Studies on the Efficiency of GRFs/GIFs for Genetic Transformation and Regeneration in Grapevine

doi:10.16420/j.issn.0513-353x.2024-0077

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 51-65.doi: 10.16420/j.issn.0513-353x.2024-0077

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Studies on the Efficiency of GRFs/GIFs for Genetic Transformation and Regeneration in Grapevine

LI Min, LI Siyu, SHI Zihan, CHEN Shuang, XU Yan^**(), LIU Guotian^**()

Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China，Ministry of Agriculture and Rural Affairs，College of Horticulture，Northwest A & F University，Yangling，Shaanxi 712100，China

Received:2024-09-19 Revised:2024-11-15 Online:2025-01-25 Published:2025-01-18
Contact: XU Yan, LIU Guotian

Abstract

Abstract:

In order to study the effect of GRF/GIF family genes on the efficiency of genetic transformation and regeneration，10 VvGRF and 4 VvGIF genes were identified in grape genome. VvGRF contains family conserved domains WRC and QLQ，VvGIF contains conserved domains SNH and QG. The regeneration of tobacco overexpressing VvGRF and VvGIF after one month of cultivation was significantly better than that the empty control. VvGRF8-GIF2 and VvGRF4-GIF2 are overexpressed in tobacco，and the regeneration efficiency is as high as 73.58% and 65.05%，which is 2.4-2.7 times that of control. Overexpression of rVvGRF8-GIF2（VvGRF8-GIF2 after mutation of miR396 binding target site）showed the best regeneration efficiency，reaching 83.43%，which was 3.1 times that of control. Compared with the overexpression transformation of VvGRF8-GIF2 chimera，the promotion effect of constructed VvGRF8，VvGIF2 homologous chimera VvGRF4-GIF2，VvGRF5-GIF2 and VvGRF8-GIF3 after overexpression is relatively weak. According to the experimental results，VvGRF8 and VvGIF2 can improve the regeneration efficiency of plant genetic transformation.

Key words: grape, GRF, GIF, genetic transformation, regeneration

LI Min, LI Siyu, SHI Zihan, CHEN Shuang, XU Yan, LIU Guotian. Studies on the Efficiency of GRFs/GIFs for Genetic Transformation and Regeneration in Grapevine[J]. Acta Horticulturae Sinica, 2025, 52(1): 51-65.

