抗坏血酸提高月季切花失水胁迫耐性与增加APX活性的关系

园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 333-337.

抗坏血酸提高月季切花失水胁迫耐性与增加APX活性的关系

金基石¹;李永红²;单宁伟¹;高俊平^1*

(¹中国农业大学观赏园艺与园林系, 北京100094; ²深圳职业技术学院应用化学与生物技术学院, 深圳518055)

收稿日期:2005-04-06 修回日期:2005-08-27 出版日期:2006-04-25 发布日期:2006-04-25

Ascorbate Acid Increases Tolerance to Water Deficit Stress in Flowers of Cut Roses ( Rosa hybrida L. ) Caused by Enhanced Ascorbate Peroxidase Activity

Jin Jishi¹;Li Yonghong²;Shan Ningwei¹;and Gao Junping^1*

(¹Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, China; ² School of Applied Chemistry and Biological Engineering, Shenzhen Ploytechnics, Shenzhen 518055, China)

Received:2005-04-06 Revised:2005-08-27 Online:2006-04-25 Published:2006-04-25

摘要/Abstract

摘要： 以‘Samantha’品种为试材, 确定了适宜的抗坏血酸(AsA) 预处理浓度, 探讨了AsA和抗坏血酸过氧化物酶(APX) 抑制剂(对氨基酚, β-aminophenol) 预处理对花瓣中相对电导率、AsA含量以及APX活性的影响。与未经失水胁迫的切花月季相比较, 失水胁迫处理抑制切花的开放进程, 缩短了瓶插寿命。AsA 1 000 mg·L^{- 1}预处理可有效地改善花朵的开放状况, 显著降低花瓣中相对电导率的增加, 明显提高花瓣中AsA的含量和APX活性。500 mg·L ^{- 1} β-aminophenol预处理则起到相反的效应。结果说明,AsA对‘Samantha’月季切花失水胁迫耐性的改善与花瓣中APX活性的提高相联系。

关键词: 月季, 切花, 失水胁迫耐性, 抗坏血酸, 抗坏血酸过氧化物酶

Abstract: Pretreatmentwith ascorbic acid (AsA) increased tolerance of the flowers of cut roses to water deficit stress. The op timum p retreatment concentrations of AsA and β-aminophenol ( an inhibitor of ascorbate peroxidase, APX) were determined in cut rose‘Samantha’ flowers. Water deficit stress inhibited flower opening and reduced vase life. Pretreatment of 1 000 mg·L^{- 1} AsA improved markedly the flower opening, delayed significantly the increase in relative electric conductivity, and increased obviously AsA content and
APX activity. The negative resultswere obtained when 500 mg·L^{- 1}β-aminophenol pretreatment was used.These results indicate that the improvement of AsA on tolerance to water deficit stress may be related to enhanced APX activity in petals of cut roses.

Key words: Rose, Cut rose, Tolerance to water deficit stress, Ascorbate acid, Ascorbate peroxidase

金基石;李永红;单宁伟;高俊平. 抗坏血酸提高月季切花失水胁迫耐性与增加APX活性的关系[J]. 园艺学报, 2006, 33(2): 333-337.

Jin Jishi;Li Yonghong;Shan Ningwei;and Gao Junping. Ascorbate Acid Increases Tolerance to Water Deficit Stress in Flowers of Cut Roses ( Rosa hybrida L. ) Caused by Enhanced Ascorbate Peroxidase Activity[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 333-337.

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