园艺学报 ›› 2023, Vol. 50 ›› Issue (4): 875-884.doi: 10.16420/j.issn.0513-353x.2021-1220
万映伶1, 金亦佳1, 刘爱青2, 朱梦婷1, 刘燕1,*()
收稿日期:
2022-11-22
修回日期:
2023-02-26
出版日期:
2023-04-25
发布日期:
2023-04-27
通讯作者:
*(E-mail:chblyan@163.com)
E-mail:chblyan@163.com
基金资助:
WAN Yingling1, JIN Yijia1, LIU Aiqing2, ZHU Mengting1, LIU Yan1,*()
Received:
2022-11-22
Revised:
2023-02-26
Online:
2023-04-25
Published:
2023-04-27
Contact:
*(E-mail:chblyan@163.com)
E-mail:chblyan@163.com
摘要:
依据33对SSR荧光引物的扩增结果,从遗传变异和遗传距离角度对中国4个主要栽培区的386个观赏芍药品种的遗传多样性进行评价。结果显示:(1)中国观赏芍药品种资源具有较高遗传多样性,所用SSR引物的多态信息含量(PIC)值介于0.62 ~ 0.84,平均观测杂合度(Ho)和期望杂合度(He)为0.73、0.78,香农信息指数(I)介于1.35 ~ 2.08。(2)各栽培地芍药品种的遗传多样性从高到低依次为山东菏泽、河南洛阳、江苏扬州和甘肃临洮。(3)方差分析显示变异主要来自栽培地内个体间,不同栽培地品种遗传分化系数(FST)为0.031,基因流(Nm)为7.795。(4)各栽培地品种间的遗传距离介于0.032 ~ 0.123,遗传一致性介于0.884 ~ 0.969;主坐标分析和聚类结果显示品种遗传分化情况与栽培地不完全相关。研究表明,中国观赏芍药品种遗传信息丰富,但4个栽培地间遗传分化小,各栽培地间的基因交流强。
中图分类号:
万映伶, 金亦佳, 刘爱青, 朱梦婷, 刘燕. 不同栽培地芍药品种资源遗传多样性的SSR分析比较[J]. 园艺学报, 2023, 50(4): 875-884.
WAN Yingling, JIN Yijia, LIU Aiqing, ZHU Mengting, LIU Yan. Genetic Diversity of Chinese Herbaceous Peony Resources from Various Cultivation Areas Based on SSR Markers[J]. Acta Horticulturae Sinica, 2023, 50(4): 875-884.
位点 Locus | 重复基序 Repeat motif | 产物大小/bp Size range | 等位基因数 Number of observed alleles (Na) | 有效等位 基因数 Number of effective alleles (Ne) | 香农信息指数 Shannon information index (I) | 观测杂合度 Observation heterozygosity (Ho) | 期望杂合度 Expected heterozy- gosity(He) | 固定系数 Fixed index (F) | 多态信息含量 Polymorphism information content (PIC) |
---|---|---|---|---|---|---|---|---|---|
T852 | (AT)8 | 158 ~ 367 | 26 | 3.99 | 1.82 | 0.40 | 0.75 | 0.46 | 0.72 |
S024 | (AAT)16 | 161 ~ 201 | 23 | 4.32 | 1.92 | 0.72 | 0.77 | 0.07 | 0.74 |
TA704 | (TC)17 | 237 ~ 277 | 22 | 4.62 | 1.95 | 0.51 | 0.78 | 0.35 | 0.76 |
TA564 | (TA)8 | 172 ~ 201 | 21 | 5.00 | 1.97 | 0.77 | 0.80 | 0.04 | 0.78 |
S025 | (ATT)7 | 268 ~ 350 | 19 | 5.36 | 2.01 | 0.68 | 0.81 | 0.17 | 0.79 |
TA038 | (CT)9 | 259 ~ 299 | 19 | 5.20 | 2.05 | 0.23 | 0.81 | 0.72 | 0.79 |
TA133 | (AT)8 | 258 ~ 286 | 17 | 4.46 | 1.88 | 0.56 | 0.78 | 0.28 | 0.75 |
T300 | (AT)10 | 218 ~ 238 | 17 | 3.20 | 1.62 | 0.55 | 0.69 | 0.20 | 0.66 |
T125 | (TC)9 | 128 ~ 170 | 15 | 4.79 | 1.78 | 0.86 | 0.79 | -0.08 | 0.76 |
T356 | (AG)9 | 247 ~ 269 | 15 | 4.18 | 1.70 | 0.34 | 0.76 | 0.56 | 0.72 |