Figures/Tables 10

References 48

[1]	Alam I, Wu X, Ge L. 2022. Comprehensive genomic survey,evolution,and expression analysis of GIF gene family during the development and metal ion stress responses in soybean. Plants, 11 (4):570.
[2]	Bailey T L, Boden M, Buske F A, Frith M, Grant C E, Clementi L, Ren J, Li W W, Noble W S. 2009. MEME SUITE:tools for motif discovery and searching. Nucleic Acids Research, 37 (s2):W202-W208.
[3]	Bao M, Bian H, Zha Y, Li F, Sun Y, Bai B, Chen Z, Wang J, Zhu M, Han N. 2014. miR396a-mediated basic helix-loop-helix transcription factor bHLH 74 repression acts as a regulator for root growth in Arabidopsis seedlings. Plant and Cell Physiology, 55 (7):1343-1353.
[4]	Bull T, Debernardi J, Reeves M, Hill T, Bertier L, van Deynze A, Michelmore R. 2023. GRF-GIF chimeric proteins enhance in vitro regeneration and Agrobacterium-mediated transformation efficiencies of lettuce(Lactuca spp.). Plant Cell Reports, 42:629-643.
[5]	Chen C, Chen H, Zhang Y. 2020. An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1194-1202.
[6]	Chen J, Tomes S, Gleave A P, Hall W, Luo Z, Xu J, Yao J L. 2022. Significant improvement of apple(Malus domestica Borkh.)transgenic plant production by pre-transformation with a Baby boom transcription factor. Horticulture Research, 9:uhab014.
[7]	Chen X, Zhang J, Wang S, Cai H, Yang M, Dong Y. 2024. Genome-wide molecular evolution analysis of the GRF and GIF gene families in plantae(Archaeplastida). BMC Genomics, 25:74.
[8]	Debernardi J M, Mecchia M A, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez R E, Palatnik J F. 2014. Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF coactivator affects leaf size and longevity. The Plant Journal, 79:413-426. doi: 10.1111/tpj.12567 pmid: 24888433
[9]	Debernardi J M, Tricoli D M, Ercoli M F, Hayta S, Ronald P, Palatnik J F, Dubcovsky J. 2020. A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nature Biotechnology, 38:1274-1279. doi: 10.1038/s41587-020-0703-0 pmid: 33046875
[10]	Feng Q, Xiao L, He Y, Liu M, Wang J, Tian S, Zhang X, Yuan L. 2021. Highly efficient,genotype-independent transformation and gene editing in watermelon(Citrullus lanatus.)using a chimeric ClGRF4-GIF1 gene. Journal of Integrative Plant Biology, 63:2038-2042.
[11]	Fu M K, He Y N, Yang X Y. 2024. Genome-wide identification of the GRF family in sweet orange(Citrus sinensis)and functional analysis of the CsGRF04 in response to multiple abiotic stresses. BMC Genomics,(2024):25-37.
[12]	Horiguchi G, Ferjani A, Fujikura U, Tsukaya H. 2005a. Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana. Journal of Plant Research, 119:37-42.
[13]	Horiguchi G, Kim G T, Tsukaya H. 2005b. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. The Plant Journal, 43:68-78.
[14]	Hu Q, Jiang B, Wang L, Song Y, Tang X, Zhao Y, Fan X, Gu Y, Zheng Q, Cheng J, Zhang H. 2023. Genome-wide analysis of growth-regulating factor genes in grape(Vitis vinifera L.):identification,characterization and their responsive expression to osmotic stress. Plant Cell Rep, 42 (1):107-121.
[15]	Katayama-Ikegami A, Katayama T, Takai M, Sakamoto T. 2016. Reference gene validation for gene expression studies using quantitative RT-PCR during berry development of‘Aki Queen’grapes. Vitis:Journal of Grapevine Research, 4:157-160.
[16]	Kim J H, Kende H. 2004. A transcriptional coactivator,AtGIF1,is involved in regulating leaf growth and morphology in Arabidopsis. The Proceedings of the National Academy of Sciences, 101:13374-13379.
[17]	Kim J H, Lee B H. 2006. Growth-regulating factor 4 of Arabidopsis thaliana is required for development of leaves,cotyledons,and shoot apical meristem. Jounal of Plant Biology, 49 (6):463-468.
[18]	Kim J S, Mizoi J, Kidokoro S, Maruyama K, Nakajima J, Nakashima K, Mitsuda N, Takiguchi Y, Ohme-Takagi M, Kondou Y, Yoshizumi T, Matsui M, Shinozaki K, Yamaguchi-Shinozaki K. 2012. Arabidopsis growth-regulating factor7 functions as a transcriptional repressor of abscisic acid-and osmotic stress-responsive genes,including DREB2A. the Plant Cell, 24:3393-3405.
[19]	Koichiro T, Glen S, Sudhir K. 2021. MEGA11:Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38 (7):3022-3027. doi: 10.1093/molbev/msab120 pmid: 33892491
[20]	Kong J, Martin-Ortigosa S, Finer J, Orchard N, Gunadi A, Batts L.A, Thakare D, Rush B, Schmitz O, Stuiver M, Olhoft P, Pacheco-Villalobos D. 2020. Overexpression of the transcription factor growth-regulating factor 5 improves transformation of dicot and monocot species. Frontiers in Plant Science,doi:10.3389/fpls.2020.572319.
[21]	Lee S J, Lee B H, Jung J H, Park S K, Song J T, Kim J H. 2018. Growth-regulating factor and GRF-interacting factor specify meristematic cells of gynoecia and anthers. Plant Physiology, 176:717-729.
[22]	Li S, Gao F, Xie K, Zeng X, Cao Y, Zeng J, He Z, Ren Y, Li W, Deng Q, Wang S, Zheng A, Zhu J, Liu H, Wang L, Li P. 2016. The OsmiR396c-OsGRF4-OsGIF1 regulatory module determines grain size and yield in rice. Plant Biotechnol J, 14:2134-2146. doi: 10.1111/pbi.12569 pmid: 27107174
[23]	Li S, Tian Y, Wu K, Ye Y, Yu J, Zhang J, Liu Q, Hu M, Li H, Tong Y, Harberd N P, Fu X. 2018. Modulating plant growth-metabolism coordination for sustainable agriculture. Nature, 560:595-600.
[24]	Li Z, Jiao Y, Zhang C, Dou M, Weng K, Wang Y, Xu Y. 2021. VvHDZ 28 positively regulate salicylic acid biosynthesis during seed abortion in Thompson Seedless. Plant Biotechnology Journal, 19:1824-1838.
[25]	Liang Chen, Sun Yiru, Xiang Rui, Sun Yimeng, Shi Xiaoxin, Du Guoqiang, Wang Li. 2022. Identification and expression analysis of GRF family genes involved in grapevine growth regulation. Acta Horticulturae Sinica, 49 (5):995-1007. (in Chinese)
	梁晨, 孙如意, 向锐, 孙艺萌, 师校欣, 杜国强, 王莉. 2022. 葡萄生长调控因子GRF家族基因的鉴定及表达分析. 园艺学报, 49 (5):995-1007. doi: 10.16420/j.issn.0513-353x.2021-0533 URL
[26]	Liang G, He H, Li Y, Wang F, Yu D. 2013. Molecular mechanism of microRNA396 mediating pistil development in Arabidopsis. Plant Physiology, 164:249-258.
