Determination of Horizontal Transmission Ability of BdCV1 Infecting Botryosphaeria dothidea Strain with Hypovirulence

doi:10.16420/j.issn.0513-353x.2022-0683

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2477-2488.doi: 10.16420/j.issn.0513-353x.2022-0683

• Plant Protection • Previous Articles Next Articles

Determination of Horizontal Transmission Ability of BdCV1 Infecting Botryosphaeria dothidea Strain with Hypovirulence

GAO Yunjing, WANG Tianhao, HU Wangcheng, HONG Ni, WANG Guoping, WANG Liping^*()

College of Plant Science and Technology，Huazhong Agricultural University，Key Laboratory of Plant Pathology of Hubei Province，Wuhan 430070，China

Received:2023-03-11 Revised:2023-06-28 Online:2023-11-25 Published:2023-11-28
Contact: WANG Liping

Abstract

Abstract:

Pear ring rot disease，caused by the pathogenic agent of Botryosphaeria dothidea. Botryosphaeria dothidea chrysovirus 1（BdCV1）induced to the hypovirulence of B. dothidea LW-C and LW-1，which will be used as an ideal candidate material for biological control of fruit tree ring rot disease. In order to further clarify whether the BdCV1-mediated hypovirulent strain has potential as a good candidate biocontrol resource，LW-C and LW-1 was confrontly cultured with the B. dothidea strains isolated and collected from different geographical origins and Valsa pyri strain XJ-28，which caused serious harm to pear trees，respectively. The obtained derivate strains were detected to be positive for BdCV1，whose growth rates and pathogenicity were further determined and analyzed. The results showed that BdCV1 could be successfully horizontally transmitted to different B. dothidea and V. pyri strains with certain frequencies，which further inhibited the growth rates and induced to hypovirulence of the strains. The obtained results revealed BdCV1 could be successfully transmitted to the recipient strains at a certain frequency，which could cause the growth rate to decrease and inhibit the growth of the recipient strains to different extent. The formed lesion extension lengths of derivate strains with BdCV1 range from about two to four times longer than that of the recipient strains，when they are inoculated with pear branches or fruits. It further demonstrated that BdCV1 can inhibit the virulence of recipient strains. In summary，BdCV1 could be horizontally transmitted to different recipient strains，revealing that it has wide host range. It is predicted that BdCV1 could be used as a potential biocontrol factor in pear fungal disease biocontrol in practice.

Key words: pear, Botryosphaeria dothidea, Valsa pyri, Botryosphaeria dothidea chrysovirus 1 （BdCV1）, horizontal transmission, biological control

GAO Yunjing, WANG Tianhao, HU Wangcheng, HONG Ni, WANG Guoping, WANG Liping. Determination of Horizontal Transmission Ability of BdCV1 Infecting Botryosphaeria dothidea Strain with Hypovirulence[J]. Acta Horticulturae Sinica, 2023, 50(11): 2477-2488.

Figures/Tables 10

Table 1 The information of recipient strains used for confrontation culture

病原种类 Pathogen species	菌株编号 Strains number	采集地 Collection place	寄主品种 Host species	枝干症状 Branches symptom
梨轮纹病菌 Botryosphaeria dothidea	HB-2	河北 Hebei	雪青 Xueqing	轮纹 Ring rot
	LN128	辽宁大连 Dalian，Liaoning	道拉恩道梨 Daolaendao pear	干腐、溃疡 Dry rot，ulcer
	HB216	湖北武汉 Wuhan，Hubei	砂梨‘丰水’ Pyrus pyrifolia ‘Housi’	轮纹 Ring rot
梨腐烂病菌Valsa pyri	XJ-28	新疆库尔勒 Korla，Xinjiang	库尔勒香梨 Korla pear	树皮腐烂、黄色卷丝状孢子角 Bark decay，yellow filamentous spore horns

Fig. 1 The schematic diagram of the horizontal transmission of BdCV1 by confrontation culture of donor strains and recipient strains on PDA or OMA Black dots：Donor strain；Blue dots：Recipient strain；Red pentagram：Derivates.

Fig. 2 The colony morphologies of strains from horizontal transmission of BdCV1 to different Botrytosperia dothidea isolates Ⅰ：Colony growth of LW-C and recipient strains cultured on OMA for six days；Ⅱ：Colony morphologies of LW-C and recipient strains in confrontation culture on OMA for six days；Ⅲ：Colony morphologies of derivates cultured on PDA for six days. The last digits such as LW-C/HB-2-1 and LW-C/HB-2-3 represent replicates one and three in the four sets of replicates.

Fig. 3 The dsRNA profiles detection by 1.2% agarose gel electrophoresis（A）and growth rate determination（B）of derived strains Different lowercase letters indicate significant differences among different treatments at 0.05 level. The same below.

Fig. 4 Inoculation of recipient and derivate strains on‘Huangguan’pear fruit for five days induced to lesion symptoms

Fig. 5 The lesion diameter of‘Huangguan’pear fruit at five days after inoculation with the recipient and derivate strains

Fig. 6 Horizontal transmission test results of BdCV1 in Valsa pyri XJ-28 stain Ⅰ：Colony morphologies of LW-1 and recipient strain XJ-28 cultured on PDA for six days and five days，respectively；Ⅱ：Colony morphologies of LW-1 and recipient strain XJ-28 in confrontation culture on PDA for five days；Ⅲ：Colony morphologies of derivates cultured on PDA for three days.

Fig. 7 Derived strain dsRNA electrophoresis（A）and RT-PCR electrophoresis（B）results of BdCV1 in Valsa pyri XJ-28 stain

Fig. 8 Growth rate determination of XJ-28 and three derivate strains

Fig. 9 Inoculation of recipient strain XJ-28 and derivates on‘Hongxiangsu’pear branches exhibited lesion symptoms（A） and lesion extension lengths in diameter were determined for seven days（B） Ⅰ：Branches disease symptom of PDA；Ⅱ：Branches disease symptom of recipient strain XJ-28；III：Branches disease symptoms of derived strains.

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Determination of Horizontal Transmission Ability of BdCV1 Infecting Botryosphaeria dothidea Strain with Hypovirulence

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