Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana

doi:10.16420/j.issn.0513-353x.2024-0224

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (1): 149-158.doi: 10.16420/j.issn.0513-353x.2024-0224

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana

FU Shuai¹^,², XU Liping², WANG ZhouPing², YE Wei³, XU Han¹^,⁴, YE Kaiwen¹^,⁵, ZHANG Jiao⁶, LIN Yuling¹^,^*, LAI Zhongxiong¹^,^*()

¹ Institute of Horticultural Plant Bioengineering， Fujian Agriculture and Forestry University， Fuzhou 350002， China
² Shaanxi Horticultural Technology Workstation， Xi’an 710000， China
³ Sanming Agricultural Sciences Research Institute， Sanming， Fujian 365000， China
⁴ Institute of Comprehensive Science in Toulouse， France， Toulouse 31000， France
⁵ Institute of Plant Science， Taiwan University， Taipei 10617， China
⁶ Xianyang Academy of Agricultural Sciences， Xianyang， Shaanxi 712000， China

Received:2025-05-16 Revised:2025-12-01 Online:2026-01-25 Published:2026-01-26
Contact: LIN Yuling, LAI Zhongxiong

Abstract

Abstract:

By analyzing the protein sequences，conserved domains，and physicochemical properties of OnMYB106 from Oncidium（Honey Angel）and AtMYB106 from Arabidopsis thaliana，it is speculated that the functions of the two are similar. The mutant of the AtMYB106（myb106）was used as the research object，and it was genetically transformed with OnMYB106（35S:OnMYB106-myb106）. The phenotypes days to bolting、number of rosette leaves and pod-setting of the wild type，myb106 and 35S: OnMYB106-myb106 were comparatively analyzed. The results showed that the bolting times for the wild-type，myb106 and 35S:OnMYB106-myb106 were 32，28 and 31 d，respectively；the number of rosette leaves at bolting was 12，8 and 12 pcs，respectively；and the rosette leaf canopy diameters at bolting were 4.1，2.8 and 3.5 cm，respectively. The number of siliques per plant was 64，15 and 42 siliques，respectively. The wild-type siliques contained approximately 19 seeds each and were about 1.07 cm in length. These values were significantly lower in the myb106，with approximately 7 seeds and 0.75 cm length，respectively. The 35S:OnMYB106-myb106 complementation line showed values of 18 seeds and 0.81 cm. Overall，the silique production capacity of the three lines，ranked from highest to lowest，was：wild-type > 35S:OnMYB106-myb106 > myb106.

Key words: Oncidium, Arabidopsis, OnMYB106, fertility

FU Shuai, XU Liping, WANG ZhouPing, YE Wei, XU Han, YE Kaiwen, ZHANG Jiao, LIN Yuling, LAI Zhongxiong. Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana[J]. Acta Horticulturae Sinica, 2026, 53(1): 149-158.

Figures/Tables 9

Table 1 Primers information used in this study

引物名称 Primer name	引物序列（5′-3′） Primer sequence	退火温度/℃ Annealing temperature
AtMYB106-LP	ATACTCACTCTCATTCCCCACG	60
AtMYB106-RP	GCTCCTCAACAACATTGCTTC	60
BP	GCGTGGACCGCTTGCTGCAACT	60
Q-GUS-F	CGACTGGGCAGATGAACAT	60
Q-GUS-R	ATACTCCACATCACCACGCT	60
RT-OnMYB106-F	GAAGAGCTGTAGGTTGAGGTGGACGA	60
RT-OnMYB106-R	CGGGTCAATCCCCATCTTTGC	58
RT-AtMYB106-F	ACCAAGGAAACGGAGACCAAC	60
RT-AtMYB106-R	ATCTGCCTAATCCAGTCGTGTT	60
Actin-F	GCAACATTGTTCTTAGCGGAGGCT	60
Actin-R	TCTTCATGCTGCTTGGTGCAAGTG	60

Fig. 1 Prediction of conserved protein domains，nuclear localization signal structure（A） and Motif analysis（B）of Oncidium and Arabidopsis thaliana On：Oncidium；At：Arabidopsis thaliana

Fig. 2 Analysis of AtMYB106 gene structure and insertion site（A）and identification results（B，C）

Fig. 3 GUS detection of myb106 mutant overexpressing OnMYB106 plants 1-8：GUS detection bands of transgenic positive plants

Fig. 4 Analysis of the expression levels of AtMYB106 in myb106（A）and OnMYB106 in 35S:OnMYB106-myb106（B）

Fig. 5 Phenotypic analysis of wild type，myb106 and 35S-OnMYB106-myb106 mutant in Arabidopsis thaliana

Fig. 6 Phenotypic analysis of wild-type（WT），myb106 and 35S:OnMYB106-myb106 Arabidopsis at bolting

Table 2 Analysis of silique of wild type，myb106 and 35S:OnMYB106-myb106

基因型 Genotype	果荚数 Number of per silique	种子数 Number of seeds	果荚长/cm Silique length
野生型Wild type	64.08 ± 7.85	19.91 ± 6.03	1.12 ± 0.09
myb106	12.00 ± 3.00**	7.00 ± 2.00**	0.75 ± 0.19**
35S: OnMYB106-myb106	42.25 ± 7.90*	18.16 ± 1.82	0.81 ± 0.90*

Fig. 7 Alexander staining of anthers and pollen grains in Arabidopsis during anther opening Arrows indicate aborted pollen grains

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Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana

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