茶树品种资源遗传多样性的AFLP研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 317-322.

茶树品种资源遗传多样性的AFLP研究

黄建安^{1, 2};李家贤³;黄意欢²;罗军武^2*;龚志华²;刘仲华^{1, 2}

(¹教育部茶学重点实验室, 长沙410128; ² 湖南农业大学茶学系, 长沙410128; ³ 广东省农业科学院茶叶研究所, 广东英德513042)

收稿日期:2005-03-21 修回日期:2005-06-20 出版日期:2006-04-25 发布日期:2006-04-25

Genetic Diversity of Tea 〔Camellia sinensis ( L. ) O. Kuntze〕Cultivars Revealed by AFLP Analysis

Huang Jian'an^{1, 2}; Li Jiaxian³;Huang Yihuan²;Luo Junwu^2*;Gong Zhihua²;Liu Zhonghua^{1, 2}

(¹Key Laboratory of Tea Science of Ministry of Education, Changsha 410128, China; ² Tea Science Department, Hunan Agricultural University, Changsha 410128, China; ³ Tea Research Institute of Guangdong, Yingde 513042, Guangdong, China)

Received:2005-03-21 Revised:2005-06-20 Online:2006-04-25 Published:2006-04-25

摘要/Abstract

摘要： 利用AFLP - 银染分子标记技术, 对40个茶树品种(系) 进行遗传多样性与亲缘关系分析。选用多态性高、分辨力强的引物组合E-ACG/M-AAC、E-AGG/M-AAG与E-AGG/M-AGT分别对供试材料的基因组DNA进行扩增, 共获得226条清晰可辨的带, 其中多态性带207条, 多态位点百分率为91.59% ,这表明供试品种资源在DNA水平上酶切位点的分布存在广泛的变异。40份资源所检出的位点平均有效等位基因数、平均基因多样度、平均Shannon信息指数, 分别为1.59 ±0.09、0.35 ±0.04、0.53 ±0.05。应用SPSS软件计算遗传距离介于0.13～0148之间, 平均为0.32; UPGMA法将40份资源分成4个类群, 从相似性系数分析了各品种资源间的亲缘关系。

关键词: 茶树, AFLP, 遗传多样性

Abstract: 40 tea cultivars were analyzed by AFLP (Amplified Fragment Length Polymorphism) -silver staining protocol in order to detect the genetic diversity and relationship. AFLP fingerprinting of 40 tea cultivars with primer combinations E-ACG/M-AAC, E-AGG/M-AAG and E-AGG/M-AGT revealed a total number of 226 unambiguous bands, of which 207 ones were polymorphic and the polymorphism frequency was 91.59%. This result showed the abundant diversities of restriction sites among tea cultivars studied. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei's gene diversity and Shannonpsinformation index were 1.59 ±0.09, 0.35 ±0.04 and 0.53 ±0.05 respectively. As analyzed by SPSS12, the Nei's genetic distances of 40 tea cultivars ranged from 0.13 to 0.48, and the average distance was 0.32.
These tea cultivars were divided into four group s by UPGMA ( unweighted pair group method with arithmetic average) based on Nei's genetic distances. The genetic relationship of 40 tea cultivarswas analyzed using the similarity coefficient.

Key words: Tea plant, AFLP, Genetic diversity

黄建安;;李家贤;黄意欢;罗军武;龚志华;刘仲华;. 茶树品种资源遗传多样性的AFLP研究[J]. 园艺学报, 2006, 33(2): 317-322.

Huang Jian'an;;Li Jiaxian;Huang Yihuan;Luo Junwu;Gong Zhihua;Liu Zhonghua;. Genetic Diversity of Tea 〔Camellia sinensis ( L. ) O. Kuntze〕Cultivars Revealed by AFLP Analysis[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 317-322.

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