Isolation，Identification and Functional Analysis of Symbiotic Bacteria of Fusarium oxysporum f. sp. cucumerium

doi:10.16420/j.issn.0513-353x.2023-0001

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (3): 643-655.doi: 10.16420/j.issn.0513-353x.2023-0001

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Isolation，Identification and Functional Analysis of Symbiotic Bacteria of Fusarium oxysporum f. sp. cucumerium

LIU Meng, JIA Huiting, ZHOU Xingang^*()

College of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin 150030，China

Received:2023-04-29 Revised:2023-12-04 Online:2024-03-25 Published:2024-03-22
Contact: ZHOU Xingang

Abstract

Abstract:

In this experiment，Fusarium oxysporum f. sp. cucumerium was used as the research object. The presence of symbiotic bacteria outside and inside the hyphae of F. oxysporum f. sp. cucumerium was determined by fluorescent nucleic acid staining and electron microscopy. The symbiotic bacteria were successfully isolated by plate culture method and dentified by 16S rDNA sequencing. It was determined that it belonged to Achromobacter spp. The analysis of carbon source utilization showed that compared with F. oxysporum f. sp. cucumerium without symbiotic bacteria，the Shannon index and anti-Simpson index of F. oxysporum f. sp. cucumerium with symbiotic bacteria were significantly increased by 2.18% and 1.67% respectively，and the utilization ability of sugars，carboxylic acids，amines，polymers and other carbon sources was strong，while the utilization ability of amino acids was weak. The results showed that there was symbiotic bacteria of Achromobacter spp. in F. oxysporum f. sp. cucumerium，and it may affect the growth and metabolic capacity of the host fungus.

Key words: cucumber, Fusarium oxysporum f. sp. cucumerium, symbiotic bacteria, isolation and identification, carbon source utilization

LIU Meng, JIA Huiting, ZHOU Xingang. Isolation，Identification and Functional Analysis of Symbiotic Bacteria of Fusarium oxysporum f. sp. cucumerium[J]. Acta Horticulturae Sinica, 2024, 51(3): 643-655.

Figures/Tables 10

References 41

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编号 No．	对应克隆子数 The number of clones	NCBI数据库中最相似菌种（登录号） The strains which have the highest identity from NCBI（Accession No. ）	相似度/% Similarity	系统分类 Phylogenetic classification
OTU1	20	耐氧梭菌Clostridium aerotolerans DSM 5434（T）（X76163）	99.72	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU2	17	解木聚糖梭菌Clostridium xylanolyticum ATCC 49623（T）（X71855）	99.66	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU3	9	耐氧梭菌Clostridium aerotolerans DSM 5434（T）（X76163）	99.65	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU4	1	约氏不动杆菌Acinetobacter johnsonii CIP 64.6（T）（APON01000005）	98.42	变形菌门Proteobacteria，γ‐变形菌纲Gammaproteobacteria假单胞菌目Pseudomonadales，莫拉氏菌科Moraxellaceae，不动杆菌属Acinetobacter
OTU5	1	解木聚糖梭菌Clostridium xylanolyticum ATCC 49623（T）（X71855）	99.24	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU6	1	耐氧梭菌Clostridium aerotolerans DSM 5434（T）（X76163）	99.30	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU7	1	马德普拉塔无色杆菌 Achromobacter marplatensis B2（T）（EU150134）	99.78	变形菌门Proteobacteria，β‐变形菌纲Betaproteobacteria，伯克氏菌目Burkholderiales，产碱菌科Alcaligenaceae，无色杆菌属Achromobacter

编号 No．	对应克隆子数 The number of clones	NCBI数据库中最相似菌种（登录号） The strains which have the highest identity from NCBI（Accession No. ）	相似度/% Similarity	系统分类 Phylogenetic classification
OTU1	20	耐氧梭菌Clostridium aerotolerans DSM 5434（T）（X76163）	99.72	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU2	17	解木聚糖梭菌Clostridium xylanolyticum ATCC 49623（T）（X71855）	99.66	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU3	9	耐氧梭菌Clostridium aerotolerans DSM 5434（T）（X76163）	99.65	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU4	1	约氏不动杆菌Acinetobacter johnsonii CIP 64.6（T）（APON01000005）	98.42	变形菌门Proteobacteria，γ‐变形菌纲Gammaproteobacteria假单胞菌目Pseudomonadales，莫拉氏菌科Moraxellaceae，不动杆菌属Acinetobacter
OTU5	1	解木聚糖梭菌Clostridium xylanolyticum ATCC 49623（T）（X71855）	99.24	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU6	1	耐氧梭菌Clostridium aerotolerans DSM 5434（T）（X76163）	99.30	厚壁菌门Firmicutes，梭菌纲Clostridia，梭菌目Clostridiales，梭菌科Clostridiaceae，梭菌属Clostridium
OTU7	1	马德普拉塔无色杆菌 Achromobacter marplatensis B2（T）（EU150134）	99.78	变形菌门Proteobacteria，β‐变形菌纲Betaproteobacteria，伯克氏菌目Burkholderiales，产碱菌科Alcaligenaceae，无色杆菌属Achromobacter

菌株 Strain	碳源利用 Carbon source utilization	Shannon指数 H	反Simpson指数 D
FOC	0.983 ± 0.0595 a	4.304 ± 0.0100 a	0.488 ± 0.0014 a
FOC⁻	0.901 ± 0.0495 b	4.212 ± 0.0122 b	0.480 ± 0.0016 b

菌株 Strain	碳源利用 Carbon source utilization	Shannon指数 H	反Simpson指数 D
FOC	0.983 ± 0.0595 a	4.304 ± 0.0100 a	0.488 ± 0.0014 a
FOC⁻	0.901 ± 0.0495 b	4.212 ± 0.0122 b	0.480 ± 0.0016 b

Isolation，Identification and Functional Analysis of Symbiotic Bacteria of Fusarium oxysporum f. sp. cucumerium

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