Identification of Phytopythium helicoides Causing Root Rot of Impatiens hawkeri and Toxicity Test of Different Fungicides in Laboratory

doi:10.16420/j.issn.0513-353x.2022-0128

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (5): 1130-1140.doi: 10.16420/j.issn.0513-353x.2022-0128

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Identification of Phytopythium helicoides Causing Root Rot of Impatiens hawkeri and Toxicity Test of Different Fungicides in Laboratory

ZHANG Xiaoyong, LI Shujiang, LONG Qiaofang, YANG Youlian()

School of Biological Science and Technology，Liupanshui Normal University，Liupanshui，Guizhou 553004，China

Received:2022-12-15 Revised:2023-03-07 Online:2023-05-25 Published:2023-05-31
Contact: YANG Youlian E-mail:yangyoulianl@163.com

Abstract

Abstract:

In order to identify the pathogen of Impatiens hawkeri root rot，two pathogenic strains were isolated from diseased roots by tissue separation method. Based on morphological characteristics and ITS and COX II sequences analysis，the pathogen strains were finally identified as Phytopythium helicoides. Inoculating the pathogen on the healthy roots，stems and leaves reproduced the heavy symptoms observed according to the Koch’s rule. The determination of host range showed that P. helicoides could infect not only I. hawkeri，but also I. balsamina，Begonia cucullata，B. elatior，Pelargonium hortorum，Rosa hybrida，R. rugosa，Fuchsia hybrida，Crassula arborescens and Kalanchoe blossfeldiana. In culture tests，the optimum for mycelial growth were at 27-32 ℃ at pH 6-7. Additionally，the toxic effect of ten fungicides against pathogen were evaluated in vitro. Among them 10% oxathiapiprolin OD，98% hymexazol SP，40% dimethomorph WG and 80% mancozeb WP performed good inhibition effects.

Key words: Impatiens hawkeri, root rot, Phytopythium helicoides, host plant range, cultural characteristics, toxicity test in laboratory

CLC Number:

S681.1

ZHANG Xiaoyong, LI Shujiang, LONG Qiaofang, YANG Youlian. Identification of Phytopythium helicoides Causing Root Rot of Impatiens hawkeri and Toxicity Test of Different Fungicides in Laboratory[J]. Acta Horticulturae Sinica, 2023, 50(5): 1130-1140.

Figures/Tables 7

References 33

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科名 Taxonomic status	寄主种类 Species	病斑长度/mm Length of lesion	致病能力 Severity
凤仙花科Balsaminaceae	凤仙花Impatiens balsamina	26.32 ± 2.33 a	++++
秋海棠科Begoniaceae	四季海棠Begonia semperflorens	24.10 ± 1.04 ab	++++
	丽格海棠Begonia elatior	22.77 ± 1.37 b	++++
柳叶菜科Onagraceae	倒挂金钟Fuchsia hybrida	15.37 ± 1.15 c	+++
绣球花科Hydrangeaceae	绣球Hydrangea macrophylla	0 e	-
菊科Asteraceae	乒乓菊Chrysanthemum morifolium var. pompon	0 e	-
	金光菊Rudbeckia laciniata	0 e	-
石竹科Caryophyllaceae	香石竹Dianthus caryophyllus var. carnation	0 e	-
牻牛儿苗科Geraniaceae	天竺葵Pelargonium hortorum	28.10 ± 1.85 a	++++
景天科Crassulaceae	玉树Crassula arborescens	10.30 ± 0.85 d	++
	长寿花Kalanchoe blossfeldiana	11.23 ± 1.36 d	++
蔷薇科Rosaceae	微型月季Rosa hybrida	24.57 ± 0.97 ab	++++
	玫瑰Rosa rugosa	26.45 ± 1.64 a	++++
天南星科Araceae	白掌Spathiphyllum patinii	0 e	-
	红掌Anthurium andraeanum	0 e	-
棕榈科Arecaceae	袖珍椰子Collinia elegans	0 e	-

