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Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (1): 1-24.doi: 10.16420/j.issn.0513-353x.2024-0564

• Genetic & Breeding·Germplasm Resources·Molecular Biology •     Next Articles

Genome-Wide Identification of CYP71 Subfamily Genes in Citrus sinensis and Functional Analysis of CsCYP2

WANG Hong1, WAN Yunqing1, QI Xinai1, SHENG Huizi1, REN Yalin1, LIU Mengyu1, LIU Xiaofeng1,*(), ZHAO Xiaochun1,2,3,*()   

  1. 1 National Citrus Engineering Research CenterCitrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, China
    2 Yibin Academy of Southwest UniversityYibin, Sichuan 644005, China
    3 Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City, Chongqing 401329, China
  • Received:2025-06-04 Revised:2025-10-24 Online:2026-01-25 Published:2026-01-26
  • Contact: LIU Xiaofeng, ZHAO Xiaochun

Abstract:

The CYP71 gene family is the largest subfamily within the cytochrome P450 enzyme family and plays a crucial role in the biosynthesis pathways of secondary metabolites such as terpenes and flavonoids. Specifically,it is involved in the hydroxylation and oxidation modifications of terpenes,closely associated with the production of plant metabolite derivatives. In the sweet orange genome,138 CYP71 genes have been identified,with highly conserved motifs and gene structures among its members. Promoter cis-acting element analysis revealed that the CsCYP71 subfamily contains light-responsive,hormone-responsive,and other regulatory elements. Through transcriptomic,gene expression,and volatile component analyses,the candidate gene Cs3g07330(termed as CsCYP2)was identified as significantly correlated with essential oil content. The expression level of this gene in leaves and peels showed a strong positive correlation with essential oil accumulation,and it was predominantly expressed in oil glands,suggesting its potential involvement in essential oil biosynthesis. In transgenic plants with CsCYP2 overexpression and gene editing,the expression of CsCYP2 was found to be highly positively correlated with the content of the dioxygenated monoterpene 2-hydroxycineole(exo-2-hydroxycineole). This indicates that CsCYP2 may be a key gene responsible for the oxidative modification in the synthesis of exo-2-hydroxycineole.

Key words: citrus, CYP71 subfamily gene, oxidative modification, essential oil, terpene derivatives