黄瓜与酸黄瓜异源多倍体3种同工酶表达基因组剂量效应

园艺学报 ›› 2008, Vol. 35 ›› Issue (3): 377-382.

黄瓜与酸黄瓜异源多倍体3种同工酶表达基因组剂量效应

罗向东^{1, 2}，戴亮芳^{1, 2}，钱春桃¹，娄群峰¹，陈劲枫^1*

（¹作物遗传与种质创新国家重点实验室，南京农业大学园艺学院，南京 210095； ²江西师范大学生命科学学院，南昌 330022）

收稿日期:2007-08-27 修回日期:2007-11-25 出版日期:2008-03-25 发布日期:2008-03-25
通讯作者: 陈劲枫

Genome Dosage Effect on Three Kinds of Isozyme Expression Patterns in Cucumis

LUO Xiang-dong^{1, 2}, DAI Liang-fang^{1, 2}, QIAN Chun-tao¹, LOU Qun-feng¹, and CHEN Jin-feng^1*

（¹National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095 China; ²College of Life Science, Jiang xi Normal University, Nanchang 330022, China）

Received:2007-08-27 Revised:2007-11-25 Online:2008-03-25 Published:2008-03-25
Contact: CHEN Jin-feng

摘要/Abstract

摘要：

以甜瓜属（Cucumis）黄瓜与酸黄瓜的3种不同倍性异源多倍体 - 双单倍体种间杂种F₁（2n = 2x = 19，HC）、异源三倍体黄瓜（2n = 3x = 26，HCC）和异源四倍体C. hytivus (2n = 4x = 38，HHCC)及其双亲为试材，比较研究过氧化物酶（POD）、谷氨酸脱氢酶（GDH）和天冬氨酸转氨酶（AAT）在不同倍性异源多倍体中的酶谱表达特性，探讨基因组剂量对酶谱表达的影响。结果表明：不同倍性异源多倍体的酶带染色力表现出明显的差异，但酶带数和酶带分布基本一致。在AAT同工酶中，酶带的表达随基因组剂量的提高而增强，在POD和GDH同工酶中，大部分酶带的表达随基因组剂量的提高而减弱。这些结果表明甜瓜属异源多倍体在不同的酶系统中，酶谱表达可能会表现出截然相反的基因组剂量效应。本研究结果可为

关键词: 甜瓜属, 同工酶, 黄瓜, 基因组剂量, 异源多倍体

Abstract:

Three alloploids with different ploidies from interspecific hybridization in Cucumis [interspecific hybrids F1 (2n = 2x = 19, HC), allotriploid (2n = 3x = 26，HCC), and allotetraploid C. hytivus (2n = 4x = 38，HHCC)] and their parents were used for the comparative studies on expression pattern of peroxidase (POD), glutamate dehydrogenase (GDH), and aspartate aminotransferase (AAT). The results showed that the zymograms in the alloploids were similar in band number and band distribution. The intensity of band in these allopolyploids, however, presents significant difference, which was caused by genome dosage. In AAT zymogram, the intensity of band was positively correlated with ploidy level. In POD and GDH zymogram, however, the intensity of band was negatively correlated with ploidy level. It was indicated that the coherence between genome dosage and isozyme expression pattern would be opposite in different enzyme systems. These data will be helpful to illuminate the morphological changes caused by the polyploid level.

Key words: Cucumis, isozyme, cucumber, genome dosage, allopolyploid

中图分类号:

S 642.2

罗向东;;戴亮芳;;钱春桃;娄群峰;陈劲枫. 黄瓜与酸黄瓜异源多倍体3种同工酶表达基因组剂量效应[J]. 园艺学报, 2008, 35(3): 377-382.

LUO Xiang-dong;;DAI Liang-fang;;QIAN Chun-tao;LOU Qun-feng;and CHEN Jin-feng. Genome Dosage Effect on Three Kinds of Isozyme Expression Patterns in Cucumis [J]. ACTA HORTICULTURAE SINICA, 2008, 35(3): 377-382.

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