[27]	Liu D, Song Y, Chen Z, Yu D. 2009. Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis. Physiologia Plantarum, 136:223-236.
[28]	Liu H, Guo S, Xu Y, Li C, Zhang Z, Zhang D, Xu S, Zhang C, Chong K.. 2014. OsmiR396d-Regulated OsGRFs function in floral organogenesis in rice through binding to their targets OsJMJ706 and OsCR4. Plant Physiology, 165:160-174. doi: 10.1104/pp.114.235564 pmid: 24596329
[29]	Liu Z, Li N, Zhang Y., Li Y. 2020. Transcriptional repression of GIF1 by the KIX-PPD-MYC repressor complex controls seed size in Arabidopsis. Nature Communications,doi:10.1038/s41467-020-15603-3.
[30]	Lowe K, Wu E, Wang N, Hoerster G, Hastings C, Cho M. J, Scelonge C, Lenderts B, Chamberlin M, Cushatt J, Wang L, Ryan L, Khan T, Chow-Yiu J, Hua W, Yu M, Banh J, Bao Z, Brink K, Igo E, Rudrappa B, Shamseer P M, Bruce W, Newman L, Shen B, Zheng P, Bidney D, Falco C, Register J, Zhao Z Y, Xu D, Jones T, Gordon-Kamm W. 2016. Morphogenic regulators Baby Boom and Wuschel improve monocot transformation. the Plant Cell, 28:1998-2015.
[31]	Lu L, Holt A, Chen X, Liu Y, Knauer S, Tucker E J, Sarkar A K, Hao Z, Roodbarkelari F, Shi J, Chen J, Laux T. 2023. miR394 enhances WUSCHEL-induced somatic embryogenesis in Arabidopsis thaliana. New Phytol, 238 (3):1059-1072. doi: 10.1111/nph.18801 pmid: 36751948
[32]	Luo G, Palmgren M. 2021. GRF-GIF chimeras boost plant regeneration. Trends in Plant Science, 26:201-204. doi: 10.1016/j.tplants.2020.12.001 pmid: 33349565
[33]	Luo X, Zhang Y, Zhou M, Liu K, Zhang S, Ye D, Tang C, Cao J. 2024. Overexpression of HbGRF4 or HbGRF4-HbGIF1 chimera improves the efficiency of somatic embryogenesis in Hevea brasiliensis. Int J Mol Sci, 25:2921.
[34]	Ma J, Li Z, Liu Y. 2023. Integrating multi-omics analysis reveals the regulatory mechanisms of white-violet mutant flowers in grape hyacinth (Muscari latifolium). International Journal of Molecular Sciences, 24:5044.
[35]	McFarland F L, Collier R, Walter N, Martinell B, Kaeppler S M, Kaeppler H F. 2023. A key to totipotency:Wuschel-like homeobox 2a unlocks embryogenic culture response in maize(Zea mays L.). Plant Biotechnology Journal, 21:1860-1872. doi: 10.1111/pbi.14098 pmid: 37357571
[36]	Ogura N, Sasagawa Y, Ito T, Tameshige T, Kawai S, Sano M, Doll Y, Iwase A, Kawamura A, Suzuki T, Nikaido I, Sugimoto K, Ikeuchi M. 2023. Wuschel-related homeobox 13 suppresses de novo shoot regeneration via cell fate control of pluripotent callus. Sci Adv, 9 (27):6983.
[37]	Omidbakhshfard M A, Proost S, Fujikura U, Bernd Mueller-Roeber. 2015. Growth-regulating factors(GRFs):a small transcription factor family with important functions in plant biology. Mol Plant, 8 (7):998-1010. doi: 10.1016/j.molp.2015.01.013 pmid: 25620770
[38]	Pan W, Cheng Z, Han Z, Yang H, Zhang W, Zhang H. 2022. Efficient genetic transformation and CRISPR/Cas9-mediated genome editing of watermelon assisted by genes encoding developmental regulators. Journal of Zhejiang University(Science B), 23:339-344.
[39]	Rodriguez R E, Ercoli M F, Debernardi J M, Breakfield N W, Mecchia M A, Sabatini M, Cools T, De Veylder L, Benfey P N, Palatnik J F. 2015. MicroRNA miR396 regulates the switch between stem cells and transit-amplifying cells in Arabidopsis roots. the Plant Cell, 27:3354-3366. doi: 10.1105/tpc.15.00452 pmid: 26645252
[40]	van der Knaap E, Kim J H, Kende H. 2000. A novel gibberellin-induced gene from rice and its potential regulatory role in stem growth. Plant Physiology, 122 (3):695-704. doi: 10.1104/pp.122.3.695 pmid: 10712532
[41]	Vercruyssen L, Tognetti V B, Gonzalez N, van Dingenen J, de Milde L, Bielach A, de Rycke R, van Breusegem F, Inzé D. 2015. GROWTH-REGULATING FACTOR5 stimulates Arabidopsis chloroplast division,photosynthesis,and leaf longevity. Plant Physiology, 167:817-832. doi: 10.1104/pp.114.256180 pmid: 25604530
[42]	Xiao Ling. 2023. Establishment of highly efficient genetic transformation method independent of genotype in watermelon using ClGRF4-GIF1 [M. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	肖玲. 2023. ClGRF4-GIF1介导的基因型不依赖的西瓜高效遗传转化方法建立[硕士论文]. 杨凌: 西北农林科技大学.
[43]	Zhang B, Tong Y, Luo K Zhai Z, Liu X, Shi Z, Zhang D, Li D. 2021. Identification of Growth-regulating Factor transcription factors in lettuce (Lactuca sativa)genome and functional analysis of LsaGRF 5 in leaf size regulation. BMC Plant Biol, 21:485. doi: 10.1186/s12870-021-03261-6 pmid: 34688264
[44]	Zeng Jia, Yao Kena, Zhang Yixin, Lu Yuzhu. 2022. Progress in the study of GRF transcription factors regulating plant development and regeneration efficiency. Molecular Breeding, http://kns.cnki.net/kcms/detail/46.1068.S.20220517.1124.010.html. (in Chinese)
	曾加, 姚科娜, 张亦心, 鲁玉柱. 2022. GRF转录因子调控植物发育和再生效率研究进展. 分子植物育种, http://kns.cnki.net/kcms/detail/46.1068.S.20220517.1124.010.html.
[45]	Zhang Ying. 2021. Establishment of somatic embryo regeneration system based on‘Chardonnay’grape bud and seed and research on Agrobacterium-mediated gene transformation[M. D. Dissertation]. Yinchuan: Ningxia University. (in Chinese)
	张莹. 2021. 基于‘霞多丽’葡萄花蕾与种子的体细胞胚再生体系建立及农杆菌介导基因转化的研究[硕士论文]. 银川: 宁夏大学.
[46]	Zhao Fengxia, Gao Xiangbin, Wang Zhengping, Li Haifeng, Sun Huihui, Song Xueli. 2014. Progress in the application of genetic engineering in grape research. Chinese Agricultural Science Bulletin, 30 (22):92-96. (in Chinese)
	赵凤霞, 高相彬, 王正平, 李海峰, 孙卉卉, 宋学立. 2014. 基因工程在葡萄研究中的应用进展. 中国农学通报, 30 (22):92-96.
[47]	Zhao Huixia, Li Fengxia, Guo Rui, Chen Chanyou. 2020. Genome-wide identification and expression analysis of GRF gene family in Capsicum. Acta Horticultuae Sinica, 47 (11):2145-2160. (in Chinese)
	赵慧霞, 李凤霞, 郭瑞, 陈禅友. 2020. 辣椒属GRF基因家族全基因组鉴定和表达分析. 园艺学报, 47 (11):2145-2160.
[48]	Zhou R N, Zhao Y J, Cheng P, Zhang B, Liu Z, Wang S, Li H, Chen Q, Zhao Y, Li S, Wu X. 2023. GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean(Glycine max). Biotechnology & Biotechnological Equipment,https://doi.org/10.1080/13102818.2023.2238833.

