Development of HRM Molecular Markers for Important Traits in Tomato and Assessment of Analytical Effects Across Various Reaction Systems

doi:10.16420/j.issn.0513-353x.2024-0881

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (12): 3202-3218.doi: 10.16420/j.issn.0513-353x.2024-0881

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Development of HRM Molecular Markers for Important Traits in Tomato and Assessment of Analytical Effects Across Various Reaction Systems

LIU Chang¹, WEI Kai¹^,², ZHANG Li¹^,², LU Feifei¹, YU Qingtong¹^,³, WANG Mengya¹^,⁴, WANG Xiaoxuan¹, DU Yongchen¹, GUO Yanmei¹, LI Junming¹, LIU Lei¹, LI Xin¹, ZHU Can¹, HUANG Zejun¹^,^*()

¹ State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
² College of Horticulture，China Agricultural University，Beijing 100193，China
³ Institute of Plant Science and Technology，Beijing Agricultural University，Beijing102206，China
⁴ College of Horticulture Science，Zhejiang A & F University，Hangzhou 311300，China

Received:2025-08-06 Revised:2025-10-17 Online:2025-12-25 Published:2025-12-20
Contact: HUANG Zejun

Abstract

Abstract:

High resolution melting（HRM）genotyping technology can identify the genotypes of different samples by forming different melting curves，be characterized by its simplicity，rapid processing，high throughput，and superior resolution. This study aims to develop HRM molecular markers for identifying superior alleles associated with important traits in tomato and to assess the impact of various factors on HRM analysis outcomes. A total of 23 HRM molecular markers were successfully developed based on the sequence characteristics of 23 superior alleles of specific genes or their adjacent regions. These genes include those associated with disease resistance，such as Bwr-12，Frl，I-7，Pto，Rx4，Sm，Ty-1；genes conferring abiotic stress tolerance，including SlACR11A，SlBBX31，SlHAK20，SlSOS2；genes related to fruit traits，such as Aft，LC，GF，OVATE，PT，R，SlALMT9，STP1，Y；and genes associated with plant type，including SD1，SP，SP5G. Furthermore，an assessment of the impact of varying reaction volumes，template DNA concentrations，and DNA extraction methods on HRM analysis revealed that reaction volumes of 5，7.5，10，12.5 and 15 µL demonstrated comparable analytical performance to the recommended reaction volume of 20 µL specified in the kit. Notably，within the 5 µL reaction volume，DNA concentrations ranging from 12.5 ng · µL^-1 to 1 600 ng · µL^-1exhibited robust analytical performance. Additionally，DNA extracted using the NaOH method was found to be suitable for HRM analysis. The findings of this study will facilitate the identification of superior alleles and marker-assisted breeding in tomato.

Key words: tomato, important trait, superior allele, HRM, molecular marker

LIU Chang, WEI Kai, ZHANG Li, LU Feifei, YU Qingtong, WANG Mengya, WANG Xiaoxuan, DU Yongchen, GUO Yanmei, LI Junming, LIU Lei, LI Xin, ZHU Can, HUANG Zejun. Development of HRM Molecular Markers for Important Traits in Tomato and Assessment of Analytical Effects Across Various Reaction Systems[J]. Acta Horticulturae Sinica, 2025, 52(12): 3202-3218.

