024-0986

• Plant Protection • Previous Articles Next Articles

Identification of Talaromyces Against Broad Bean Root Rot and Its Disease-Resistant and Growth-Promoting Effects

LIU Chenwei, WANG Fan, BIAN Xiaochun, XU Renchao, LU Hongchen, WU Chunfang^*()

Key Laboratory of Crop Vernalization Technology of Nantong City， Jiangsu Yanjiang Institute of Agricultural Sciences，Nantong， Jiangsu 226012， China

Received:2025-09-08 Revised:2025-10-23 Online:2026-01-25 Published:2026-01-26
Contact: WU Chunfang

Abstract

Abstract:

Root rot is one of the primary diseases affecting broad bean，severely limiting the development of the broad bean industry. To acquire effective biocontrol fungal resources for broad bean root rot，root rot pathogens Fusarium fujikuroi and Fusarium oxysporum were selected as indicator fungi，screening fungi with strong antifungal activity from soil samples in Rugao City，Jiangsu Province. The morphological and molecular biological technology were used to identified the screened fungi. The antifungal mechanisms was preliminarily investigated，and the greenhouse pot test was performed to analyze its disease resistance and growth promoting effects. The results indicated that the isolated strains VRB1 and VRB36 exhibited significant antifungal activities against the two Fusarium，with inhibition rates exceeding 81.00%. Strain VRB1 was identified as Talaromyces purpurogenus，while VRB36 was identified as Talaromyces pinophilus. Both biocontrol fungi VRB1 and VRB36 have the ability to secrete enzymes such as protease and amylase. They have the ability to dissolve organic phosphorus，fix nitrogen and produce iron carrier. StrainVRB1 also has the ability to produce HCN. The sterile filtrate of the two Talaromyces significantly inhibited mycelial growth and spore germination of Fusarium，and affected the permeability of cell membrane. Greenhouse pot experiment showed that two Talaromyces could control the occurrence of broad bean root rot，with control efficiency of 71.31% and 56.92%，respectively. Furthermore，they significantly promoted the plant height，root length and fresh and dry weight of broad bean seedlings. These two Talaromyces have the potential to be developed as biocontrol agent against broad bean root rot.

Key words: broad bean, root rot, Talaromyces, biocontrol characteristics, control efficacy, growth promothing effect

LIU Chenwei, WANG Fan, BIAN Xiaochun, XU Renchao, LU Hongchen, WU Chunfang. Identification of Talaromyces Against Broad Bean Root Rot and Its Disease-Resistant and Growth-Promoting Effects[J]. Acta Horticulturae Sinica, 2026, 53(1): 244-256.

Figures/Tables 11

References 31

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无菌滤液浓度/% Concentration of fungus-free filtrate	接种10 d Incubation 10 d				接种24 d Incubation 24 d
无菌滤液浓度/% Concentration of fungus-free filtrate	VRB1-VFR1	VRB36-VFR1	VRB1-VFR2	VRB36-VFR2	VRB1-VFR1	VRB36-VFR1	VRB1-VFR2	VRB36-VFR2
0（对照Control）	53.77 ± 0.99 a		60.62 ± 0.90 a		84.97 ± 0.52 a		93.84 ± 0.96 a
10	37.96 ± 0.84 b	46.77 ± 0.90 b	45.39 ± 1.01 b	45.91 ± 0.43 b	66.46 ± 0.34 b	76.99 ± 0.56 b	83.23 ± 0.43 b	84.84 ± 1.00 b
30	9.33 ± 0.87 c	18.84 ± 1.01 c	18.38 ± 0.34 c	20.60 ± 0.21 c	24.01 ± 0.59 c	38.85 ± 0.48 c	39.18 ± 0.42 c	41.50 ± 0.52 c
50	3.80 ± 0.63 d	11.72 ± 0.59 d	10.91 ± 0.20 d	11.60 ± 0.17 d	5.87 ± 0.14 d	16.43 ± 0.46 d	15.74 ± 0.52 d	17.48 ± 0.34 d

无菌滤液浓度/% Concentration of fungus-free filtrate	接种10 d Incubation 10 d				接种24 d Incubation 24 d
无菌滤液浓度/% Concentration of fungus-free filtrate	VRB1-VFR1	VRB36-VFR1	VRB1-VFR2	VRB36-VFR2	VRB1-VFR1	VRB36-VFR1	VRB1-VFR2	VRB36-VFR2
0（对照Control）	53.77 ± 0.99 a		60.62 ± 0.90 a		84.97 ± 0.52 a		93.84 ± 0.96 a
10	37.96 ± 0.84 b	46.77 ± 0.90 b	45.39 ± 1.01 b	45.91 ± 0.43 b	66.46 ± 0.34 b	76.99 ± 0.56 b	83.23 ± 0.43 b	84.84 ± 1.00 b
30	9.33 ± 0.87 c	18.84 ± 1.01 c	18.38 ± 0.34 c	20.60 ± 0.21 c	24.01 ± 0.59 c	38.85 ± 0.48 c	39.18 ± 0.42 c	41.50 ± 0.52 c
50	3.80 ± 0.63 d	11.72 ± 0.59 d	10.91 ± 0.20 d	11.60 ± 0.17 d	5.87 ± 0.14 d	16.43 ± 0.46 d	15.74 ± 0.52 d	17.48 ± 0.34 d