基因 Gene	基因ID Gene ID	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
VvGRF1	Vitvi02g00239	CCTGGTGGCGTCTATGAGTG	AGCCGCCATTCCTCCAGT
VvGRF2	Vitvi02g00449	CTTCCAAGATTGCCCGCCTT	GCAGCCAAGCATATCCCCAG
VvGRF3	Vitvi08g01498	AGCATTATCAGCCTGCTTTGTTG	TGACCAGTTACCACGTCCCT
VvGRF4	Vitvi09g00107	CCTATCACCTCAGGCCACCA	TCCACACCCTCCCTCATTCC
VvGRF5	Vitvi11g00092	AGGCCATTTTACCTTGCTGGC	AGCTCTCTGCCCCCATTCAC
VvGRF6	Vitvi15g00815	CCGCTTCGGTGTATTGCTTCT	CTGCTCAAGCTCACGCAACT
VvGRF7	Vitvi15g04517	CCATTCGGATTGTCGGGCTC	GCTCAGGGTCAGTGTTTCCG
VvGRF8	Vitvi16g04017	GGTCCGGAACCAACCAATCC	AGGCCATGTGCTGTCCAAAG
VvGRF9	Vitvi16g01354	TGTGGTAAGGTGGTGGGTGT	TCCCACGTTGTTGCAGTGTC
VvGRF10	Vitvi18g00623	CTGGTCAGCAAGAACAGCAG	AAGTTCCCAGAGCTGTAACCTGT
VvGIF1	Vitvi07g00563	TGTTCACTTTGCAGTTTGCAGC	ATCAGTGGTGACGTTGCTGG
VvGIF2	Vitvi14g02674	CATTTTGGACCACCACGGTAGA	TGGTATGCTAACTCCACACGC
VvGIF3	Vitvi15g00903	ATGCAGCAGAACCCCCAGAT	GCTGAGCTTGGTACTGTGC
VvGIF4	Vitvi16g01357	CCTTCATTTCCTCCCACCGC	GGTGGTTGGGCATCAGCAAT
GAPDH	F6GSG7_VITVI	CCAAGGCTGGAATAGCACTC	CCATGTGGACAATCAAGTCG