Figures/Tables 12

References 41

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品系 Line	基因型 Genotype	基因型数据来源 Source of genotypic information
15N60	SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SlHAK20^Hap1/SlHAK20^Hap1、Sm/Sm、SP5G^TS30M/SP5G^TS30M、Ty-1/Ty-1	本课题组重测序 Our resequencing results
231-13-2	Sm/Sm	公司标记检测 Company marker detection result
231-30-1	-	公司标记检测 Company marker detection result
231-35-2	Frl/Frl	公司标记检测 Company marker detection result
23N1338-1	Aft/Aft、ovate/ovate	本课题组标记检测 Our marker detection results
23N1503	r^PI114490/r^PI114490	本课题组标记检测 Our marker detection results
24N97-1	gf ⁴/gf ⁴	本课题组标记检测 Our marker detection results
Ailsa Craig	SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SP5G^TS30M/SP5G^TS30M	Lin et al.，2014
Heinz 1706	I-7/I-7、ovate/ovate、PT^R/PT^R、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、sp/sp、SP5G^TS30M/SP5G^TS30M	Sol Genomics Network
Indigo Rose	Aft/Aft、PT^R/PT^R、SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、sp/sp、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results
LA1589	Frl/Frl、Pto/Pto、Rx4/Rx4、SlHAK20^Hap1/SlHAK20^Hap1、SlSOS2^Hap2/SlSOS2^Hap2、Sm/Sm、STP1^Insertion/STP1^Insertion	Sol Genomics Network
M82	PT^R/PT^R、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、Sm/Sm、sp/sp、SP5G^TS30M/SP5G^TS30M	Lin et al.，2014
Moneymaker	I-7/I-7、SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results
Rio Grande	PT^R/PT^R、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、sp/sp、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results
SL116	I-7/I-7	本课题组重测序 Our resequencing results
SL126	y^1794A/y^1794A	本课题组重测序 Our resequencing results
TH43	Bwr-12/Bwr-12、lc/lc、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、SlSOS2^Hap2/SlSOS2^Hap2、SP5G^TS30M/SP5G^TS30M、Ty-1/Ty-1	本课题组重测序 Our resequencing results
VFNT Cherry	Bwr-12/Bwr-12、I-7/I-7、SD1^TK/SD1^TK、SlACR11A^G/SlACR11A^G、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SP5G^TS30M/SP5G^TS30M、STP1^Insertion/STP1^Insertion	本课题组重测序 Our resequencing results
ZS768	Bwr-12/Bwr-12、SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SlSOS2^Hap2/SlSOS2^Hap2、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results

品系 Line	基因型 Genotype	基因型数据来源 Source of genotypic information
15N60	SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SlHAK20^Hap1/SlHAK20^Hap1、Sm/Sm、SP5G^TS30M/SP5G^TS30M、Ty-1/Ty-1	本课题组重测序 Our resequencing results
231-13-2	Sm/Sm	公司标记检测 Company marker detection result
231-30-1	-	公司标记检测 Company marker detection result
231-35-2	Frl/Frl	公司标记检测 Company marker detection result
23N1338-1	Aft/Aft、ovate/ovate	本课题组标记检测 Our marker detection results
23N1503	r^PI114490/r^PI114490	本课题组标记检测 Our marker detection results
24N97-1	gf ⁴/gf ⁴	本课题组标记检测 Our marker detection results
Ailsa Craig	SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SP5G^TS30M/SP5G^TS30M	Lin et al.，2014
Heinz 1706	I-7/I-7、ovate/ovate、PT^R/PT^R、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、sp/sp、SP5G^TS30M/SP5G^TS30M	Sol Genomics Network
Indigo Rose	Aft/Aft、PT^R/PT^R、SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、sp/sp、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results
LA1589	Frl/Frl、Pto/Pto、Rx4/Rx4、SlHAK20^Hap1/SlHAK20^Hap1、SlSOS2^Hap2/SlSOS2^Hap2、Sm/Sm、STP1^Insertion/STP1^Insertion	Sol Genomics Network
M82	PT^R/PT^R、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、Sm/Sm、sp/sp、SP5G^TS30M/SP5G^TS30M	Lin et al.，2014
Moneymaker	I-7/I-7、SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results
Rio Grande	PT^R/PT^R、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、sp/sp、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results
SL116	I-7/I-7	本课题组重测序 Our resequencing results
SL126	y^1794A/y^1794A	本课题组重测序 Our resequencing results
TH43	Bwr-12/Bwr-12、lc/lc、SD1^TK/SD1^TK、SlBBX31^Hap1/SlBBX31^Hap1、SlSOS2^Hap2/SlSOS2^Hap2、SP5G^TS30M/SP5G^TS30M、Ty-1/Ty-1	本课题组重测序 Our resequencing results
VFNT Cherry	Bwr-12/Bwr-12、I-7/I-7、SD1^TK/SD1^TK、SlACR11A^G/SlACR11A^G、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SP5G^TS30M/SP5G^TS30M、STP1^Insertion/STP1^Insertion	本课题组重测序 Our resequencing results
ZS768	Bwr-12/Bwr-12、SD1^TK/SD1^TK、SlALMT9^HMH/SlALMT9^HMH、SlBBX31^Hap1/SlBBX31^Hap1、SlSOS2^Hap2/SlSOS2^Hap2、SP5G^TS30M/SP5G^TS30M	本课题组重测序 Our resequencing results

