非呼吸跃变和呼吸跃变型甜瓜成熟果实的激素与转录组分析

doi:10.16420/j.issn.0513-353x.2024-0371

摘要/Abstract

摘要： 甜瓜果实按照成熟习性可分为非呼吸跃变和呼吸跃变两种类型，其不同的激素、糖类代谢等成熟相关调控机制不十分清楚。对非呼吸跃变和呼吸跃变型甜瓜成熟果实进行激素代谢物分析，发现非呼吸跃变型甜瓜果实中脱落酸（ABA）、1–氨基环烷羧酸（ACC）、反式玉米素核苷–5′–单磷酸二钠盐（tZRMP）和生长素含量较高；进一步对非呼吸跃变型和呼吸跃变型甜瓜成熟果实的差异代谢物进行KEGG富集分析，结果表明“代谢途径”“植物激素信号转导”和“各种生物碱的生物合成”在非呼吸跃变型和呼吸跃变型甜瓜成熟果实中差异较大。通过RNA-seq建库和qRT-PCR鉴定到CmARF，CmARR2-like，CmGAI-like，CmPP2C，CmACO和CmEIL与激素代谢差异表达相关。在非呼吸跃变型甜瓜果实成熟中，ABA、吲哚、ACC和GA起关键作用；但在呼吸跃变型甜瓜果实中，除ABA外，IAA、GA和ETH信号通路也参与了调控。

关键词: 甜瓜, 果实成熟, 植物激素, 转录组, 基因表达

Abstract: Melon can be divided into non-climacteric and climacteric according to mature habituation，but the most important hormones，sugar metabolites and genetic regulatory mechanisms remain elusive between non-climacteric and climacteric melon. Plant hormone metabolism detection revealed that abscisic acid（ABA），and the content of 1-aminocyclop ropanecarboxylic acid（ACC），trans-Zeatin Riboside-5′-monophosphate Disodium Salt（tZRMP）and auxin were higher in mature non-climacteric melon. The KEGG pathway enrichment analysis of these differentially expressed genes（DEGs）revealed“metabolic pathways”，“plant hormone signal transduction”and“biosynthesis of various alkaloids” were significantly different in the ripe fruits of non-climacteric and climacteric melons. The RNA-seq and qRT-PCR analyses were performed to identify important genes，enzymes and pathways that are involved in sugar and hormone metabolites. CmARF，CmARR2-like，CmGAI-like，CmPP2C，CmACO and CmEIL associated with plant hormone metabolism were identified in mature melon. ABA，Indole，ACC and GA pathways play key roles in non-climacteric melon. While besides ABA，IAA，GA，and ethylene（ETH）signaling pathways were also involved in regulating the maturation of climacteric melon.

Key words: melon, fruit ripening, phytohormone, transcriptome, gene expression

中图分类号:

S 652

唐伶俐, 贺玉花, 王庆涛, 安璐璐, 徐永阳, 张健, 孔维虎, 户克云, 赵光伟. 非呼吸跃变和呼吸跃变型甜瓜成熟果实的激素与转录组分析[J]. 园艺学报, 2025, 52(4): 883-896.

TANG Lingli, HE Yuhua, WANG Qingtao, AN Lulu, XU Yongyang, ZHANG Jian, KONG Weihu, HU Keyun, ZHAO Guangwei. Hormonal and Transcriptome Analysis of Ripe Fruits of Non-Climacteric and Climacteric Melon[J]. Acta Horticulturae Sinica, 2025, 52(4): 883-896.

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