基因 Gene	基因ID Gene ID	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
VvGRF1	Vitvi02g00239	CCTGGTGGCGTCTATGAGTG	AGCCGCCATTCCTCCAGT
VvGRF2	Vitvi02g00449	CTTCCAAGATTGCCCGCCTT	GCAGCCAAGCATATCCCCAG
VvGRF3	Vitvi08g01498	AGCATTATCAGCCTGCTTTGTTG	TGACCAGTTACCACGTCCCT
VvGRF4	Vitvi09g00107	CCTATCACCTCAGGCCACCA	TCCACACCCTCCCTCATTCC
VvGRF5	Vitvi11g00092	AGGCCATTTTACCTTGCTGGC	AGCTCTCTGCCCCCATTCAC
VvGRF6	Vitvi15g00815	CCGCTTCGGTGTATTGCTTCT	CTGCTCAAGCTCACGCAACT
VvGRF7	Vitvi15g04517	CCATTCGGATTGTCGGGCTC	GCTCAGGGTCAGTGTTTCCG
VvGRF8	Vitvi16g04017	GGTCCGGAACCAACCAATCC	AGGCCATGTGCTGTCCAAAG
VvGRF9	Vitvi16g01354	TGTGGTAAGGTGGTGGGTGT	TCCCACGTTGTTGCAGTGTC
VvGRF10	Vitvi18g00623	CTGGTCAGCAAGAACAGCAG	AAGTTCCCAGAGCTGTAACCTGT
VvGIF1	Vitvi07g00563	TGTTCACTTTGCAGTTTGCAGC	ATCAGTGGTGACGTTGCTGG
VvGIF2	Vitvi14g02674	CATTTTGGACCACCACGGTAGA	TGGTATGCTAACTCCACACGC
VvGIF3	Vitvi15g00903	ATGCAGCAGAACCCCCAGAT	GCTGAGCTTGGTACTGTGC
VvGIF4	Vitvi16g01357	CCTTCATTTCCTCCCACCGC	GGTGGTTGGGCATCAGCAAT
GAPDH	F6GSG7_VITVI	CCAAGGCTGGAATAGCACTC	CCATGTGGACAATCAAGTCG