基因或位点 Gene or locus	优异等位基因或位点 Excellent allelic gene or locus	基因编号 Gene ID	变异类型 Variation type	变异位置 Variation position	参考文献 Reference
Aft（SlAN2-like）	Aft	Solyc10g086290	SNP	基因内部 Intragenic region	Sun et al.,2020
Bwr-12	Bwr-12	无 Without gene ID	SNP	紧密连锁 Tightly linked marker	Kim et al.,2018
Frl	Frl	Solyc09g011610	SNP	基因内部 Intragenic region	李传友等.,2021
GF（SGR）	gf⁴	Solyc08g080090	SNP	基因内部 Intragenic region	Barry & Pandey,2009
I-7	I-7	Solyc08g077740	SNP	基因内部 Intragenic region	Gonzalez-Cendales et al.,2016
LC	lc	Solyc02g083950	SNP	基因内部 Intragenic region	Muños et al.,2011
OVATE	ovate	Solyc02g085500	SNP	基因内部 Intragenic region	Liu et al.,2002
PT	PT^R	Solyc05g054030	SNP	基因内部 Intragenic region	Song et al.,2022
Pto	Pto	Solyc05g013300	SNP	基因内部 Intragenic region	苏晓梅等,2014
R（PSY1）	r^PI114490	Solyc03g031860	SNP	基因内部 Intragenic region	Yuan et al.,2008
Rx4	Rx4	Solyc11g069020	InDel	基因内部 Intragenic region	Pei et al.,2012；Zhang et al.,2021
SD1	SD1^TK	Solyc09g082510	InDel	基因内部 Intragenic region	Ye et al.,2020
SlACR11A	SlACR11A^G	Solyc03g117890	SNP	基因内部 Intragenic region	Song et al.,2021
SlALMT9	SlALMT9^HMH	Solyc06g072910 Solyc06g072920	InDel	基因内部 Intragenic region	Ye et al.,2017
SlBBX31	SlBBX31^Hap1	Solyc07g053140	InDel	基因内部 Intragenic region	Zhu et al.,2023
SlHAK20	SlHAK20^Hap1	Solyc04g008450	InDel	基因内部 Intragenic region	Wang et al.,2020
SlSOS2	SlSOS2^Hap2	Solyc12g009570	InDel	基因内部 Intragenic region	Hong et al.,2023
Sm	Sm	无 Without gene ID	InDel	紧密连锁 Tightly linked marker	Su et al.,2019
SP	sp	Solyc06g074350	SNP	基因内部 Intragenic region	Pnueli et al.,1998
SP5G	SP5G^TS30M	Solyc05g053850	InDel	基因内部 Intragenic region	Zhang et al.,2018
STP1	STP1^Insertion	Solyc02g079220	InDel	基因内部 Intragenic region	Wang et al.,2023
Ty-1	Ty-1	Solyc06g051170 Solyc06g051180 Solyc06g051190	InDel	基因内部 Intragenic region	Verlaan et al.,2013
Y（SlMYB12）	y^1794A	Solyc01g079620	SNP	基因内部 Intragenic region	Jung et al.,2017