TA700 | (AG)9 | 278 ~ 312 | 15 | 4.99 | 1.90 | 0.81 | 0.80 | -0.01 | 0.77 |
T205 | (TC)9 | 275 ~ 301 | 14 | 5.80 | 2.04 | 0.83 | 0.83 | 0.00 | 0.81 |
TA028 | (AC)6 | 101 ~ 123 | 14 | 3.54 | 1.63 | 0.74 | 0.72 | -0.03 | 0.69 |
TA134 | (GA)16 | 142 ~ 224 | 14 | 4.60 | 1.92 | 0.90 | 0.78 | -0.16 | 0.76 |
S237 | (CTG)8 | 193 ~ 219 | 14 | 4.53 | 1.78 | 0.84 | 0.78 | -0.08 | 0.75 |
T160 | (CT)10 | 205 ~ 227 | 13 | 6.91 | 2.08 | 0.82 | 0.86 | 0.04 | 0.84 |
TA464 | (TC)8 | 200 ~ 214 | 13 | 6.23 | 1.95 | 0.80 | 0.84 | 0.05 | 0.82 |
F106 | (TATG)5 | 200 ~ 290 | 13 | 5.31 | 1.84 | 0.76 | 0.81 | 0.06 | 0.79 |
SA010 | (AAT)5 | 264 ~ 294 | 13 | 3.91 | 1.66 | 0.73 | 0.74 | 0.01 | 0.71 |
TA673 | (CA)18 | 190 ~ 288 | 12 | 3.64 | 1.54 | 0.90 | 0.72 | -0.24 | 0.68 |
T241 | (GA)15 | 127 ~ 157 | 12 | 5.75 | 1.95 | 0.90 | 0.83 | -0.08 | 0.81 |
TA074 | (CT)8 | 260 ~ 306 | 12 | 4.22 | 1.73 | 0.89 | 0.76 | -0.17 | 0.73 |
TA082 | (AG)6 | 242 ~ 280 | 11 | 5.03 | 1.74 | 0.72 | 0.80 | 0.10 | 0.77 |
TA610 | (TC)8 | 257 ~ 291 | 11 | 4.42 | 1.67 | 0.79 | 0.77 | -0.02 | 0.74 |
TA086 | (AG)10 | 393 ~ 419 | 10 | 3.04 | 1.39 | 0.63 | 0.67 | 0.05 | 0.62 |
T317 | (CT)10 | 157 ~ 183 | 10 | 5.94 | 1.89 | 0.92 | 0.83 | -0.11 | 0.81 |
T237 | (CT)10 | 95 ~ 109 | 10 | 4.24 | 1.65 | 0.73 | 0.76 | 0.04 | 0.73 |
T040 | (GA)7 | 228 ~ 246 | 9 | 4.53 | 1.66 | 0.71 | 0.78 | 0.09 | 0.75 |
T179 | (AT)7 | 235 ~ 269 | 9 | 3.88 | 1.46 | 0.68 | 0.74 | 0.09 | 0.70 |
T863 | (AG)10 | 140 ~ 160 | 9 | 4.37 | 1.70 | 0.82 | 0.77 | -0.06 | 0.74 |
S853 | (TTG)5 | 158 ~ 186 | 9 | 5.15 | 1.76 | 0.83 | 0.81 | -0.03 | 0.78 |
T304 | (GA)10 | 147 ~ 163 | 8 | 4.45 | 1.67 | 0.87 | 0.78 | -0.12 | 0.74 |
T163 | (TA)8 | 146 ~ 160 | 7 | 2.99 | 1.35 | 0.70 | 0.67 | -0.05 | 0.62 |
均值Mean | 13.82 | 4.62 | 1.78 | 0.73 | 0.78 | 0.06 | 0.75 | ||
标准误SE | 0.80 | 0.16 | 0.03 | 0.03 | 0.01 | 0.04 | 0.01 |
表1 SSR引物在386个芍药品种中的扩增信息
Table 1 Amplification information of SSR primers from 386 herbaceous peony cultivars
位点 Locus | 重复基序 Repeat motif | 产物大小/bp Size range | 等位基因数 Number of observed alleles (Na) | 有效等位 基因数 Number of effective alleles (Ne) | 香农信息指数 Shannon information index (I) | 观测杂合度 Observation heterozygosity (Ho) | 期望杂合度 Expected heterozy- gosity(He) | 固定系数 Fixed index (F) | 多态信息含量 Polymorphism information content (PIC) |
---|---|---|---|---|---|---|---|---|---|