基因ID Gene locus ID	染色体 Chromosome	基因名称 Gene name	起始与终止位点/bp Start and end position	大小/bp Size	CDS/bp	ORF/aa
Vitvi02g00239	2	VvGRF1	2 139 069 ~ 2 141 255	1 986	1 608	535
Vitvi02g00449	2	VvGRF2	4 219 207 ~ 4 220 305	1 098	882	211
Vitvi08g01498	8	VvGRF3	17 729 492 ~ 17 731 764	2 272	1 191	396
Vitvi09g00107	9	VvGRF4	1 134 269 ~ 1 136 573	2 304	1 998	363
Vitvi11g00092	11	VvGRF5	1 000 319 ~ 1 001 707	1 388	1 164	387
Vitvi15g00815	15	VvGRF6	17 227 979 ~ 17 232 031	4 092	1 838	467
Vitvi15g04517	15	VvGRF7	18 612 801 ~ 18 615 995	3 194	1 794	597
Vitvi16g00073	16	VvGRF8	971 916 ~ 975 816	3 900	1 367	330
Vitvi16g01354	16	VvGRF9	22 127 734 ~ 22 130 159	2 425	1 731	576
Vitvi18g00623	18	VvGRF10	7 058 736 ~ 7 061 990	3 254	1 805	599