科名 Taxonomic status	寄主种类 Species	病斑长度/mm Length of lesion	致病能力 Severity
凤仙花科Balsaminaceae	凤仙花Impatiens balsamina	26.32 ± 2.33 a	++++
秋海棠科Begoniaceae	四季海棠Begonia semperflorens	24.10 ± 1.04 ab	++++
	丽格海棠Begonia elatior	22.77 ± 1.37 b	++++
柳叶菜科Onagraceae	倒挂金钟Fuchsia hybrida	15.37 ± 1.15 c	+++
绣球花科Hydrangeaceae	绣球Hydrangea macrophylla	0 e	-
菊科Asteraceae	乒乓菊Chrysanthemum morifolium var. pompon	0 e	-
	金光菊Rudbeckia laciniata	0 e	-
石竹科Caryophyllaceae	香石竹Dianthus caryophyllus var. carnation	0 e	-
牻牛儿苗科Geraniaceae	天竺葵Pelargonium hortorum	28.10 ± 1.85 a	++++
景天科Crassulaceae	玉树Crassula arborescens	10.30 ± 0.85 d	++
	长寿花Kalanchoe blossfeldiana	11.23 ± 1.36 d	++
蔷薇科Rosaceae	微型月季Rosa hybrida	24.57 ± 0.97 ab	++++
	玫瑰Rosa rugosa	26.45 ± 1.64 a	++++
天南星科Araceae	白掌Spathiphyllum patinii	0 e	-
	红掌Anthurium andraeanum	0 e	-
棕榈科Arecaceae	袖珍椰子Collinia elegans	0 e	-

杀菌剂 Fungicide	毒力回归方程 Regression equation	相关系数 Correlation coefficient	抑制中浓度/（μg · mL^-1） EC₅₀
80% 多菌灵Carbendazim WP	Y = 2.9826 X + 2.2912	0.9926	8.10
75% 百菌清Chlorothalonil WP	Y = 3.1606 X + 3.9127	0.9464	2.21
25% 三唑酮Triadimefon WP	Y = 7.6882 X + 4.9517	0.9825	1.01
80% 代森锰锌Mancozeb WP	Y = 2.8019 X + 6.9145	0.9798	0.21
98% 噁霉灵Hymexazol SP	Y = 1.2229 X + 5.9622	0.9903	0.16
430 g · L^-1 戊唑醇Tebuconazole SC	Y = 5.5234 X + 1.3452	0.9977	4.59
40% 腈菌唑Myclobutanil WP	Y = 1.9854 X + 3.3355	0.9939	6.89
40% 烯酰吗啉Dimethomorph WG	Y = 2.5234 X + 6.8210	0.9977	0.19
70% 甲基硫菌灵Thi-methyl WP	Y = 0.9542 X + 4.1203	0.9987	8.35
10% 氟噻唑吡乙酮Oxathiapiprolin OD	Y = 2.3220 X + 8.5546	0.9982	0.03

杀菌剂 Fungicide	毒力回归方程 Regression equation	相关系数 Correlation coefficient	抑制中浓度/（μg · mL^-1） EC₅₀
80% 多菌灵Carbendazim WP	Y = 2.9826 X + 2.2912	0.9926	8.10
75% 百菌清Chlorothalonil WP	Y = 3.1606 X + 3.9127	0.9464	2.21
25% 三唑酮Triadimefon WP	Y = 7.6882 X + 4.9517	0.9825	1.01
80% 代森锰锌Mancozeb WP	Y = 2.8019 X + 6.9145	0.9798	0.21
98% 噁霉灵Hymexazol SP	Y = 1.2229 X + 5.9622	0.9903	0.16
430 g · L^-1 戊唑醇Tebuconazole SC	Y = 5.5234 X + 1.3452	0.9977	4.59
40% 腈菌唑Myclobutanil WP	Y = 1.9854 X + 3.3355	0.9939	6.89
40% 烯酰吗啉Dimethomorph WG	Y = 2.5234 X + 6.8210	0.9977	0.19
70% 甲基硫菌灵Thi-methyl WP	Y = 0.9542 X + 4.1203	0.9987	8.35
10% 氟噻唑吡乙酮Oxathiapiprolin OD	Y = 2.3220 X + 8.5546	0.9982	0.03

Identification of Phytopythium helicoides Causing Root Rot of Impatiens hawkeri and Toxicity Test of Different Fungicides in Laboratory

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