T852 | (AT)8 | 158 ~ 367 | 26 | 3.99 | 1.82 | 0.40 | 0.75 | 0.46 | 0.72 |
S024 | (AAT)16 | 161 ~ 201 | 23 | 4.32 | 1.92 | 0.72 | 0.77 | 0.07 | 0.74 |
TA704 | (TC)17 | 237 ~ 277 | 22 | 4.62 | 1.95 | 0.51 | 0.78 | 0.35 | 0.76 |
TA564 | (TA)8 | 172 ~ 201 | 21 | 5.00 | 1.97 | 0.77 | 0.80 | 0.04 | 0.78 |
S025 | (ATT)7 | 268 ~ 350 | 19 | 5.36 | 2.01 | 0.68 | 0.81 | 0.17 | 0.79 |
TA038 | (CT)9 | 259 ~ 299 | 19 | 5.20 | 2.05 | 0.23 | 0.81 | 0.72 | 0.79 |
TA133 | (AT)8 | 258 ~ 286 | 17 | 4.46 | 1.88 | 0.56 | 0.78 | 0.28 | 0.75 |
T300 | (AT)10 | 218 ~ 238 | 17 | 3.20 | 1.62 | 0.55 | 0.69 | 0.20 | 0.66 |
T125 | (TC)9 | 128 ~ 170 | 15 | 4.79 | 1.78 | 0.86 | 0.79 | -0.08 | 0.76 |
T356 | (AG)9 | 247 ~ 269 | 15 | 4.18 | 1.70 | 0.34 | 0.76 | 0.56 | 0.72 |
TA700 | (AG)9 | 278 ~ 312 | 15 | 4.99 | 1.90 | 0.81 | 0.80 | -0.01 | 0.77 |
T205 | (TC)9 | 275 ~ 301 | 14 | 5.80 | 2.04 | 0.83 | 0.83 | 0.00 | 0.81 |
TA028 | (AC)6 | 101 ~ 123 | 14 | 3.54 | 1.63 | 0.74 | 0.72 | -0.03 | 0.69 |
TA134 | (GA)16 | 142 ~ 224 | 14 | 4.60 | 1.92 | 0.90 | 0.78 | -0.16 | 0.76 |
S237 | (CTG)8 | 193 ~ 219 | 14 | 4.53 | 1.78 | 0.84 | 0.78 | -0.08 | 0.75 |
T160 | (CT)10 | 205 ~ 227 | 13 | 6.91 | 2.08 | 0.82 | 0.86 | 0.04 | 0.84 |
TA464 | (TC)8 | 200 ~ 214 | 13 | 6.23 | 1.95 | 0.80 | 0.84 | 0.05 | 0.82 |
F106 | (TATG)5 | 200 ~ 290 | 13 | 5.31 | 1.84 | 0.76 | 0.81 | 0.06 | 0.79 |
SA010 | (AAT)5 | 264 ~ 294 | 13 | 3.91 | 1.66 | 0.73 | 0.74 | 0.01 | 0.71 |
TA673 | (CA)18 | 190 ~ 288 | 12 | 3.64 | 1.54 | 0.90 | 0.72 | -0.24 | 0.68 |
T241 | (GA)15 | 127 ~ 157 | 12 | 5.75 | 1.95 | 0.90 | 0.83 | -0.08 | 0.81 |
TA074 | (CT)8 | 260 ~ 306 | 12 | 4.22 | 1.73 | 0.89 | 0.76 | -0.17 | 0.73 |
TA082 | (AG)6 | 242 ~ 280 | 11 | 5.03 | 1.74 | 0.72 | 0.80 | 0.10 | 0.77 |
TA610 | (TC)8 | 257 ~ 291 | 11 | 4.42 | 1.67 | 0.79 | 0.77 | -0.02 | 0.74 |
TA086 | (AG)10 | 393 ~ 419 | 10 | 3.04 | 1.39 | 0.63 | 0.67 | 0.05 | 0.62 |
T317 | (CT)10 | 157 ~ 183 | 10 | 5.94 | 1.89 | 0.92 | 0.83 | -0.11 | 0.81 |
T237 | (CT)10 | 95 ~ 109 | 10 | 4.24 | 1.65 | 0.73 | 0.76 | 0.04 | 0.73 |
T040 | (GA)7 | 228 ~ 246 | 9 | 4.53 | 1.66 | 0.71 | 0.78 | 0.09 | 0.75 |
T179 | (AT)7 | 235 ~ 269 | 9 | 3.88 | 1.46 | 0.68 | 0.74 | 0.09 | 0.70 |
T863 | (AG)10 | 140 ~ 160 | 9 | 4.37 | 1.70 | 0.82 | 0.77 | -0.06 | 0.74 |
S853 | (TTG)5 | 158 ~ 186 | 9 | 5.15 | 1.76 | 0.83 | 0.81 | -0.03 | 0.78 |