基因或位点 Gene or locus	优异等位基因或位点 Excellent allelic gene or locus	基因编号 Gene ID	变异类型 Variation type	变异位置 Variation position	参考文献 Reference
Aft（SlAN2-like）	Aft	Solyc10g086290	SNP	基因内部 Intragenic region	Sun et al.,2020
Bwr-12	Bwr-12	无 Without gene ID	SNP	紧密连锁 Tightly linked marker	Kim et al.,2018
Frl	Frl	Solyc09g011610	SNP	基因内部 Intragenic region	李传友等.,2021
GF（SGR）	gf⁴	Solyc08g080090	SNP	基因内部 Intragenic region	Barry & Pandey,2009
I-7	I-7	Solyc08g077740	SNP	基因内部 Intragenic region	Gonzalez-Cendales et al.,2016
LC	lc	Solyc02g083950	SNP	基因内部 Intragenic region	Muños et al.,2011
OVATE	ovate	Solyc02g085500	SNP	基因内部 Intragenic region	Liu et al.,2002
PT	PT^R	Solyc05g054030	SNP	基因内部 Intragenic region	Song et al.,2022
Pto	Pto	Solyc05g013300	SNP	基因内部 Intragenic region	苏晓梅等,2014
R（PSY1）	r^PI114490	Solyc03g031860	SNP	基因内部 Intragenic region	Yuan et al.,2008
Rx4	Rx4	Solyc11g069020	InDel	基因内部 Intragenic region	Pei et al.,2012；Zhang et al.,2021
SD1	SD1^TK	Solyc09g082510	InDel	基因内部 Intragenic region	Ye et al.,2020
SlACR11A	SlACR11A^G	Solyc03g117890	SNP	基因内部 Intragenic region	Song et al.,2021
SlALMT9	SlALMT9^HMH	Solyc06g072910 Solyc06g072920	InDel	基因内部 Intragenic region	Ye et al.,2017
SlBBX31	SlBBX31^Hap1	Solyc07g053140	InDel	基因内部 Intragenic region	Zhu et al.,2023
SlHAK20	SlHAK20^Hap1	Solyc04g008450	InDel	基因内部 Intragenic region	Wang et al.,2020
SlSOS2	SlSOS2^Hap2	Solyc12g009570	InDel	基因内部 Intragenic region	Hong et al.,2023
Sm	Sm	无 Without gene ID	InDel	紧密连锁 Tightly linked marker	Su et al.,2019
SP	sp	Solyc06g074350	SNP	基因内部 Intragenic region	Pnueli et al.,1998
SP5G	SP5G^TS30M	Solyc05g053850	InDel	基因内部 Intragenic region	Zhang et al.,2018
STP1	STP1^Insertion	Solyc02g079220	InDel	基因内部 Intragenic region	Wang et al.,2023
Ty-1	Ty-1	Solyc06g051170 Solyc06g051180 Solyc06g051190	InDel	基因内部 Intragenic region	Verlaan et al.,2013
Y（SlMYB12）	y^1794A	Solyc01g079620	SNP	基因内部 Intragenic region	Jung et al.,2017

