关键词: 化感, 嫁接, 茄子, 黄萎病, 根际微生物, 拮抗菌

Abstract: The changes of microbial community structure in rhizosphere soil of grafted eggplants at different stages and the effect of grafting on resistance of eggplant were studied. Antagonistic microbes from rhizosphere of grafted eggplants were screened. Microorganism from rhizosphere of eggplants was isolated by soil dilution. Compared with self-root eggplant, grafting changed microbial community structure in rhizosphere of eggplants, with increase of bacteria (B) and actinomyces (A), decrease of fungi (F), enhancement of the ratios of B to F and A to F, and also decrease of disease incidence of Verticillium wilt. Through ordinal determination of a dual culture, the spore germination and a cylinder-plate, 7 strains from 352 strains isolated from rhizosphere of grafted eggplant indicated significant inhibitory effect against Verticillium dahiae. In pot trial, two antagonistic strains of X631 and Z111 showed significantly inhibitory effect against Verticillium dahiae. Through preliminary identification, strain X631 is Serratia Bizio, and strain Z111 is Trichoderma. The results from this study indicated that the rhizosphere microbial community via grafting is favorable to eggplant's resistance and growth.

Key words: allelopathy, grafting, eggplants, Verticillium wilt, rhizosphere microorganism, antagonistic microbes