https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2021, Vol. 48 ›› Issue (6): 1135-1149.doi: 10.16420/j.issn.0513-353x.2020-0094

• 研究论文 • 上一篇    下一篇

乙烯代谢和能量状态对‘巴茨拉’牡丹切花瓶插品质的作用研究

王依1, 王凯轩1,2,3, 胡思源1, 周爽1, 史国安1,*()   

  1. 1河南科技大学牡丹学院,洛阳市牡丹生物学重点实验室,河南洛阳 471023
    2中国科学院武汉植物园,植物种质创新与特色农业重点实验室,武汉 430074
    3中国科学院大学,北京 100049
  • 收稿日期:2021-02-09 修回日期:2021-03-28 出版日期:2021-06-25 发布日期:2021-07-07
  • 通讯作者: 史国安 E-mail:gashi1963@163.com
  • 基金资助:
    国家重点研发计划项目(2018YFD1000407);国家自然学(31372098);河南省自然科学(162300410075)

Effects of Ethylene Metabolism and Energy Status on Vase Quality of Cut Itoh Peony‘Bartzella’Flowers

WANG Yi1, WANG Kaixuan1,2,3, HU Siyuan1, ZHOU Shuang1, SHI Guoan1,*()   

  1. 1Luoyang Key Laboratory of Peony Biology,College of Tree Peony,Henan University of Science and Technology,Luoyang,Henan 471023,China
    2Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China
    3University of Chinese Academy of Sciences,Beijing 100049,China
  • Received:2021-02-09 Revised:2021-03-28 Online:2021-06-25 Published:2021-07-07
  • Contact: SHI Guoan E-mail:gashi1963@163.com

摘要:

以发育至绽口期的‘巴茨拉’牡丹切花为试材,分别经蒸馏水和20 mg · L-1 纳米银预处理2 h,再进行蒸馏水或保鲜液瓶插,测定瓶插过程中花瓣乙烯释放速率,1-氨基环丙烷-1-羧酸(ACC)、腺苷酸(ANP)和糖含量,乙烯和糖代谢相关酶活性。结果表明,纳米银预处理乙烯释放峰值降幅达40.9%,降低了ACC含量和ACC合成酶(ACS)活性,提高了琥珀酸脱氢酶(SDH)和H+-ATP酶活性,增加了糖和ATP含量,保持较高的能荷(EC)水平,切花瓶插寿命延长了40.7 h。保鲜液瓶插乙烯释放峰值降幅达35.1%,提高了SDH活性与糖及ATP含量,保持较高的EC值,最大花径增加了3.5 cm。纳米银预处理后保鲜液瓶插(NS + PS)乙烯释放峰值降幅达31.2%,降低了ACC含量和ACS活性,提高了SDH、CCO和H+-ATP酶活性,糖及ANP含量,维持了较高的EC水平,提高了可溶性蛋白质含量,瓶插寿命延长了46.4 h,最大花径增大3.6 cm。结果显示通过抑制乙烯代谢和增强能量状态可延缓‘巴茨拉’牡丹切花衰老提高瓶插品质。

关键词: 牡丹, 切花, 乙烯代谢, 能量状态, 瓶插品质

Abstract:

The regulatory effects of ethylene metabolism and energy status on the vase quality of cut Itoh peony‘Bartzella’flowers were studied by using nano silver and preservative solution treatment. Cut Itoh peony flowers with a developmental level of stageⅡwere employed as test materials and were pretreated with distilled water(the control)or 20 mg · L-1 nano silver(NS)for 2 h. The samples were then vased in preservative solution(PS)directly after distilled water treatment or after nano silver treatment (NS + PS). The release rate of ethylene,the contents of 1-aminocyclopropane-1-carboxylic acid(ACC),adenine nucleotides(ATP + ADP + AMP),sugar,and the activities of enzymes related to ethylene and sugar metabolism during the vase insertion process were measured. The results demonstrated that nano-silver pretreatment(NS)inhibited the peak of ethylene release by 40.9%,and reduced ACC content and ACC synthetase(ACS)activity,while increased the activities of succinic dehydrogenase(SDH)and H+-ATPase,and contents of sugar and ATP,which maintained a high energy charge(EC)level,therefore,prolonged vase life by 40.7 h. The PS also inhibited the peak of ethylene release by 35.1%,and increased SDH activity and sugar and ATP contents,which maintained a high EC value,and increased the maximum flower diameter by 3.5 cm. Moreover,NS + PS group showed inhibition of ethylene release peak by 31.2%,and reduction of ACC content and ACS activity,while SDH,CCO,and H+-ATPase activities,and the contents of sugar and ATP were all increased,which also maintained high EC levels and increased the soluble protein content,and therefore,increased flower vase life by 46.4 h,and the maximum flower diameter by 3.6 cm. The results showed that inhibition of ethylene metabolism and enhancement of energy status could delay the senescence and improve the vase quality of Itoh peony‘Bartzella’cut flowers.

Key words: tree peony, cut flower, ethylene metabolism, energy status, vase quality

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