标记名称 Marker ID	等位基因 Allele	SNP或InDel SNP or InDel	PCR产物大小/bp PCR product size	引物序列（5′-3′） Primer sequence
Aft-HRM	Aft	G	160	F：CTTCACAAGCTTCTAGGCA
Aft-HRM	aft	A	160	R：ATGGCATCAAAGATAACTTACTA
Bwr-12-HRM	Bwr-12	G	130	F：CTTCCTTCAAGGTCCACTAC
Bwr-12-HRM	bwr-12	A	130	R：CAACACCGTTAGATTAGCC
Frl-HRM	Frl	G	139	F：CAGATGTTAAGTTGCTTGGTCTA
Frl-HRM	frl	A	139	R：GGATTGGATTCGAGAAGTAGAGA
GF-HRM	gf⁴	T	97	F：AAAGTTGCCAAGAACATATACA
GF-HRM	GF	C	97	R：TTTGATTACCTGAGAATTATTGATG
I-7-HRM	I-7	C	152	F：ATTGGGAGATAATCCGTCG
I-7-HRM	i-7	T	152	R：GGTTCAAACGCCAAAGAG
LC-HRM	lc	C、G	161	F：TTTGAGATGGAGATTTAGAC
LC-HRM	LC	T、A	161	R：GGAATGCTATGAAATGTAT
OVATE-HRM	ovate	T	149	F：GTGGATGGGAAAGTGAAGG
OVATE-HRM	OVATE	G	149	R：GACAGAAAACATTGTAAAAGC
PT-HRM	PT^R	G	90	F：GGTGATCTTGTTGGAATCA
PT-HRM	PT^H	A	90	R：AGCATAACCTTTGTTGCA
Pto-HRM	Pto	A	82	F：AATCTGGAGAAGCGTCAC
Pto-HRM	pto	G	82	R：GAGAAAAGCCATCATGAGCT
R-HRM	r^PI114490	G	121	F：GCTCATTAGTTAGTCGTTAGAGA
R-HRM	R	A	121	R：CGTCATAAATTTGTTCAGGTG
Rx4-HRM	Rx4	6-bp deletion	182	F：GCTTAACCCTCCACATCAA
Rx4-HRM	rx4		188	R：GTATCCCAGCTACCATAACAA
SD1-HRM	SD1^TK		192	F：ATATCTGCCGCGACTAGA
SD1-HRM	SD1^TN	11-bp insertion	203	R：ATGGAAATTGTTGCGAGT
SlACR11A-HRM	SlACR11A^G	G	128	F：CTTGAACTGATATGGCTGT
SlACR11A-HRM	SlACR11A^A	A	128	R：ATCCAAACCAAAAGAACC
SlALMT9-HRM	SlALMT9^HMH	3-bp deletion	215	F：ACCGTGTAACCTTGTGAT
SlALMT9-HRM	SlALMT9^LMH		218	R：GATCGTTGATTTCGATAA
SlBBX31-HRM	SlBBX31^Hap1		166	F：AAAAGTCGCCATAACGAA
SlBBX31-HRM	SlBBX31^Hap2	27-bp insertion	193	R：TGAAATGCACCTGAAGAGG
SlHAK20-HRM	SlHAK20^Hap1	6-bp insertion	197	F：CGGACGGCCTGACTTGAC
SlHAK20-HRM	SlHAK20^Hap2		191	R：AAACAGAGCACAAAGTTCCATT
SlSOS2-HRM	SlSOS2^Hap2	53-bp deletion	147	F：AGTTCGTGATGGGATGTT
SlSOS2-HRM	SlSOS2^Hap1		200	R：AAATGATGACCGATGTAAG
Sm-HRM	Sm	18-bp deletion	121	F：CTACACTTTCTCGTTCCCAATG
Sm-HRM	sm		139	R：TCGCCAAACCAATCAAATC
SP-HRM	sp	T	121	F：TCTTCTATTATTGACCTCTCA
SP-HRM	SP	C	121	R：ATGTTGTCTATACCAGTGTA
SP5G-HRM	SP5G^TS30M		157	F：CAATAGGGAAGGTGGTAC
SP5G-HRM	SP5G^TS30C	52-bp insertion	209	R：CTTTCTGAGTTAATAAGCAAT
STP1-HRM	STP1^Insertion	21-bp insertion	157	F：TCACAAGCCTTACATCTT
STP1-HRM	STP1^Deletion		136	R：AGTGTTCTATCCACCACA
Ty-1-HRM	Ty-1	12-bp insertion	106	F：AATGGGTGATCCGTTGAT
Ty-1-HRM	ty-1		94	R：CTGCTCCTTGCAGATTCTAT
Y-HRM	y^1794A	A	99	F：ACCATCGATCTAGTTTCTA
Y-HRM	Y	G	99	R：CATTGTCTGTTCTACCTGA

Development of HRM Molecular Markers for Important Traits in Tomato and Assessment of Analytical Effects Across Various Reaction Systems

