关键词: 茶树, 烯醇酶, 基因克隆, 逆境胁迫

Abstract:

The cDNA fragment sequence encoding enolase（ENO）was obtained from the suppression subtractive library between Camellia sinensis‘Huangdan’and Camellia sinensis‘Jinguanyin’，and then the full-length sequence of ENO was cloned and verified from C. sinensis‘Tieguanyin’and named as CsENO（Accession No. KX962311）. The cDNA length of CsENO was 1 753 bp，containing a 1 332 bp open reading frame（ORF）encoded 444 amino acid residues. Amino acid sequence analysis indicated that CsENO had the highly conserved regions in plants with specific structure domains of ENO and had the closer genetic relationship with Malus × domestica and Pyrus bretschneideri，which shared 84% identity in amino acids. Bioinformatic prediction showed that CsENO belonged to the stability and hydrophilic protein with no transmembrane structure and signal peptide. It might be localized in other subcells and there were phosphorylation sites within the polypeptide chain. Secondary structure of CsENO was predicted to be mainly consisted of alpha helix.The quantitative real-time PCR showed that CsENO was all expressed in response to cold stress，ABA，high salinity as well as drought treatment. And its expression was induced by different degrees.

Key words: tea plant, enolase（ENO）, gene cloning, abiotic stress