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园艺学报 ›› 2012, Vol. 39 ›› Issue (7): 1278-.

• 果树 • 上一篇    下一篇

丙烯和1–甲基环丙烯处理对采后柿果实XTH基因表达的影响

祝庆刚, 饶景萍, 田红炎, 韩 叶   

  1. 西北农林科技大学园艺学院,陕西杨凌 712100
  • 出版日期:2012-07-25 发布日期:2012-07-25

Effects of 1-methylcyclopropene and Propylene on Expression of XTH Genes in Persimmon Fruits

 ZHU  Qing-Gang, RAO  Jing-Ping, TIAN  Hong-Yan, HAN   Ye   

  1. College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Online:2012-07-25 Published:2012-07-25

摘要: 为了进一步探索木葡聚糖内糖基转移/水解酶(XTH)在柿(Diospyros kaki L.)采后软化中的分子调控机制,应用实时荧光定量PCR 技术检测常温下丙烯和1–甲基环丙烯(1-MCP)处理的‘富平尖柿’果实中XTH 基因表达量的变化。结果显示,丙烯处理能够促进柿果实成熟软化,增加乙烯释放并使高峰提前到来,而1-MCP 处理能够延缓果实软化,抑制乙烯释放。随着柿果实成熟软化推进,4 个XTH 基因呈现不同的表达方式,且丙烯处理促进了4 个XTH 基因的表达,而1-MCP 处理则抑制它们的表达。其中,DkXTH1 和DkXTH2 在柿果实成熟软化过程中表达量较高,且二者表达高峰分别在果实成熟的初期和中期,说明DkXTH1 和DkXTH2 分别与采后柿果实初期和中后期的软化关系密切。结合乙烯释放量结果分析显示,柿果实XTH 基因的表达受乙烯调控,对柿果实的成熟软化中具有一定的促进作用。

关键词: 柿, 丙烯, 1–甲基环丙烯, XTH, 采后, 实时荧光定量PCR

Abstract: In order to further explore the molecular regulation mechanism of xyloglucan endotransglycosylase/ hydrolase(XTH)during‘Fuping Jianshi’persimmon(Diospyros kaki L.)postharvest softening,the responses of XTH genes in persimmon fruit to propylene and 1-MCP at the room temperature were investigated by real-time quantitative PCR(RT-qPCR)approach. The results showed that treatment with propylene promoted the softening of persimmon fruits,increased the ethylene production and advanced the onset of climactic of ethylene production;While treatment with 1-MCP delayed the
softening of persimmon fruits,and inhibited ethylene production. Accompanying with fruit ripening,four XTHs genes showed different expression patterns. Propylene treatment promoted the expression of four XTHs genes while 1-MCP treatment inhibited the expression of four genes. The expression levels of
DkXTH1 and DkXTH2 were high during persimmon fruit softening,and the expression peaks of two genes were appeared in the early stage and middle stage,respectively. These results indicated that fruit softening was related to both DkXTH1 and DkXTH2 expression in‘Fuping Jianshi’persimmons and DkXTH1 might involve in the early stage while DkXTH2 was associated with the later stage of fruit softening. Therefore, the expression of XTH genes in persimmon fruits was concluded to be regulated by ethylene and was related to persimmon fruit softening.

Key words: persimmon, propylene, 1-MCP, XTH, postharvest, real-time quantitative PCR

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