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园艺学报 ›› 2022, Vol. 49 ›› Issue (7): 1582-1588.doi: 10.16420/j.issn.0513-353x.2021-0597

• 研究报告 • 上一篇    下一篇

紫橙色大白菜新种质的创制

张鲁刚(), 卢倩倩, 何琼, 薛一花, 马晓敏, 马帅, 聂姗姗, 杨文静   

  1. 旱区作物逆境生物学国家重点实验室,西北农林科技大学园艺学院,陕西杨凌 712100
  • 收稿日期:2022-02-28 修回日期:2022-03-24 出版日期:2022-07-25 发布日期:2022-07-29
  • 通讯作者: 张鲁刚 E-mail:lugangzh@163.com
  • 基金资助:
    国家重点研发计划项目(2016YFD0101701);国家重点研发计划项目(2017YFD0101802);杨凌种业创新中心重点研发项目(Ylzy-sc-04)

Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage

ZHANG Lugang(), LU Qianqian, HE Qiong, XUE Yihua, MA Xiaomin, MA Shuai, NIE Shanshan, YANG Wenjing   

  1. State Key Laboratory of Crop Stress Biology in Arid Regions,College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Received:2022-02-28 Revised:2022-03-24 Online:2022-07-25 Published:2022-07-29
  • Contact: ZHANG Lugang E-mail:lugangzh@163.com

摘要:

为了丰富大白菜种质资源,提高大白菜功能成分,创制一种同时富含花青素和类胡萝卜素的紫橙色大白菜新种质。以橙色大白菜自交系14S125为母本,紫心大白菜自交系14S838为父本进行杂交,F1单株自交,在F2群体中先用分子标记确定携带紫心基因和橙色基因的单株,进一步选择园艺性状和结球性优良的单株,通过连续3代单株自交选择,育成了外叶绿色、球叶紫橙色的大白菜新种质4份,分别是19ZS40-1、19ZS17-1、19ZS94和19SF19-4,将紫心基因和橙色基因聚合在了一起,并且在大白菜球叶中表达。本研究中创制了同时产生类胡萝卜素和花青素的紫橙色的大白菜新种质,提高了其营养价值,为大白菜的营养品质育种奠定了新的基础。

关键词: 大白菜, 球色, 类胡萝卜素, 花青素, 杂交, 分子标记

Abstract:

In order to enrich the germplasm resources of Chinese cabbage and improve the functional composition,and create a new purple-orange heading Chinese cabbage germplasm riched in anthocyanins and carotenoids simultaneously. A purple heading Chinese cabbage pure line 14S838 and an orange heading Chinese cabbage pure line 14S125 were hybridized,and their F2 was produced by single F1 plant selfing. Then the excellent heading plants carrying purple heading gene and orange heading gene in the F2 population were selected by molecular markers combining field horticultural traits observation,lately,the selected plants was self-crossed and selected for three generations continuously. Finally,four new Chinese cabbage germplasm with the green outer leaves and purple-orange heading leaves,such as 19ZS40-1,19ZS17-1,19ZS94 and 19SF19-4,were bred. This study first successfully aggregated the purple heading gene and orange heading gene in one plant of Chinese cabbage together,promote these two gene normal expression,and created the novel germplasms of Chinese cabbage simultaneously accumulated both carotenoids and anthocyanins,which has greatly improved the nutritional value,enrich the color heading Chinese cabbage breeding materials,laid a new foundation for the nutritional quality breeding of Chinese cabbage.

Key words: Chinese cabbage, leaf-head color, carotenoids, anthocyanins, hybridization, molecular marker

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