T304 | (GA)10 | 147 ~ 163 | 8 | 4.45 | 1.67 | 0.87 | 0.78 | -0.12 | 0.74 |
T163 | (TA)8 | 146 ~ 160 | 7 | 2.99 | 1.35 | 0.70 | 0.67 | -0.05 | 0.62 |
均值Mean | 13.82 | 4.62 | 1.78 | 0.73 | 0.78 | 0.06 | 0.75 | ||
标准误SE | 0.80 | 0.16 | 0.03 | 0.03 | 0.01 | 0.04 | 0.01 |
抽样 Sampling | 样本数 Numbers of sampling (N) | 等位基因数 Number of observed alleles (Na) | 有效等位基因数 Number of effective alleles (Ne) | 香农信息指数 Shannon information index (I) | 观测杂合度 Observation heterozygosity (Ho) | 期望杂合度 Expected heterozygosity(He) |
---|---|---|---|---|---|---|
第1次1st | 29.75 ± 0.04 | 6.91 ± 0.15 | 3.98 ± 0.10 | 1.53 ± 0.02 | 0.71 ± 0.02 | 0.73 ± 0.01 |
第2次2nd | 29.75 ± 0.04 | 7.30 ± 0.15 | 4.04 ± 0.09 | 1.57 ± 0.02 | 0.72 ± 0.02 | 0.73 ± 0.01 |
第3次3rd | 29.75 ± 0.04 | 7.32 ± 0.15 | 4.12 ± 0.10 | 1.58 ± 0.02 | 0.71 ± 0.02 | 0.74 ± 0.01 |
全部Total | 96.49 ± 4.27 | 9.13 ± 0.26 | 4.18 ± 0.10 | 1.62 ± 0.02 | 0.72 ± 0.02 | 0.74 ± 0.01 |
表2 基于3次随机抽样的芍药品种遗传多样性
Table 2 Genetic diversity of herbaceous peony cultivars based on three random sampling
抽样 Sampling | 样本数 Numbers of sampling (N) | 等位基因数 Number of observed alleles (Na) | 有效等位基因数 Number of effective alleles (Ne) | 香农信息指数 Shannon information index (I) | 观测杂合度 Observation heterozygosity (Ho) | 期望杂合度 Expected heterozygosity(He) |
---|---|---|---|---|---|---|
第1次1st | 29.75 ± 0.04 | 6.91 ± 0.15 | 3.98 ± 0.10 | 1.53 ± 0.02 | 0.71 ± 0.02 | 0.73 ± 0.01 |
第2次2nd | 29.75 ± 0.04 | 7.30 ± 0.15 | 4.04 ± 0.09 | 1.57 ± 0.02 | 0.72 ± 0.02 | 0.73 ± 0.01 |
第3次3rd | 29.75 ± 0.04 | 7.32 ± 0.15 | 4.12 ± 0.10 | 1.58 ± 0.02 | 0.71 ± 0.02 | 0.74 ± 0.01 |
全部Total | 96.49 ± 4.27 | 9.13 ± 0.26 | 4.18 ± 0.10 | 1.62 ± 0.02 | 0.72 ± 0.02 | 0.74 ± 0.01 |
栽培地 Culture place | 样本数 Numbers of sampling (N) | 等位基因数 Number of observed alleles (Na) | 有效等位基因数 Number of effective alleles (Ne) | 观测杂合度 Observation heterozygosity (Ho) | 期望杂合度 Expected heterozygosity (He) | 香农信息指数 Shannon information index(I) | 遗传分化系数 Genetic differentiation index(FST) | 基因流 Gene flow (Nm) |
---|---|---|---|---|---|---|---|---|
菏泽Heze | 165 | 10.79 | 4.57 | 0.75 | 0.77 | 1.74 | ||
洛阳Luoyang | 93 | 9.67 | 4.35 | 0.74 | 0.76 | 1.68 | ||
扬州Yangzhou | 97 | 9.48 | 4.14 | 0.68 | 0.74 | 1.62 | ||
临洮Lintao | 29 | 6.61 | 3.66 | 0.69 | 0.69 | 1.45 | ||
全部Total | 0.031 | 7.795 |
表3 基于SSR标记的不同栽培地芍药品种遗传多样性分析
