Screening and Identification of Antagonistic Bacteria Against Gray Leaf Spot of Tomato Caused by Stemphylium solani

doi:10.16420/j.issn.0513-353x.2019-0630

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (4): 741-748.doi: 10.16420/j.issn.0513-353x.2019-0630

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Screening and Identification of Antagonistic Bacteria Against Gray Leaf Spot of Tomato Caused by Stemphylium solani

LI Xinyu1,2，LI Lei1，CHEN Lida1，SHI Yanxia1，CHAI Ali1，XIE Xuewen1,*，and LI Baoju1,*

1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Plant Protection College，Shenyang Agricultural University，Shenyang 110866，China

Online:2020-04-25 Published:2020-04-25

Abstract

Abstract: Gray leaf spot is one of major diseases on tomato. At present，chemical control was the only way against the disease. So it has a significant meaning to screen and use biocontrol bacteria to deal with the disease. A bacterial strain，named ZF161，isolated from rhizosphere soil of tomato in a continuous cropping system，exhibited a high antagonistic effect on gray leaf spot of tomato caused by Stemphylium solani Weber. The plate bioassay showed that the inhibition rate on S. solani was 70.51%. The control effect of ZF161 against tomato gray leaf spot of inoculated tomato detached leaf was 69.83%. The strain was identified as Bacillus subtilis based on morphological，biochemical and physiological analysis. The pot experiment result revealed that the control effect of ZF161 against tomato gray leaf spot was 63.27%. The antifungal activity of strain ZF161 was tested by plate confrontation method. The result showed that strain ZF161 exhibited a broad spectrum against seven pathogenic fungi. Overall，B. subtilis ZF161 is a beneficial bacteria in biocontrol of plant disease.

Key words: tomato, antagonistic bacteria, Bacillus subtilis, gray leaf spot of tomato, control effect

CLC Number:

S 641.2

LI Xinyu1,2，LI Lei1，CHEN Lida1，SHI Yanxia1，CHAI Ali1，XIE Xuewen1,*，and LI Baoju1,*. Screening and Identification of Antagonistic Bacteria Against Gray Leaf Spot of Tomato Caused by Stemphylium solani[J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 741-748.

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Screening and Identification of Antagonistic Bacteria Against Gray Leaf Spot of Tomato Caused by Stemphylium solani

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