基因ID Gene locus ID	染色体 Chromosome	基因名称 Gene name	起始与终止位点/bp Start and end position	大小/bp Size	CDS/bp	ORF/aa
Vitvi02g00239	2	VvGRF1	2 139 069 ~ 2 141 255	1 986	1 608	535
Vitvi02g00449	2	VvGRF2	4 219 207 ~ 4 220 305	1 098	882	211
Vitvi08g01498	8	VvGRF3	17 729 492 ~ 17 731 764	2 272	1 191	396
Vitvi09g00107	9	VvGRF4	1 134 269 ~ 1 136 573	2 304	1 998	363
Vitvi11g00092	11	VvGRF5	1 000 319 ~ 1 001 707	1 388	1 164	387
Vitvi15g00815	15	VvGRF6	17 227 979 ~ 17 232 031	4 092	1 838	467
Vitvi15g04517	15	VvGRF7	18 612 801 ~ 18 615 995	3 194	1 794	597
Vitvi16g00073	16	VvGRF8	971 916 ~ 975 816	3 900	1 367	330
Vitvi16g01354	16	VvGRF9	22 127 734 ~ 22 130 159	2 425	1 731	576
Vitvi18g00623	18	VvGRF10	7 058 736 ~ 7 061 990	3 254	1 805	599

基因ID Gene locus ID	染色体 chromosome	基因名称 Gene name	起始与终止位点/bp Start and end position	大小/bp Size	CDS/bp	ORF/aa
Vitvi07g00563	7	VvGIF1	6 086 919 ~ 6 092 810	5 891	1 024	224
Vitvi14g02674	14	VvGIF2	7 536 676 ~ 7 559 301	22 625	1 037	223
Vitvi15g00903	15	VvGIF3	18 236 759 ~ 18 241 761	5 002	1 159	222
Vitvi16g01357	16	VvGIF4	22 146 392 ~ 22 148 829	2 437	597	189

Studies on the Efficiency of GRFs/GIFs for Genetic Transformation and Regeneration in Grapevine

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 48

Related Articles 15

Recommended Articles

Metrics

Comments

过表达载体 Overexpressed vector	外植体数 Number of explants	出芽数 Bud number	平均再生效率/% Mean regeneration efficiency	过表达载体 Overexpressed vector	外植体数 Number of explants	出芽数 Bud number	平均再生效率/% Mean regeneration efficiency
GFP	26	7	26.83 ± 1.03 e	rVvGRF8-GIF2	40	35	83.43 ± 4.39 a
VvGRF8	31	14	43.29 ± 1.09 d		43	33
	30	13			43	37
	29	12		VvGRF5-GIF2	18	9	44.44 ± 3.21 d
VvGIF2	30	11	40.00 ± 1.36 d		18	8
	30	13			18	7
	30	12		VvGRF4-GIF2	22	13	65.05 ± 3.39 b
VvGRF8+GIF2	31	16	53.57 ± 5.03 c		23	15
	31	15			24	17
	28	17		VvGRF8-GIF3	15	7	45.69 ± 0.97 cd
VvGRF8-GIF2	24	17	73.58 ± 2.11 ab		15	7
	23	17			16	7
	25	19