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分子标记Marker	15N60	Rio Grande	VFNT Cherry	TH43	ZS768	MoneyMaker	Indigo Rose	Ailsa Craig	M82	Heinz 1706	LA1589
Aft-HRM	－	－	－	－	－	－	＋	－	－	－	－
Bwr-12-HRM	－	－	＋	＋	＋	－	－	－	－	－	N
Frl-HRM	－	－	－	－	－	－	－	－	－	－	＋
GF-HRM	－	－	－	－	－	－	－	－	－	－	－
I-7-HRM	－	－	＋	－	－	＋	－	－	－	＋	－
LC-HRM	－	－	－	＋	－	－	N	－	－	－	－
OVATE-HRM	－	－	－	－	－	－	－	－	－	＋	－
PT-HRM	－	＋	－	－	－	－	＋	－	＋	＋	－
Pto-HRM	－	－	－	－	－	－	－	－	－	－	＋
R-HRM	－	－	－	－	－	－	－	－	－	－	－
Rx4-HRM	－	－	－	－	－	－	－	－	－	－	＋
SD1-HRM	＋	＋	＋	＋	＋	＋	＋	＋	＋	＋	－
SlACR11A-HRM	－	－	＋	－	－	－	－	－	－	－	－
SlALMT9-HRM	＋	－	＋	－	＋	＋	＋	＋	－	－	N
SlBBX31-HRM	＋	＋	＋	＋	＋	＋	＋	＋	＋	＋	－
SlHAK20-HRM	＋	－	－	－	－	－	－	－	－	－	＋
SlSOS2-HRM	－	－	－	＋	＋	－	－	－	－	－	＋
Sm-HRM	＋	－	－	－	－	－	－	－	＋	－	＋
SP-HRM	－	＋	－	－	－	－	＋	－	＋	＋	－
SP5G-HRM	＋	＋	＋	＋	＋	＋	＋	＋	＋	＋	－
STP1-HRM	－	－	＋	/	－	－	/	－	－	－	＋
Ty-1-HRM	＋	－	－	/	－	－	－	－	－	－	－
Y-HRM	－	－	－	－	－	－	－	－	－	－	－

分子标记Marker	15N60	Rio Grande	VFNT Cherry	TH43	ZS768	MoneyMaker	Indigo Rose	Ailsa Craig	M82	Heinz 1706	LA1589
Aft-HRM	－	－	－	－	－	－	＋	－	－	－	－
Bwr-12-HRM	－	－	＋	＋	＋	－	－	－	－	－	N
Frl-HRM	－	－	－	－	－	－	－	－	－	－	＋
GF-HRM	－	－	－	－	－	－	－	－	－	－	－
I-7-HRM	－	－	＋	－	－	＋	－	－	－	＋	－
LC-HRM	－	－	－	＋	－	－	N	－	－	－	－
OVATE-HRM	－	－	－	－	－	－	－	－	－	＋	－
PT-HRM	－	＋	－	－	－	－	＋	－	＋	＋	－
Pto-HRM	－	－	－	－	－	－	－	－	－	－	＋
R-HRM	－	－	－	－	－	－	－	－	－	－	－
Rx4-HRM	－	－	－	－	－	－	－	－	－	－	＋
SD1-HRM	＋	＋	＋	＋	＋	＋	＋	＋	＋	＋	－
SlACR11A-HRM	－	－	＋	－	－	－	－	－	－	－	－
SlALMT9-HRM	＋	－	＋	－	＋	＋	＋	＋	－	－	N
SlBBX31-HRM	＋	＋	＋	＋	＋	＋	＋	＋	＋	＋	－
SlHAK20-HRM	＋	－	－	－	－	－	－	－	－	－	＋
SlSOS2-HRM	－	－	－	＋	＋	－	－	－	－	－	＋
Sm-HRM	＋	－	－	－	－	－	－	－	＋	－	＋
SP-HRM	－	＋	－	－	－	－	＋	－	＋	＋	－
SP5G-HRM	＋	＋	＋	＋	＋	＋	＋	＋	＋	＋	－
STP1-HRM	－	－	＋	/	－	－	/	－	－	－	＋
Ty-1-HRM	＋	－	－	/	－	－	－	－	－	－	－
Y-HRM	－	－	－	－	－	－	－	－	－	－	－