Table 3 Genetic diversity analysis of herbaceous peony varieties from different cultivation areas based on SSR markers
栽培地 Culture place | 样本数 Numbers of sampling (N) | 等位基因数 Number of observed alleles (Na) | 有效等位基因数 Number of effective alleles (Ne) | 观测杂合度 Observation heterozygosity (Ho) | 期望杂合度 Expected heterozygosity (He) | 香农信息指数 Shannon information index(I) | 遗传分化系数 Genetic differentiation index(FST) | 基因流 Gene flow (Nm) |
---|---|---|---|---|---|---|---|---|
菏泽Heze | 165 | 10.79 | 4.57 | 0.75 | 0.77 | 1.74 | ||
洛阳Luoyang | 93 | 9.67 | 4.35 | 0.74 | 0.76 | 1.68 | ||
扬州Yangzhou | 97 | 9.48 | 4.14 | 0.68 | 0.74 | 1.62 | ||
临洮Lintao | 29 | 6.61 | 3.66 | 0.69 | 0.69 | 1.45 | ||
全部Total | 0.031 | 7.795 |
变异来源 Source of variation | 自由度 df | 方差总和 SS | 平均方差 MS | 变异组分 Est. Var. | 变异百分率/% Variation |
---|---|---|---|---|---|
栽培地间Among cultivation areas | 3 | 504.584 | 168.195 | 1.609 | 6 |
栽培地内Within cultivation areas | 382 | 10 017.584 | 26.224 | 26.224 | 94 |
总和Total | 385 | 10 522.168 | 27.833 | 100 |
表4 芍药386个品种在不同栽培地间的遗传变异分析
Table 4 Analysis of genetic variation of 386 herbaceous peonies varieties from different cultivation areas
变异来源 Source of variation | 自由度 df | 方差总和 SS | 平均方差 MS | 变异组分 Est. Var. | 变异百分率/% Variation |
---|---|---|---|---|---|
栽培地间Among cultivation areas | 3 | 504.584 | 168.195 | 1.609 | 6 |
栽培地内Within cultivation areas | 382 | 10 017.584 | 26.224 | 26.224 | 94 |
总和Total | 385 | 10 522.168 | 27.833 | 100 |
栽培地 Culture place | 菏泽 Heze | 洛阳 Luoyang | 扬州 Yangzhou | 临洮 Lintao |
---|---|---|---|---|
菏泽Heze | 0.931 | 0.969 | 0.957 | |
洛阳Luoyang | 0.072 | 0.950 | 0.884 | |
扬州Yangzhou | 0.032 | 0.051 | 0.933 | |
临洮Lintao | 0.044 | 0.123 | 0.069 |
表5 芍药各栽培地品种的遗传距离及遗传一致性
Table 5 Nei’s genetic distance and identity of herbaceous peony varieties from different cultivation areas
栽培地 Culture place | 菏泽 Heze | 洛阳 Luoyang | 扬州 Yangzhou | 临洮 Lintao |
---|---|---|---|---|
菏泽Heze | 0.931 | 0.969 | 0.957 | |
洛阳Luoyang | 0.072 | 0.950 | 0.884 | |
扬州Yangzhou | 0.032 | 0.051 | 0.933 | |
临洮Lintao | 0.044 | 0.123 | 0.069 |
图2 利用33对SSR标记得到的芍药品种聚类图 图例括号中的数字表示该聚类组群中的品种数量。
Fig. 2 Cluster of herbaceous peony varieties based on 33 pairs of SSR markers Numbers in parentheses in the legend indicate the number of cultivars in that cluster group.
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