[1]	ZHAO Yulei, LI Shan, LI Chengnan, MA Jinlong, MA Hongyi, and YIN Xiao. Proteomics Studies on the Early Stages of Botrytis cinerea Infection in Grapevine Leaves [J]. Acta Horticulturae Sinica, 2025, 52(2): 279-291.
[2]	CHEN Yajuan, JIN Xin, YANG Jiangshan, DAI Zibo, LI Dou, and SHAO Zhang. Effects of Fulvic Acid Potassium on Sugar-Acid Metabolism and Aroma Substances in‘Cabernet Gernischt’Grape Berries [J]. Acta Horticulturae Sinica, 2025, 52(2): 406-422.
[3]	LI Xiaoqing, YAN Siyuan, and GU Peiwen. Diversity and Pathogenicity Differentiation of Botrytis cinerea in Ningxia [J]. Acta Horticulturae Sinica, 2025, 52(2): 481-490.
[4]	WANG Jianzhao, GAO Yuan, DAO Mei, ZHANG Hongye, YANG Ziyun, CHEN Longqing, WU Tian. Establishment of Regeneration in Vitro System of Camellia japonica and Relationship Between Endogenous Hormones and Adventitious Bud Differentiation [J]. Acta Horticulturae Sinica, 2024, 51(8): 1891-1905.
[5]	YANG Jinghui, XU Yuan, XIAO Ting, CHU Shupin, LIU Jixiang, YAO Kebing. Resistance of Colletotrichum gloeosporioides Species Complexes from Grape to Azoxystrobin [J]. Acta Horticulturae Sinica, 2024, 51(8): 1906-1912.
[6]	HAN Bin, LIU Changjiang, YIN Yonggang, SUN Yan, JIA Nan, ZHAO Shengjian, GUO Zijuan, LI Minmin. A New Medium and Late Ripening Table Grape Cultivar‘Jinguang’ [J]. Acta Horticulturae Sinica, 2024, 51(8): 1977-1978.
[7]	LIU Miaomiao, YAO Jin, BAO Min, CHU Yanyan, WANG Xiping. Research Progress on the Mechanism of GAs-Induced Seedlessness in Grape [J]. Acta Horticulturae Sinica, 2024, 51(7): 1610-1622.
[8]	MA Zonghuan, LI Yumei, WEI Xiaxia, LI Wenfang, CHEN Baihong, MAO Juan. Analysis of Quality and Aroma Components of‘Merlot’Grape from Different Areas in Hexi Corridor [J]. Acta Horticulturae Sinica, 2024, 51(5): 1083-1098.
[9]	LI Dou, WANG Yuhang, WANG Chunheng, JIN Xin, YANG Jiangshan, CHEN Yajuan, DAI Zibo, and FENG Lidan. Effects of Exogenous GABA on Physiological Characteristics of Leaves and Fruit Flavor in Wine Grape [J]. Acta Horticulturae Sinica, 2024, 51(4): 815-831.
[10]	DU Yijing, LIU Wenlin, QIAO Yuelian, WANG Li, AN Dezhi, DU Guoqiang, SHI Xiaoxin. Virus Elimination from‘Shine Muscat’Grape Plantlets in vitro via Heat Treatment Combined with Shoot Tip and Axillary Bud Culture [J]. Acta Horticulturae Sinica, 2024, 51(4): 893-902.
[11]	YI Qian, ZHANG Manman, WANG Xiaoli, FENG Jipeng, ZHU Shiping, WANG Fusheng, ZHAO Xiaochun. CclSAUR49 Affects Growth and Limonoids Metabolism in Citrus [J]. Acta Horticulturae Sinica, 2024, 51(3): 479-494.
[12]	XIA Hongyi, LIU Qiao, PENG Jiaqing, WU Wei, GONG Linzhong. Effects of f-Shaped Tree Shape on Photosynthetic Characteristics and Fruit Quality in‘Shine Muscat’Grapevines with Rain-Shelter Cultivation [J]. Acta Horticulturae Sinica, 2024, 51(3): 560-570.
[13]	WANG Haixia, JIANG Yaping, FANG Yan, YANG Xueshan, ZHU Xia. Effect of Exogenous Water-Soluble β-Glucans Application on Glycosidase Activity and Aroma Quality of‘Italian Riesling’Grape [J]. Acta Horticulturae Sinica, 2024, 51(3): 571-586.
[14]	YANG Xue, HU Jinhong, LI Jingjing, ZHANG Yingcai, WANG Lingxia, LIANG Wenyu. Genetic Transformation of Lycium Barbarum TGA2 Gene in Arabidopsis Enhances Its Salt Tolerance [J]. Acta Horticulturae Sinica, 2024, 51(12): 2829-2842.
[15]	YANG Jiangshan, CHEN Yajuan, DAI Zibo, LI Dou, SHAO Zhang, JIN Xin, WANG Yuhang, WANG Chunheng. Effects of Potassium Fulvic Acid on Photosynthetic Characteristics and Fruit Quality of‘Cabernet Gernischt’Grape [J]. Acta Horticulturae Sinica, 2024, 51(12): 2843-2856.