处理Treatment		病情指数 Disease index	防治效果/% Control efficiency
清水（对照）Water（Control）		80.44 ± 3.36 a	—
预防 Pervention	VRB1	23.11 ± 2.04 c	71.31 ± 1.32 a
	VRB36	36.44 ± 2.04 b	54.66 ± 2.77 d
治疗Treament	VRB1	32.00 ± 1.33 b	60.16 ± 2.83 b
	VRB36	34.67 ± 2.31 b	56.92 ± 1.82 c

处理Treatment		病情指数 Disease index	防治效果/% Control efficiency
清水（对照）Water（Control）		80.44 ± 3.36 a	—
预防 Pervention	VRB1	23.11 ± 2.04 c	71.31 ± 1.32 a
	VRB36	36.44 ± 2.04 b	54.66 ± 2.77 d
治疗Treament	VRB1	32.00 ± 1.33 b	60.16 ± 2.83 b
	VRB36	34.67 ± 2.31 b	56.92 ± 1.82 c

品种 Cultivar	处理 Treatment	地上部Shoot			地下部Root
品种 Cultivar	处理 Treatment	株高/cm Height	鲜质量/g Fresh weight	干质量/g Dry weight	根长/cm Length	鲜质量/g Fresh weight	干质量/g Dry weight
CD-001	对照Control	16.19 ± 0.38 c	3.21 ± 0.08 c	0.51 ± 0.03 b	10.27 ± 0.46 c	1.87 ± 0.02 c	0.19 ± 0 b
	VRB1	18.08 ± 0.43 b	3.35 ± 0.57 b	0.53 ± 0.10 ab	12.51 ± 0.57 b	3.31 ± 0.02 b	0.33 ± 0.01 a
	VRB36	18.11 ± 0.29 a	3.46 ± 0.09 a	0.54 ± 0.04 a	13.02 ± 0.37 a	3.42 ± 0.02 a	0.34 ± 0.01 a
CD-003	对照Control	13.77 ± 0.49 c	1.76 ± 0.03 b	0.20 ± 0.01 b	9.43 ± 0.51 c	1.14 ± 0.03 b	0.11 ± 0 b
	VRB1	13.94 ± 0.35 b	1.79 ± 0.04 b	0.20 ± 0.01 b	10.82 ± 0.29 b	1.15 ± 0.03 b	0.11 ± 0 b
	VRB36	16.87 ± 0.46 a	2.48 ± 0.05 a	0.28 ± 0.01 a	15.46 ± 0.35 a	1.32 ± 0.02 a	0.13 ± 0 a
CD-005	对照Control	16.66 ± 0.37 c	3.00 ± 0.08 c	0.52 ± 0.04 b	8.58 ± 0.55 c	1.65 ± 0.02 c	0.13 ± 0 c
	VRB1	18.51 ± 0.33 b	3.66 ± 0.09 b	0.65 ± 0.04 a	11.05 ± 0.50 b	2.25 ± 0.01 b	0.18 ± 0 b
	VRB36	19.49 ± 0.34 a	4.24 ± 0.06 a	0.67 ± 0.05 a	11.87 ± 0.36 a	2.51 ± 0.02 a	0.20 ± 0 a
CD-008	对照Control	13.97 ± 0.52 c	3.89 ± 0.02 c	0.39 ± 0.02 b	13.71 ± 0.56 c	2.85 ± 0.03 b	0.23 ± 0 b
	VRB1	17.90 ± 0.56 b	4.43±0.05 b	0.44 ± 0.01 a	16.68 ± 0.48 b	3.41 ± 0.02 a	0.28 ± 0.01 a
	VRB36	17.93 ± 0.56 a	4.59 ± 0.02 a	0.45 ± 0.01 a	17.43 ± 0.39 a	3.41 ± 0.02 a	0.28 ± 0 a
CD-011	对照Control	11.85 ± 0.44 c	1.46 ± 0.04 c	0.20 ± 0.01 c	8.54 ± 0.31 c	1.29 ± 0.01 c	0.12 ± 0 c
	VRB1	15.41 ± 0.55 b	2.18 ± 0.02 b	0.30 ± 0.02 b	14.10 ± 0.72 b	1.64 ± 0.02 b	0.15 ± 0 b
	VRB36	18.54 ± 0.43 a	2.69 ± 0.10 a	0.37 ± 0.02 a	18.28 ± 0.35 a	1.76 ± 0.02 a	0.16 ± 0 a
CD-017	对照Control	9.42 ± 0.79 c	1.01 ± 0.05 c	0.13 ± 0.01 c	10.97 ± 0.47 c	1.33 ± 0.01 c	0.14 ± 0 c
	VRB1	13.81 ± 0.47 b	1.44 ± 0.05 b	0.18 ± 0.01 b	13.25 ± 0.34 b	1.61 ± 0.01 b	0.16 ± 0 b
	VRB36	14.28 ± 0.49 a	1.66 ± 0.05 a	0.21 ± 0.01 a	14.51 ± 0.40 a	1.67 ± 0.01 a	0.17 ± 0 a

Identification of Talaromyces Against Broad Bean Root Rot and Its Disease-Resistant and Growth-Promoting Effects

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